LOCUS       pCFJ1360                3378 bp    DNA        circular     14-DEC-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     518 bases. 94 bases in phase. PATC density = 37
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(1876..1893)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     858..878
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      48..730
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2669..3328
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1043..1053
                     /locus_tag="Golden Gate 3A"
                     /label="Golden Gate 3A"
                     /ApEinfo_label="Golden Gate 3A"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1563..1573
                     /locus_tag="Golden Gate 3B"
                     /label="Golden Gate 3B"
                     /ApEinfo_label="Golden Gate 3B"
                     /ApEinfo_fwdcolor="#57bffe"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          1050..1566
                     /locus_tag="smu-1 intron (518 bases. 94 bases in phase.
                     PATC density = 37)"
                     /label="smu-1 intron (518 bases. 94 bases in phase. PATC
                     density = 37)"
                     /ApEinfo_label="smu-1 intron (518 bases. 94 bases in
                     phase. PATC density = 37)"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
       61 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      241 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
      301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      721 caacgcggcc tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc
      781 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccag
      841 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tatcaacttt
      901 gtatagaaaa gttgGTGAAT TGTAATACGA CTCACTATAG GGCGAATTaa ttCGAGCTCG
      961 GTACCcagct tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcagca
     1021 agtttgtaca aaaaagcagg ctggtctcac tttttttgaa aagtgcagtt tgtagtctaa
     1081 ttttcattta ttttcttttt aaaaaacgca tcaattttta aatattttgg gacaaaatcc
     1141 gaaaactgta ctaatttgta gtttgtaaaa ttaaaaaaaa aacgcaaaaa aatgttttca
     1201 aaaatgctta gaataaaaat aaaatttcta aaaaattgaa caaaataaac atttaaaaat
     1261 tcaaaaagtt tgaaaaaatg caaagttttt taatgcaaaa aatatttaat gtttaaacaa
     1321 atttaaaaaa tgttgtaaga atatgtttag agacagtttt taaatgttaa attgcaaaaa
     1381 aaaaaagcaa aaaaaaattt gaaaaatgta acagaaaatt ttaattaaaa aagtcgtgtt
     1441 tttttcaaac ctgcaattct ttgtaatttt atttataaag agaaacaaat caaattgtat
     1501 attacgaaaa acgatactaa aattcgaaaa tttgcggttt tttgcgtaaa aaatacggtt
     1561 ccgtaaagag accacccagc tttcttgtac aaagtggacc agcctcaaga acacccgaat
     1621 ggagtctcta agctacataa taccaactta cactttacaa aatgttgtcc cccaaaatgt
     1681 agccattcgt atctgctcct aataaaaaga aagtttcttc acattctaaa aaaaaaaaaa
     1741 aaaaaaaaaa aaaaaaaacc cccccccccc ctgcaggcat gcattaatta aaggccatga
     1801 tggccagcta gcttgagtat tcTATAGTGT CACCTAAATA GCTTGGCcaa ctttattata
     1861 catagttgat aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     1921 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1981 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     2041 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     2101 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     2161 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2221 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     2281 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2341 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3181 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3361 ttagattgat ttaaaact    
//
