LOCUS pMDJ22 10042 bp DNA circular 14-DEC-2020 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Synthetic Genome Biology lab @ KAUST www.wormbuilder.org COMMENT Aljohani et al. 2020 COMMENT Note: Removing backbone by PCR amplification or by digest is likely to improve germline expression and editing efficiency. Numbers in manuscript were generated with no amplification or linearization. COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 8208..8236 /locus_tag="AmpR promoter" /label="AmpR promoter" /ApEinfo_label="AmpR promoter" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 2713..2976 /locus_tag="Cre(smu-1, no NLS)" /label="Cre(smu-1, no NLS)" /ApEinfo_label="Cre(smu-1, no NLS)" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 8278..9138 /locus_tag="ampR" /label="ampR" /ApEinfo_label="ampR" /ApEinfo_fwdcolor="#ff6633" /ApEinfo_revcolor="#ff6633" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 4093..4392 /locus_tag="Cre(smu-1, no NLS)(1)" /label="Cre(smu-1, no NLS)(1)" /ApEinfo_label="Cre(smu-1, no NLS)" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(7683..7700) /locus_tag="M13-fwd" /label="M13-fwd" /ApEinfo_label="M13-fwd" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" 3'UTR 4422..4666 /locus_tag="gpd-2 operon intergenic" /label="gpd-2 operon intergenic" /ApEinfo_label="gpd-2 operon intergenic" /ApEinfo_fwdcolor="#f76ca1" /ApEinfo_revcolor="#f76ca1" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 1..21 /locus_tag="M13-rev" /label="M13-rev" /ApEinfo_label="M13-rev" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" sig_peptide 4639..4646 /locus_tag="trans splice" /label="trans splice" /ApEinfo_label="trans splice" /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 9233..9915 /locus_tag="ColE1 origin" /label="ColE1 origin" /ApEinfo_label="ColE1 origin" /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" 3'UTR 6667..7654 /locus_tag="smu-1 3'UTR" /label="smu-1 3'UTR" /ApEinfo_label="smu-1 3'UTR" /ApEinfo_fwdcolor="#f76ca1" /ApEinfo_revcolor="#f76ca1" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 58..2121 /locus_tag="Psmu-2" /label="Psmu-2" /ApEinfo_label="Psmu-2" /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="#ff8000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 3299..3561 /locus_tag="Cre(smu-1, no NLS)(2)" /label="Cre(smu-1, no NLS)(2)" /ApEinfo_label="Cre(smu-1, no NLS)" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 2278..2407 /locus_tag="Cre(smu-1, no NLS)(3)" /label="Cre(smu-1, no NLS)(3)" /ApEinfo_label="Cre(smu-1, no NLS)" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 2150..2224 /locus_tag="Cre(smu-1, no NLS)(4)" /label="Cre(smu-1, no NLS)(4)" /ApEinfo_label="Cre(smu-1, no NLS)" /ApEinfo_fwdcolor="#0080ff" /ApEinfo_revcolor="#0080ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS join(4667..4735,4780..5002,5308..5444,5767..5961, 6493..6666) /locus_tag="tagRFP-T (myristylated)" /label="tagRFP-T (myristylated)" /ApEinfo_label="tagRFP-T (myristylated)" /ApEinfo_fwdcolor="#cc3333" /ApEinfo_revcolor="#cc3333" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgtgc 61 accaatttga gtttccatgg ggaatcaagc ggataccgat tgagttgaag aaccctaaat 121 aaaattttat atattttacc ttccaacttc gaaaaaatca ctcaccattt tagcatgttt 181 ccctcaaaaa ccgtggcaaa ttgctcttgg aaaataatag acacatcagt tggtcgagca 241 ttttttggag gaattttaaa actgtgatga tggttgaagg tgactatatt gtctgcgagt 301 tgatactctg gaatattgat ttttttgaag atttcatagt cttcttggat ttcaaaaatt 361 gaaatatttc agctgtatta aaagagaata aataaaatga tattgcctag gggtgagcgg 421 caaacgattt tttccggcaa atcggcaaat cgacaaattg ccggaattgg aaatttccag 481 caaatcggca tatcggcaat ttgccggaat ttaaaatttc cggcaaaccg gcaaaccggc 541 aaattgccga tttgccgaac ttcggtaaat ttgcaaatcg gtaatttgcc gaaaattttc 601 ggcaaattgt ggttttgcac ttattttttg gaatttcaat ttcaaatggc aaaattaaac 661 gcatcatatg aatttttcta tatctatttt gagaagttct atgaaaatat gtagaaaaaa 721 cgggaaaaat tcaataatag acagttttaa gtgcttccgt cttataaaaa atccctctaa 781 acatttccgg caaatctggt attcggcaaa tcggcaagtc ggcaatttgc cgtaaattaa 841 aatttccggc aaaacggcaa accggcaaat tgccggaatt ggaaatttcc ggcaaatcgg 901 caaattgctt gcccattttt tgattcttag tcggcgtttt acacaaaaga taaattccaa 961 taaaagacag aactttgctt tttgccagaa attaaaaaat tggggcaaac caatccaaat 1021 atgaagtgta aacttgggtt tgcaccaggg ctgtgcggaa accggcaatt gccgaaattg 1081 ccgaacccta aaaactttgg caaccggcaa cttttgctgg ctggaacttc ggcaactttt 1141 cggcaaacgg caacttggtt gccggttgcg caaagctctg gtttgcgttt agtagcaagg 1201 cttcctgaaa gaattccgga ggaaaactcc ccctaactca ccttttactc tatgcatctg 1261 cttaattttg tgcaattgta gtgcaaatag taggaaatga attttttttc cgccattttt 1321 tccttttcct atttctgctt ttcaatttcc acgtggagct cttttaaaat gaaaatttaa 1381 actctagata tttcacgttt taaaaaaaaa aaattttaat tttctatcgg ttttactttt 1441 gacgaaaagg gttcacagac attggaaata atcatcattt actatatata aagcgcttat 1501 tctgtgtgtc catagtttgt agtctctgta gtctttgtag tctgtgaagt tttggcttct 1561 ggagggatag tgagttgggg tcagtgtagg gatatagtcg gggtactgta gtggtacaat 1621 agtggtacgg taggaatact gtagggttac ggtagtttca taaaaaatta attttcagcc 1681 ccagaagtcg ggggccgcgc cgaaggtgcc tagacctaag cctaggcctc agcctaagcc 1741 taattacatc aaaaaagcta tgacttttgg atcattttga cttcttttct agaacgcata 1801 atgaaataat tcttaatttc atcggctttt tctcatcgtt gcttagaagt attgtcttga 1861 aaattgatat ttttagaaag tacacatatt tctcaatcct tttatttaaa tttctgaaag 1921 aaactttgcc acaacggtga gaaaagggtg cccgaaaaat gtgcatttaa atgtgaaaaa 1981 tatccgtggc cgcggcctaa gaacccccgt cggccatgtg gtgcaagtgc gctctgttgc 2041 ggatttttat atttaattgc attttcgcat attcgaacga ttttcctgat tttccagccg 2101 aaattgtcca ttaaaaagtg gCAAGTTTGT ACAAAAAAGC AGGCTAAAAA TGTCCAACCT 2161 CCTCACCGTC CACCAAAACC TCCCAGCCCT CCCAGTCGAC GCCACCTCCG ACGAGGTCCG 2221 TAAGGTATTT TCCTGCATTT TTCAACTGGG AAAATGAAAG AAAATCGATA ATTTCAGAAC 2281 CTCATGGACA TGTTCCGTGA CCGTCAAGCC TTCTCCGAGC ACACCTGGAA GATGCTCCTC 2341 TCCGTCTGCC GTTCCTGGGC CGCCTGGTGC AAGCTCAACA ACCGTAAGTG GTTCCCAGCC 2401 GAGCCAGGTA AGTTTTCTTA TGGGAAAGAA GGAAAAAACC GAGATTTTAC TTGAAAAATT 2461 GAATTTTTCG CGGGATTTTC ACCAAAAATT GTTGAATATT CATTATTTCA CGCTGTAAAA 2521 CAAAAAAAAA AAAAATCAAA AACTACGTTG AAATCGCGTT TTTAAGCGAA TTTTCTTCAG 2581 AATTGCCAGA TTTTAACCCC AAATTTTGCA GTTTTTAAAT AAAATTTCAC CTTTTCGGCT 2641 CAAATTGTAG ATTTTCCTGA AAATTTAGTA CAAAAAACAA TTTCCTCGTA AATTTTTCAA 2701 ATAGATTTTC AGAGGACGTC CGTGACTACC TCCTCTACCT CCAAGCCCGT GGACTCGCCG 2761 TCAAGACCAT CCAACAACAC CTCGGACAAC TCAACATGCT CCACCGTCGT TCCGGACTCC 2821 CACGTCCATC CGACTCCAAC GCCGTCTCCC TCGTCATGCG TCGTATCCGT AAGGAGAACG 2881 TCGACGCCGG AGAGCGTGCC AAGCAAGCCC TCGCCTTCGA GCGTACCGAC TTCGACCAAG 2941 TCCGTTCCCT CATGGAGAAC TCCGACCGTT GCCAAGGTAA GTTATGTCTA CCTGCCTGCC 3001 TACCGCCTAA ATTTTTTGTG AAGTTTCTTC AAAAAATCCA GAAAAAAAAA CAATTTTCAT 3061 ACGATTTTTT CCCTTAAAAT TGTGAATTTT CATGCTTTTT AGCCCCAAAA GTCATTATTT 3121 GAGAAAAAAT TTCATACAAA AAAGTTTTGA GAAATACACA ATTTTTTAAA TGTAATTTTC 3181 AAATTTTCAA TTTTCAACTA GAAAATTCAC AAAACTTGTA AATTTTGGAC CAAAACATTT 3241 ATACAATTAC TTTTTTTGAA TCTAATAACT ACAATAACTA CAAATTTAAC ATTTTCAGAC 3301 ATCCGTAACC TCGCCTTCCT CGGAATCGCC TACAACACCC TCCTCCGTAT CGCCGAGATC 3361 GCCCGTATCC GTGTCAAGGA CATCTCCCGT ACCGACGGAG GACGTATGCT CATCCACATC 3421 GGACGTACCA AGACCCTCGT CTCCACCGCC GGAGTCGAGA AGGCCCTCTC CCTCGGAGTC 3481 ACCAAGCTCG TCGAGCGTTG GATCTCCGTC TCCGGAGTCG CCGACGACCC AAACAACTAC 3541 CTCTTCTGCC GTGTCCGTAA GGTAAGTTCT TTTTTTGAAA AGTGCAGTTT GTAGTCTAAT 3601 TTTCATTTAT TTTCTTTTTA AAAAACGCAT CAATTTTTAA ATATTTTGGG ACAAAATCCG 3661 AAAACTGTAC TAATTTGTAG TTTGTAAAAT TAAAAAAAAA ACGCAAAAAA ATGTTTTCAA 3721 AAATGCTTAG AATAAAAATA AAATTTCTAA AAAATTGAAC AAAATAAACA TTTAAAAATT 3781 CAAAAAGTTT GAAAAAATGC AAAGTTTTTT AATGCAAAAA ATATTTAATG TTTAAACAAA 3841 TTTAAAAAAT GTTGTAAGAA TATGTTTAGA GACAGTTTTT AAATGTTAAA TTGCAAAAAA 3901 AAAAAGCAAA AAAAAATTTG AAAAATGTAA CAGAAAATTT TAATTAAAAA AGTCGTGTTT 3961 TTTTCAAACC TGCAATTCTT TGTAATTTTA TTTATAAAGA GAAACAAATC AAATTGTATA 4021 TTACGAAAAA CGATACTAAA ATTCGAAAAT TTGCGGTTTT TTGCGTAAAA AATACGGTTC 4081 CGTAATTTTC AGAACGGAGT CGCCGCCCCA TCCGCCACCT CCCAACTCTC CACCCGTGCC 4141 CTCGAGGGAA TCTTCGAGGC CACCCACCGT CTCATCTACG GAGCCAAGGA CGACTCCGGA 4201 CAACGTTACC TCGCCTGGTC CGGACACTCC GCCCGTGTCG GAGCCGCCCG TGACATGGCC 4261 CGTGCCGGAG TCTCCATCCC AGAGATCATG CAAGCCGGAG GATGGACCAA CGTCAACATC 4321 GTCATGAACT ACATCCGTAA CCTCGACTCC GAGACGGGAG CCATGGTCCG TCTCCTCGAG 4381 GACGGAGACT AGACCCAGCT TTCTTGTACA AAGTGGCCTA Agctgtctca tcctactttc 4441 acctagttaa ctgcttgtct taaaatctat gcttctcttt agtatctaaa attttcctag 4501 aagcttacaa gtatataaat ggtctcttct caataaaggt tgtatattta ttcatcttat 4561 tgaatctgcc atttcctcgt ttttgcgagt ttatatacct tccaattttc tttctattgt 4621 attttcaact tctaatttta attcagggaa actgcttcaa cgcatcATGG GTTGTACCAT 4681 GTCACAGGAA GAGCGTGCCG CTCTTGAAAG ATCACGAATG TCCTCAGTCT CCAAGgtaac 4741 acttagtttt tgttgctctg tttaaaaatt aattttaagG GAGAGGAGCT CATCAAGGAG 4801 AACATGCACA TGAAGCTCTA CATGGAGGGA ACCGTCAACA ACCACCACTT CAAGTGCACC 4861 TCCGAGGGAG AGGGAAAGCC ATACGAGGGA ACCCAAACCA TGCGTATCAA GGTCGTCGAG 4921 GGAGGACCAC TCCCATTCGC CTTCGACATC CTCGCCACCT CCTTCATGTA CGGATCGCGT 4981 ACCTTCATCA ACCACACCCA AGgtaagttt tcttatggga aagaaggaaa aaaccgagat 5041 tttacttgaa aaattgaatt tttcgcggga ttttcaccaa aaattgttga atattcatta 5101 tttcacgctg taaaacaaaa aaaaaaaaaa tcaaaaacta cgttgaaatc gcgtttttaa 5161 gcgaattttc ttcagaattg ccagatttta accccaaatt ttgcagtttt taaataaaat 5221 ttcacctttt cggctcaaat tgtagatttt cctgaaaatt tagtacaaaa aacaatttcc 5281 tcgtaaattt ttcaaataga ttttcagGAA TCCCAGACTT CTTCAAGCAA TCCTTCCCAG 5341 AGGGATTCAC CTGGGAGCGT GTCACCACCT ACGAGGACGG AGGAGTCCTC ACCGCCACCC 5401 AAGACACCTC CCTCCAAGAC GGATGCCTCA TCTACAACGT CAAGgtaagt tatgtctacc 5461 tgcctgccta ccgcctaaat tttttgtgaa gtttcttcaa aaaatccaga aaaaaaaaca 5521 attttcatac gattttttcc cttaaaattg tgaattttca tgctttttag ccccaaaagt 5581 cattatttga gaaaaaattt catacaaaaa agttttgaga aatacacaat tttttaaatg 5641 taattttcaa attttcaatt ttcaactaga aaattcacaa aacttgtaaa ttttggacca 5701 aaacatttat acaattactt tttttgaatc taataactac aataactaca aatttaacat 5761 tttcagATCC GTGGAGTCAA CTTCCCATCC AACGGACCAG TCATGCAAAA GAAGACCCTC 5821 GGATGGGAGG CCAACACCGA GATGCTCTAC CCAGCCGACG GAGGACTGGA GGGACGTACC 5881 GACATGGCCC TCAAGCTCGT CGGAGGAGGA CACCTCATCT GCAACTTCAA GACCACCTAC 5941 CGTTCCAAGA AGCCAGCCAA Ggtaagttct ttttttgaaa agtgcagttt gtagtctaat 6001 tttcatttat tttcttttta aaaaacgcat caatttttaa atattttggg acaaaatccg 6061 aaaactgtac taatttgtag tttgtaaaat taaaaaaaaa acgcaaaaaa atgttttcaa 6121 aaatgcttag aataaaaata aaatttctaa aaaattgaac aaaataaaca tttaaaaatt 6181 caaaaagttt gaaaaaatgc aaagtttttt aatgcaaaaa atatttaatg tttaaacaaa 6241 tttaaaaaat gttgtaagaa tatgtttaga gacagttttt aaatgttaaa ttgcaaaaaa 6301 aaaaagcaaa aaaaaatttg aaaaatgtaa cagaaaattt taattaaaaa agtcgtgttt 6361 ttttcaaacc tgcaattctt tgtaatttta tttataaaga gaaacaaatc aaattgtata 6421 ttacgaaaaa cgatactaaa attcgaaaat ttgcggtttt ttgcgtaaaa aatacggttc 6481 cgtaattttc agAACCTCAA GATGCCAGGA GTCTACTACG TCGACCACCG TCTGGAGCGT 6541 ATCAAGGAGG CCGACAAGGA GACGTACGTC GAGCAACACG AGGTCGCCGT CGCCCGTTAC 6601 TGCGACCTCC CATCCAAGCT CGGACACAAG CTCAACGGAA TGGACGAGCT CTACAAGTCG 6661 GGATAAtttt ccccagattt ccccacgatt ttttttgaca tttttatgaa aattttctct 6721 aaaagttacc cctattttgt ttagactgat ctcttatttt atccaatgtt tcaaattttt 6781 gaaattgctc gaaaattcca attttcacat atttttgttg attttttcgc cattttcccc 6841 tgataaatta acttattttt gcaatttttt ctttcgaaaa aatattggtt tctcatcacc 6901 atttatcctt ttttttatgg agttaaaaat tgttaaaaaa atttaatttt ttttctaatt 6961 ttagagtcaa aacgggttcg aatctagagc gtagctgtac gattttttgg cgatttttgc 7021 ggttttctgg tgagtttttt taaagttatt tttttgaaaa aaattcttga aaataaaagg 7081 aaattgtttt aaaaaaaaat ttcaaagaaa aaattttgaa aaaattgaaa catttttttt 7141 attttgacca aaaatattga aaattaatta acattttttt ttggaaagtt aatttttaat 7201 acaaattttt taaatttatt ttacaaaaaa aattgaattt ctgtgaaaaa tcagttttcc 7261 agtacataat gatttgacca aaaaaaattg aaaaaaatga ttttttaaaa ttaaaaatac 7321 cataaaatgc tgaaaattca gaaaaataag taaattttaa aaaattctgg aaaaacatct 7381 aaaaaccccc caaatataat attttggacc cttttatttt ctgctcgaca tctcaaatat 7441 cctccaattt ttcgcttttt tgattaattg aaagcatagt gggggcaggt gttataggac 7501 acccttccct ccactttttt ggagcatttt tcaaagctct tccgagagca gaagcttctc 7561 aatttttcca ccccaaaagt ttgaaattct gccaaaaaat ctcgcatttt aacaattttt 7621 cgagatgttt cacgtccttt tcattgcgat ttcccaactt tattatacat agttgataat 7681 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 7741 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 7801 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc 7861 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct 7921 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg 7981 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 8041 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc 8101 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 8161 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 8221 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 8281 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 8341 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 8401 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 8461 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 8521 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 8581 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 8641 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 8701 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 8761 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 8821 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 8881 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 8941 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggctctcgc 9001 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 9061 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 9121 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 9181 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 9241 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 9301 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 9361 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 9421 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 9481 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 9541 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 9601 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 9661 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 9721 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 9781 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 9841 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 9901 gccagcaacg cggccttttt acggttcctg gtcttttgct ggccttttGC TCACATGTTC 9961 TTTCCTGCgt tatcccctga ttctgtggat aaccgtatta ccgccttTGA GTGAGCTGAT 10021 ACCAGCGgat aacaatttca ca //