LOCUS       pMNK56___T774___        3565 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Unpublished reagents Monika Priyadarshini and Christian
            Froekjaer-Jensen.
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     piRNA-mediated RNA interference (piRNAi)
COMMENT     Co-injection of 1.4 kb PCR fragment generated with UniF/UniR will
            inactivate him-8 and produce approx. 40% males in transgenic
            animals.
COMMENT     Note: Plasmid injection will NOT give penetrant phenotype.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(1196..1215)
                     /locus_tag="him-8"
                     /label="him-8"
                     /ApEinfo_label="him-8"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#fc6fce"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1196..1215
                     /locus_tag="him-8_6"
                     /label="him-8_6"
                     /ApEinfo_label="him-8_6"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             522..541
                     /locus_tag="him-8(1)"
                     /label="him-8(1)"
                     /ApEinfo_label="him-8"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#fc6fce"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(173..192)
                     /locus_tag="him-8(2)"
                     /label="him-8(2)"
                     /ApEinfo_label="him-8"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#fc6fce"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     173..192
                     /locus_tag="him-8_4"
                     /label="him-8_4"
                     /ApEinfo_label="him-8_4"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(846..865)
                     /locus_tag="him-8(3)"
                     /label="him-8(3)"
                     /ApEinfo_label="him-8"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#fc6fce"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     846..865
                     /locus_tag="him-8_3"
                     /label="him-8_3"
                     /ApEinfo_label="him-8_3"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     1195..1195
                     /locus_tag="21ur-3471_11_female_116"
                     /label="21ur-3471_11_female_116"
                     /ApEinfo_label="21ur-3471_11_female_116"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1072..1091
                     /locus_tag="him-8(4)"
                     /label="him-8(4)"
                     /ApEinfo_label="him-8"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#fc6fce"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(542..542)
                     /locus_tag="21ur-1224_4_female_46"
                     /label="21ur-1224_4_female_46"
                     /ApEinfo_label="21ur-1224_4_female_46"
                     /ApEinfo_fwdcolor="#fc6665"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1072..1091)
                     /locus_tag="him-8_2"
                     /label="him-8_2"
                     /ApEinfo_label="him-8_2"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     845..845
                     /locus_tag="21ur-488_-9_male_386"
                     /label="21ur-488_-9_male_386"
                     /ApEinfo_label="21ur-488_-9_male_386"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="#66ccfe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             353..372
                     /locus_tag="him-8(5)"
                     /label="him-8(5)"
                     /ApEinfo_label="him-8"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#fc6fce"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1092..1092)
                     /locus_tag="21ur-2948_-12_male_233"
                     /label="21ur-2948_-12_male_233"
                     /ApEinfo_label="21ur-2948_-12_male_233"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="#66ccfe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(353..372)
                     /locus_tag="him-8_1"
                     /label="him-8_1"
                     /ApEinfo_label="him-8_1"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(373..373)
                     /locus_tag="21ur-1089_-12_male_62"
                     /label="21ur-1089_-12_male_62"
                     /ApEinfo_label="21ur-1089_-12_male_62"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="#66ccfe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(522..541)
                     /locus_tag="him-8_5"
                     /label="him-8_5"
                     /ApEinfo_label="him-8_5"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     172..172
                     /locus_tag="21ur-421_2_not enriched_1507"
                     /label="21ur-421_2_not enriched_1507"
                     /ApEinfo_label="21ur-421_2_not enriched_1507"
                     /ApEinfo_fwdcolor="#fc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1418..1435)
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3514..3534
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2754..3342
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1366..1387)
                     /locus_tag="UniF"
                     /label="UniF"
                     /ApEinfo_label="UniF"
                     /ApEinfo_fwdcolor="#75d5ff"
                     /ApEinfo_revcolor="#d783ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1548..2513)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#75d5ff"
                     /ApEinfo_revcolor="#75d5ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1..21
                     /locus_tag="uniR"
                     /label="uniR"
                     /ApEinfo_label="uniR"
                     /ApEinfo_fwdcolor="#75d5ff"
                     /ApEinfo_revcolor="#d783ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 cactgagcct ccacctagcc tcgcgcttga cgcgctagtc aactaacata aaaaaggtga
       61 aacattgcga ggatacatag aaaaaacaat acttcgaatt catttttcaa ttacaaatcc
      121 tgaaatgttt cactgtgttc ctataagaaa acattgaaac aaaatattAa gTACAAGTGT
      181 GCTTGCATTT TTctaatttt gattttgatt ttgaaatcga atttgcaaat ccaattaaaa
      241 atcattttct gataattaga cagttcctta tcgttaattt tattatatct atcgagttag
      301 aaattgcaac gaagataatg tcttccaaat actgaaaatt tgaaaatatg ttGAAACATT
      361 ATGGATTGAT TGAttGccag aactcaaaat atgaaatttt tatagttttg ttgaaacagt
      421 aagaaaatct tgtaattact gtaaactgtt tgcttttttt aaagtcaacc tacttcaaat
      481 ctacttcaaa aattataatg tttcaaatta cataactgtg tATTATGAAT GCTTGTTTCG
      541 CActgtagag cttcaatgtt gataagattt attaacacag tgaaacaggt aatagttgtt
      601 tgttgcaaaa tcggaaatct ctacatttca tatggttttt aattacaggt ttgttttata
      661 aaataattgt gtgatggata ttattttcag acctcatact aatctgcaaa ccttcaaaca
      721 atatgtgaag tctactctgt ttcactcaac cattcatttc aatttggaaa aaaatcaaag
      781 aaatgttgaa aaattttcct gtttcaacat tatgacaaaa atgttatgat tttaataaaa
      841 aCaaTAAATG TAGGACTTCG TAATGttctg tttttcttag aagtgttttc cggaaacgcg
      901 taattggttt tatcacaaat cgaaaacaaa caaaaatttt tttaattatt tctttgctag
      961 ttttgtagtt gaaaattcac tataatcatg aataagtgag ctgcccaagt aaacaaagaa
     1021 aatttggcag cggccgacaa ctaccgggtt gcccgattta tcagtggagg aTTTCTCCAC
     1081 TCCAATCGTT CAtcTaatgt gatgtacacg gttttcattt aaaaacaaat tgaaacagaa
     1141 atgactacat tttcaaattg tctatttttg ctgtgtttat tttgccacca acaaTCACTT
     1201 CTCTTGGACT TTGACtcaat ctagtaaact cacttaatgc aattcctcca gccacatatg
     1261 taaacgttgt atacatgcag aaaacggttt tttggtttta atgggaactt ttgacaaatt
     1321 gttcgaaaat cttaagctgt cccatttcag ttgggtgatc gatttaggtc aggcggaatg
     1381 gcacttcctt attccagatg cgtgcggatt atgcatgact ggccgtcgtt ttacaacgtc
     1441 gtgactggga aaaccctggc gcttgtggtg gatcacagtt attggagact tccttggccg
     1501 tacgatgggt tttgtttcag gtatctggca cttttcgggg aaatgtgCGC GGAACCCCTA
     1561 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
     1621 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC
     1681 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA
     1741 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA
     1801 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT
     1861 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG
     1921 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC
     1981 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA
     2041 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT
     2101 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG
     2161 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA
     2221 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG
     2281 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG
     2341 CTGATAAATC TGGAGCCGGT GAGCGTGGCT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG
     2401 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG
     2461 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAActgtcag
     2521 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2581 tctaggtgaa gatccttgaa ttgccattat caacaagttt gtacaaaaaa gcaggctccg
     2641 aattggtatc acgaggacga tttgaagccc cctacccatt tacgctgact tgacgggacg
     2701 gcggctttgt tgaatagagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2761 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2821 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2881 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2941 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3001 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3061 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3121 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3181 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3241 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3301 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3361 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc agcgacatat
     3421 gccatacgcc gaactgaact ccacctaatg tcttggagat tgtcggatag cgcggaatag
     3481 gattgtgccc tctgagcgga taacaatttc acacaggaaa cagctatgac catgacacat
     3541 gcatccacca tcgcagactt atcat   
//
