LOCUS       pCFJ2560___pEXP_        7931 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Unpublished reagents. Sonia el Mouridi and Christian
            Froekjaer-Jensen. Synthetic Genome Biology lab. KAUST.
COMMENT     www.wormbuilder.org
COMMENT     Positive control targeting construct for mosTI with split unc-119
            selection.
COMMENT     Broad somatic and germline fluorescence. Screen for unc-119 rescue.
COMMENT     GFP is codon-optimized and contains PATCs for germline expression. 
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(70..369,421..527)
                     /locus_tag="cbr-unc-119 (non-rescuing exons 3-4)"
                     /label="cbr-unc-119 (non-rescuing exons 3-4)"
                     /ApEinfo_label="cbr-unc-119 (non-rescuing exons 3-4)"
                     /ApEinfo_fwdcolor="#9437ff"
                     /ApEinfo_revcolor="#9437ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     48..69
                     /locus_tag="UniF"
                     /label="UniF"
                     /ApEinfo_label="UniF"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     1..17
                     /locus_tag="M13F"
                     /label="M13F"
                     /ApEinfo_label="M13F"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(5836..5852)
                     /locus_tag="M13R"
                     /label="M13R"
                     /ApEinfo_label="M13R"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(5780..5800)
                     /locus_tag="UniR"
                     /label="UniR"
                     /ApEinfo_label="UniR"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6853..7818
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#929292"
                     /ApEinfo_revcolor="#929292"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6024..6612
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           528..640
                     /locus_tag="eft-3 3'UTR"
                     /label="eft-3 3'UTR"
                     /ApEinfo_label="eft-3 3'UTR"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#d783ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     3'UTR           5097..5428
                     /locus_tag="tbb-2 3'UTR"
                     /label="tbb-2 3'UTR"
                     /ApEinfo_label="tbb-2 3'UTR"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#d783ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5480..5779
                     /locus_tag="Spacer (no stop, no PolyA)"
                     /label="Spacer (no stop, no PolyA)"
                     /ApEinfo_label="Spacer (no stop, no PolyA)"
                     /ApEinfo_fwdcolor="#75d5ff"
                     /ApEinfo_revcolor="#75d5ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(1356..1418,1463..1679,2589..2683,3657..3833,
                     4778..5101)
                     /locus_tag="ceGFP(PATC, 2xNLS)"
                     /label="ceGFP(PATC, 2xNLS)"
                     /ApEinfo_label="ceGFP(PATC, 2xNLS)"
                     /ApEinfo_fwdcolor="#d4fb78"
                     /ApEinfo_revcolor="#d4fb78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     transit_peptide 1392..1412
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#ff84ff"
                     /ApEinfo_revcolor="#ff84ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     transit_peptide 4994..5065
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff84ff"
                     /ApEinfo_revcolor="#ff84ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        734..1359
                     /locus_tag="Peft-3 (w ATG)"
                     /label="Peft-3 (w ATG)"
                     /ApEinfo_label="Peft-3 (w ATG)"
                     /ApEinfo_fwdcolor="#72fcd5"
                     /ApEinfo_revcolor="#72fcd5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc
       61 gcctgacctA AGTGCTTTTC GAGATCGCCA AACCGGAGAA CGATCAGGAG AATGATGAGT
      121 CGCCACAGGA GTCGGCAAGA TACGTGCGTT ATAGATTTGC TCCAAACTTT TTGAAACTCA
      181 AAACGGTCGG AGCAACTGTG GAATTCAAAG TAGGAGACAT CCCAATCCAT CATTTCCGAA
      241 TGATCGAACG TCACTTCTTC AAAGATCGCC TTCTGAAGTG TTTTGACTTT GAATTCGGAT
      301 TCTGTATTCC GAATTCACGA AACAACTGTG AACATATCTA TGAGTTCCCT CAACTCTCTC
      361 AACAACTCAg tgagtcatta ttctaaaaag tacaattcaa aagactaatc tctttttcag
      421 TGGACGACAT GATCAACAAT CCAAACGAGA CTCGTTCTGA CAGCTTCTAT TTCGTCGATA
      481 ACAAACTCGT CATGCACAAC AAAGCCGACT ACTCATATGA TGCATAAatc ttcattgttg
      541 agtttatctt gttgattttt gaataaatta tcaactcttt actttttaat gggttatgaa
      601 ataaataaac attgaaaact gataaacaac gttcatctct ataacttcgt atagcataca
      661 ttatacgaag ttatTGCCAT AGCATGGCCT TTGCAGGatt acgccaagct atcaactttg
      721 tatagaaaag ttgGCACCTT TGGTCTTTTA TTGTCAACTT CCATTGGTTC TTCCATtgtt
      781 tctgttaaat taatgaattt ttcataaaat aaagacatta tacaatataa aaatgaagaa
      841 tttattgaaa ataaactgcc agagagaaaa agtatgcaac actcccgccg agagtgtttg
      901 aaatggtgta cggtacattt tcgtgctagg agttagatgt gcaggcagca acgagagggg
      961 gagagatttt tttgggcctt gtgaaattaa cgtgagtttt ctggtcatct gactaatcat
     1021 gttggttttt tgttggttta ttttgttttt atctttgttt ttatccagat taggaaattt
     1081 aaattttatg aatttataat gaggtcaaac attcagtccc agcgtttttc ctgttctcac
     1141 tgtttagtcg aatttttatt ttaggctttc aacaaatgtt ctaactgtct tatttgtgac
     1201 ctcacttttt atattttttt aatttttaaa aatattagaa gtttctagga taattttttc
     1261 gacttttatt ctctctaccg tccgcactct tcttactttt aaattaaatt gttttttttt
     1321 cagttgggaa acactttgct cactccgtag cagccATGGC AAGTTTGTAC AAAAAAGCAG
     1381 GCTCGGGATC CCCAAAGAAG AAGCGTAAGG TGTCCAAGgt aacacttagt ttttgttgct
     1441 ctgtttaaaa attaatttta agGGAGAGGA GCTGTTCACC GGAGTCGTCC CAATCCTCGT
     1501 CGAGCTCGAC GGGGACGTCA ACGGACACAA GTTCTCCGTG TCCGGAGAGG GAGAGGGAGA
     1561 TGCCACCTAC GGAAAGCTCA CCCTCAAGTT CATCTGCACC ACCGGAAAGC TCCCAGTCCC
     1621 ATGGCCAACC CTCGTCACCA CCTTCTGCTA CGGAGTCCAA TGCTTCTCCC GTTACCCAGg
     1681 taagttttct gttttattta cttgttttga gaattgggat ttttagctga aaaattggat
     1741 tttaagatga aaaattgaaa ttttcaacta aaaatttcaa tttttagctg aaaagttaaa
     1801 tttttagcta aagagtggga tttttagttg aaaaatcgag atttctagct aaaaagtatg
     1861 atttttagct gaaagattgg aattttaagc agaaaaaatt gtatttttag ctgataaatc
     1921 aaattttaag cggaaaaatt tgaattttta gctaaaaaat tccattttca actaaaaatc
     1981 gtgattttta gctttaaaaa ttggattttt agctgaaaaa tcggattttt agctgaaaat
     2041 tgaaattttt tagttaaaaa gtgagatttt ttaacagaaa agtgagattt ttagttaaaa
     2101 aataatattt ttagctaaaa aatacaattt tcaactaaag atcgtgattt ttagctttaa
     2161 aaattggatt tttagctaaa aaatcggatt tttagctgaa aattgaaatt ttttagttaa
     2221 aaagtgggat tttaagatga aaagtgagat tttttagctg aaaagtgaga ttttcagcta
     2281 aaaaatacaa ttttcaactg aaaaattggg atttttagct ttaaaaattg gattttcagc
     2341 tttaaaaatt ggatttttag ctgaaaagtg agatttttag ttgaaaaatt gaaatgttta
     2401 gctgaaaaat tggaattttt aagtaaaaaa gtgggatttt aagatgaaaa gtgagatgtt
     2461 tttagctgaa aagtgagatt tttagctaaa aaatacaatt ttcaactgaa aaattgggat
     2521 ttttagctca aaagtgggac gaaagacgaa aaaaaaaatc gattttttac caaaaattag
     2581 attttcagAC CACATGAAGC GTCACGACTT CTTCAAGTCC GCCATGCCAG AGGGATACGT
     2641 CCAAGAGCGT ACCATCTTCT TCAAGGACGA CGGAAACTAC AAGgtaagtt atgtacgcga
     2701 tttctcggcg aaaaaatgga cttttttgct caaaaactga atttttcagc tttttttgca
     2761 ctgaaaatgc tgaaaattgg tttttttgct gaaaattcac cgaaaaaagc tgaaaatatt
     2821 gcagaaatcg acgaaaaatg ccttttttgc tgaaatttca gccagaaaat tgttcagaat
     2881 cgcaatttcc tgccaaaatc gacgtttttc ggcgaaaatt ggtagatttt gaaccgaaaa
     2941 ttgtgaaaaa tggacttttt tgctcaaaaa cagacttttt gagctttttt cacactgaaa
     3001 atggtggaaa attggatttt ccagcagaaa attcggaaat tttcaatttt tggcggaaat
     3061 ttgacgaaaa atatatgaaa aacaccgatt tttgtctgaa aaagttcaaa agccgaaaaa
     3121 ttggccaaaa atctccaaaa tcggactttt ctggcgtttt tcgtggaaaa attgaaattt
     3181 ttttgaaaaa ttgagcaaaa atcgccgaaa aaccgctaaa aaaactgccg aaattggcaa
     3241 ttttgagctt aaaaaaactt gaaaaatgct caaaattacc caattttcac cggaaattga
     3301 gctaaaatcg tcgaaaaaga ctgaattttc aatttttagc tgaaaatctg cctattttcg
     3361 gctaaaattg ctttaaaaac attaaaattc aaattttttg gctaaaaata ctcagattgc
     3421 cctttttttt ctccaaaaat gacaaaaaaa aaatcaatca gaaaaatgcg atttttttta
     3481 caaaaattgg cttcaaaacg tctaaattct ccctaatttt tgctgctgaa aattgtgcaa
     3541 aaatcaccga aaaatgacga ttttaggctg aaaatcttca aaaaaagctc aaaattcccc
     3601 acttttcacc ggaaaaagac gaaaaaagct ccaaatttat cgattaacat tttcagACCC
     3661 GTGCCGAGGT CAAGTTCGAG GGAGACACCC TCGTCAACCG TATCGAGCTC AAGGGAATCG
     3721 ACTTCAAGGA GGACGGTAAC ATCCTGGGAC ACAAGCTGGA GTACAACTAC AACTCCCACA
     3781 ACGTCTACAT CATGGCCGAC AAGCAAAAGA ACGGAATCAA GGTCAACTTC AAGgtaagtt
     3841 cttttttttg ctcgtttttg agtgaaaaat tgatggaatt cacaaaaaat ggccaaagaa
     3901 tcctcaaaat tggccgaaaa ttctgaaaaa tgacctaaaa atcaccgaaa aacggccaaa
     3961 aattatctga aaatcgccga aaatctcaaa ttttgaggaa attcccgcta aaaattaata
     4021 aaaatcaccg aaaaaaggcc aaaaattacc taataaaggc cgaaaatcgc ctaaaaaggc
     4081 caaaaattct gaaaaatggc caaatttcaa tttttcacac acaaaaaatc cctgaatttt
     4141 ctatttttac tcgaaaaact gtggatttac gggggtaaaa attcagtttt tagtgctatt
     4201 tcacaagaaa aacacagcat tttcgacaaa aactccgatt tttaagccaa aaatttgagg
     4261 tttttagttt tttacgtgaa aacctgaact ttttgccaaa aaaacacttt tttttagact
     4321 aaaaatcaat tttcgatcaa aaatcactaa ttttgcctga aaaaatatag atttttccac
     4381 tttttcactg aaaaaaaact ggattttcac tagaaaatca agttgttttt agaccaaaaa
     4441 ttttattttt ccgctgaaaa ttatcgtttt tcagtcaatt ttcagctaaa aatccctgaa
     4501 attctggatt ttcagccaaa aaactcgaaa ttttccaaaa aaaccccgtt taaaaatgag
     4561 tttagactga aaattccgat ttttgagcca aaaatttgat ttttaatcga aaaaaattgg
     4621 attttcaacc aaaaaatcag tttttcagtg atttttagag taaaaatcac tgattttaac
     4681 acaaaattcc agtttttcgt gatttttcac tagaaaatcc cgatttttcc ccaaaaaatc
     4741 catttttccg actaaaaatc cccaaagtaa ttttcagATC CGTCACAACA TCGAGGACGG
     4801 ATCGGTCCAA CTCGCCGACC ACTACCAACA AAACACCCCA ATCGGTGACG GACCAGTCCT
     4861 CCTCCCAGAC AACCACTACC TCTCCACCCA ATCCGCCCTC TCCAAGGACC CAAACGAGAA
     4921 GCGTGACCAC ATGGTCCTCC TCGAGTTCGT CACCGCCGCC GGAATCACCC ACGGAATGGA
     4981 CGAGCTCTAC AAGTCCCGTC GTCGTAAGGC CAACCCAACC AAGCTCTCCG AGAACGCCAA
     5041 GAAGCTCGCC AAGGAGGTCG AGAACGGATC CAACCCAGCT TTCTTGTACA AAGTGGGATA
     5101 Aatgcaaaat cctttcaagc attcccttct tctctatcac tcttctttct ttttgtcaaa
     5161 aaattctctc gctaatttat ttgctttttt aatgttatta ttttatgact ttttatagtc
     5221 actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa atgtgattct gtctgatgta
     5281 ctttcacaat ctctcttcaa ttccattttg aagtgcttta aacccgaaag gttgagaaaa
     5341 atgcgagcgc tcaaatattt gtattgtgtt cgttgagtga cccaacaaaa agaggaaact
     5401 ttattgtgcc gccaagaaaa aagtctcaCA ACTTTattat acatagttga taattcCCTC
     5461 TGACACATGC AGCTCgagct caggaaacgg agaatctgtg cggcaatgtc ttatcatttg
     5521 aaacgcgccg tatcgatgct gacatcatgc ggctcccgtg ttagtcgaga agatgggtac
     5581 gggcctttat tcatccaaca ctctacggct tcttcaagag ctagtagggc accctgtagt
     5641 tggaaaggga ttatttcgta tggcgagccc ataccgtctt tcttgcggaa gcttaacacg
     5701 ataggaagtt ggatagtttc gaacgatggt tccatacgga acgctgtctc aaggatgagt
     5761 ctgacggagt gtaactcgaa ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
     5821 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
     5881 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt
     5941 atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6001 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6061 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6121 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6181 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6241 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6301 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6361 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6421 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6481 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6541 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6601 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa
     6661 gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc ctcgtgatac
     6721 caattcggag cctgcttttt tgtacaaact tgttgataat ggcaattcaa ggatcttcac
     6781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6961 accatctggc cccagtgctg caatgatacc gcgagagcca cgctcaccgg ctccagattt
     7021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7801 acaaataggg gttccgcgca catttccccg aaaagtgcca gatacctgaa acaaaaccca
     7861 tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca
     7921 gtcacgacgt t    
//
