LOCUS       pCFJ1985                5205 bp    DNA        circular     14-DEC-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             join(2403..2417,2462..2699,3005..3153,3476..3673,
                     4205..4294)
                     /locus_tag="tagBFP2"
                     /label="tagBFP2"
                     /ApEinfo_label="tagBFP2"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(4940..5035)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2367..2402
                     /locus_tag="Flexible linker 1B"
                     /label="Flexible linker 1B"
                     /ApEinfo_label="Flexible linker 1B"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4295..4339
                     /locus_tag="Flexible linker 2B"
                     /label="Flexible linker 2B"
                     /ApEinfo_label="Flexible linker 2B"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="#0080ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     join(2284..2301,2358..2360)
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3154..3475
                     /locus_tag="Intron w PATCs"
                     /label="Intron w PATCs"
                     /ApEinfo_label="Intron w PATCs"
                     /ApEinfo_fwdcolor="#b3b3b3"
                     /ApEinfo_revcolor="#b3b3b3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3674..4204
                     /locus_tag="Intron w PATCs(1)"
                     /label="Intron w PATCs(1)"
                     /ApEinfo_label="Intron w PATCs"
                     /ApEinfo_fwdcolor="#b3b3b3"
                     /ApEinfo_revcolor="#b3b3b3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          2700..3004
                     /locus_tag="intron w PATCs"
                     /label="intron w PATCs"
                     /ApEinfo_label="intron w PATCs"
                     /ApEinfo_fwdcolor="#b3b3b3"
                     /ApEinfo_revcolor="#b3b3b3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          4361..4872
                     /locus_tag="intron w PATCs(1)"
                     /label="intron w PATCs(1)"
                     /ApEinfo_label="intron w PATCs"
                     /ApEinfo_fwdcolor="#b3b3b3"
                     /ApEinfo_revcolor="#b3b3b3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          2302..2357
                     /locus_tag="intron w PATCs(2)"
                     /label="intron w PATCs(2)"
                     /ApEinfo_label="intron w PATCs"
                     /ApEinfo_fwdcolor="#b3b3b3"
                     /ApEinfo_revcolor="#b3b3b3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          2418..2461
                     /locus_tag="intron w PATCs(3)"
                     /label="intron w PATCs(3)"
                     /ApEinfo_label="intron w PATCs"
                     /ApEinfo_fwdcolor="#b3b3b3"
                     /ApEinfo_revcolor="#b3b3b3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2278..2283
                     /locus_tag="BamHI site"
                     /label="BamHI site"
                     /ApEinfo_label="BamHI site"
                     /ApEinfo_fwdcolor="#333333"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4340..4345
                     /locus_tag="NaeI site"
                     /label="NaeI site"
                     /ApEinfo_label="NaeI site"
                     /ApEinfo_fwdcolor="#333333"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4930..4935
                     /locus_tag="BamHI site(1)"
                     /label="BamHI site(1)"
                     /ApEinfo_label="BamHI site"
                     /ApEinfo_fwdcolor="#333333"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide join(4346..4360,4873..4929)
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2361..2366
                     /locus_tag="NaeI site(1)"
                     /label="NaeI site(1)"
                     /ApEinfo_label="NaeI site"
                     /ApEinfo_fwdcolor="#333333"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     2177..2272
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2143..2160
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(5073..5093)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(5053..5074)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1..807
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    957..1545
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT GGATGCTGAT
       61 TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGC
      121 TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC
      181 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT GCCTCTTCCG
      241 ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC TGCGATCCCC
      301 GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA TATTGTTGAT
      361 GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC
      421 AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT
      481 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG GAAAGAAATG
      541 CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT CTCACTTGAT
      601 AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG AGTCGGAATC
      661 GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA
      721 TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG
      781 TTTCATTTGA TGCTCGATGA GTTTTTCTAA tcagaattgg ttaattggtt gtaacactgg
      841 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
      901 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1621 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1681 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1741 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1801 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     1861 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     1921 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     1981 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     2041 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     2101 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     2161 cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
     2221 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctcgGGA
     2281 TCCCCAAAGA AGAAGCGTAA Ggtaagtttt gaaatattca tatttttaac ttgaaaacta
     2341 atatttaaat tttttagGTC GCCGGCGGAT CATCCTCCAC ATCATCCTCC ACATCATCCT
     2401 CATCCGAGCT CATCAAGgta acacttagtt tttgttgctc tgtttaaaaa ttaattttaa
     2461 gGAGAACATG CACATGAAGC TCTACATGGA GGGAACCGTC GACAACCACC ACTTCAAGTG
     2521 CACCTCCGAG GGAGAGGGAA AGCCATACGA GGGAACCCAA ACCATGCGTA TCAAGGTCGT
     2581 CGAGGGAGGA CCACTCCCAT TCGCCTTCGA CATCCTCGCC ACCTCCTTCC TCTACGGATC
     2641 GAAGACCTTC ATCAACCACA CCCAAGGAAT CCCAGACTTC TTCAAGCAAT CCTTCCCAGg
     2701 taagttttct tatgggaaag aaggaaaaaa ccgagatttt acttgaaaaa ttgaattttt
     2761 cgcgggattt tcaccaaaaa ttgttgaata ttcattattt cacgctgtaa aacaaaaaaa
     2821 aaaaaaatca aaaactacgt tgaaatcgcg tttttaagcg aattttcttc agaattgcca
     2881 gattttaacc ccaaattttg cagtttttaa ataaaatttc accttttcgg ctcaaattgt
     2941 agattttcct gaaaatttag tacaaaaaac aatttcctcg taaatttttc aaatagattt
     3001 tcagAGGGAT TCACCTGGGA GCGTGTCACC ACCTACGAGG ACGGAGGAGT CCTCACCGCC
     3061 ACCCAAGACA CCTCCCTCCA AGACGGATGC CTCATCTACA ACGTCAAGAT CCGTGGAGTC
     3121 AACTTCACCT CCAACGGACC AGTCATGCAA AAGgtaagtt atgtctacct gcctgcctac
     3181 cgcctaaatt ttttgtgaag tttcttcaaa aaatccagaa aaaaaaacaa ttttcatacg
     3241 attttttccc ttaaaattgt gaattttcat gctttttagc cccaaaagtc attatttgag
     3301 aaaaaatttc atacaaaaaa gttttgagaa atacacaatt ttttaaatgt aattttcaaa
     3361 ttttcaattt tcaactagaa aattcacaaa acttgtaaat tttggaccaa aacatttata
     3421 caattacttt ttttgaatct aataactaca ataactacaa atttaacatt ttcagAAGAC
     3481 CCTCGGATGG GAGGCCTTCA CCGAGACGCT CTACCCAGCC GACGGAGGAC TGGAGGGACG
     3541 TAACGACATG GCCCTCAAGC TCGTCGGAGG ATCGCACCTC ATCGCCAACG CCAAGACCAC
     3601 CTACCGTTCC AAGAAGCCAG CCAAGAACCT CAAGATGCCA GTCTACTACG TCGACTACCG
     3661 TCTGGAGCGT AAGgtaagtt ctttttttga aaagtgcagt ttgtagtcta attttcattt
     3721 attttctttt taaaaaacgc atcaattttt aaatattttg ggacaaaatc cgaaaactgt
     3781 actaatttgt agtttgtaaa attaaaaaaa aaacgcaaaa aaatgttttc aaaaatgctt
     3841 agaataaaaa taaaatttct aaaaaattga acaaaataaa catttaaaaa ttcaaaaagt
     3901 ttgaaaaaat gcaaagtttt ttaatgcaaa aaatatttaa tgtttaaaca aatttaaaaa
     3961 atgttgtaag aatatgttta gagacagttt ttaaatgtta aattgcaaaa aaaaaaagca
     4021 aaaaaaaatt tgaaaaatgt aacagaaaat tttaattaaa aaagtcgtgt ttttttcaaa
     4081 cctgcaattc tttgtaattt tatttataaa gagaaacaaa tcaaattgta tattacgaaa
     4141 aacgatacta aaattcgaaa atttgcggtt ttttgcgtaa aaaatacggt tccgtaattt
     4201 tcagGAGGCC AACAACGAGA CGTACGTCGA GCAACACGAG GTCGCCGTCG CCCGTTACTG
     4261 CGACCTCCCA TCCAAGCTCG GACACAAGCT CAACTCGGGA ACTTCTACGA CTTCGGTAAC
     4321 GTCATCCGGT ACTTCCCCAG CCGGCTCCCG TCGTCGTAAG gtaagttcat agattttgaa
     4381 aaaaagttta agaactgaaa aatggaataa aaatatttaa gagcattttt aaatgtaaaa
     4441 ttaacaaaaa aagcgcctaa gaatgtttcc aaaacagtaa aaaaaaggtt taaaaaaatg
     4501 caaaaaaaaa tttaagatgc ttttaattac acaaaaaatg aaagtaaaaa aaattaaaat
     4561 attgaaaaat gtatatttgt ttgaaaaggt acatttttca atgcaaaaaa tgtttctaaa
     4621 aattaaaaaa atatgtttaa attgtgtaaa gtgtaaatta aaaaataaaa ttatgaaaaa
     4681 gtattgtttc atactttttc taaattttgg ttgaaaaatt taaaattcca gctttttatt
     4741 cacattatgt gttctaaaaa tctttcccca attttcggga ccgttttcca gtatatttcc
     4801 atttttcaaa aagattttta actgaaattc atgttttcaa tgctaaaaat caataaaaaa
     4861 agaatttttc agGCCAACCC AACCAAGCTC TCCGAGAACG CCAAGAAGCT CGCCAAGGAG
     4921 GTCGAGAACG GATCCAACCC AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC
     4981 TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT
     5041 CCAGCTGATA TCCCCTATAG TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC
     5101 TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA
     5161 ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTT 
//
