LOCUS       pCFJ2249____1_2_        3545 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    677..715
                     /locus_tag="flex linker"
                     /label="flex linker"
                     /ApEinfo_label="flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1624..1662
                     /locus_tag="Flex linker"
                     /label="Flex linker"
                     /ApEinfo_label="Flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  671..676
                     /locus_tag="NaeI"
                     /label="NaeI"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  1663..1668
                     /locus_tag="NaeI(1)"
                     /label="NaeI(1)"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(716..721,766..982,1034..1128,1180..1356,1408..1623)
                     /locus_tag="GFP"
                     /label="GFP"
                     /ApEinfo_label="GFP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..665
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1939..2745
                     /locus_tag="Kan (no BsmBI)"
                     /label="Kan (no BsmBI)"
                     /ApEinfo_label="Kan (no BsmBI)"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(1673..1768)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1806..1826)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1786..1807)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2895..3483
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg ataagcaatg ctttcttata atgccaactt tgtacaaaaa
      661 agcaggcTCG GCCGGCAGTA CTAGCGGTGG CAGTGGAGGT ACCGGCGGAA GCAGTTCCAA
      721 Ggtaacactt agtttttgtt gctctgttta aaaattaatt ttaagGGAGA GGAGCTGTTC
      781 ACCGGAGTCG TCCCAATCCT CGTCGAGCTC GACGGGGACG TCAACGGACA CAAGTTCTCC
      841 GTGTCCGGAG AGGGAGAGGG AGATGCCACC TACGGAAAGC TCACCCTCAA GTTCATCTGC
      901 ACCACCGGAA AGCTCCCAGT CCCATGGCCA ACCCTCGTCA CCACCTTCTG CTACGGAGTC
      961 CAATGCTTCT CCCGTTACCC AGgtaagttt tcttgagacc tttactaact aactaggtct
     1021 catagatttt cagACCACAT GAAGCGTCAC GACTTCTTCA AGTCCGCCAT GCCAGAGGGA
     1081 TACGTCCAAG AGCGTACCAT CTTCTTCAAG GACGACGGAA ACTACAAGgt aagttatgta
     1141 gagaccttta ctaactaact aggtctcaaa cattttcagA CCCGTGCCGA GGTCAAGTTC
     1201 GAGGGAGACA CCCTCGTCAA CCGTATCGAG CTCAAGGGAA TCGACTTCAA GGAGGACGGT
     1261 AACATCCTGG GACACAAGCT GGAGTACAAC TACAACTCCC ACAACGTCTA CATCATGGCC
     1321 GACAAGCAAA AGAACGGAAT CAAGGTCAAC TTCAAGgtaa gttctttaga gacctttact
     1381 aactaactag gtctcagtaa ttttcagATC CGTCACAACA TCGAGGACGG ATCGGTCCAA
     1441 CTCGCCGACC ACTACCAACA AAACACCCCA ATCGGTGACG GACCAGTCCT CCTCCCAGAC
     1501 AACCACTACC TCTCCACCCA ATCCGCCCTC TCCAAGGACC CAAACGAGAA GCGTGACCAC
     1561 ATGGTCCTCC TCGAGTTCGT CACCGCCGCC GGAATCACCC ACGGAATGGA CGAGCTCTAC
     1621 AAGGGTGGCA CAGGAGGAAC GGGCGGTAGT GGAGGCACTG GTGCCGGCaa cccagctttc
     1681 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1741 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1801 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1861 tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc ttacataaac
     1921 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     1981 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2041 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2101 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2161 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2221 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2281 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2341 tgtaattgtc cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca atcacgaatg
     2401 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2461 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2521 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2581 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2641 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2701 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2761 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     2821 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     2881 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2941 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3001 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3061 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3121 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3181 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3241 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3301 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3361 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3421 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3481 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3541 atgtt     
//
