LOCUS       pCFJ2306____1_2_        3560 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_structure  671..676
                     /locus_tag="BamHI"
                     /label="BamHI"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(698..703,748..964,1016..1110,1162..1338,1390..1605)
                     /locus_tag="GFP"
                     /label="GFP"
                     /ApEinfo_label="GFP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..665
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  1678..1683
                     /locus_tag="BamHI(1)"
                     /label="BamHI(1)"
                     /ApEinfo_label="BamHI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(1688..1783)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     sig_peptide     677..697
                     /locus_tag="SV40 (no BsaI)"
                     /label="SV40 (no BsaI)"
                     /ApEinfo_label="SV40 (no BsaI)"
                     /ApEinfo_fwdcolor="#f6006c"
                     /ApEinfo_revcolor="#f6006c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     transit_peptide 1606..1677
                     /locus_tag="egl-13 NLS"
                     /label="egl-13 NLS"
                     /ApEinfo_label="egl-13 NLS"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1821..1841)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1801..1822)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1954..2760
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2910..3498
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcg GGATCCCCAA AGAAGAAGCG TAAGGTGTCC AAGgtaacac ttagtttttg
      721 ttgctctgtt taaaaattaa ttttaagGGA GAGGAGCTGT TCACCGGAGT CGTCCCAATC
      781 CTCGTCGAGC TCGACGGGGA CGTCAACGGA CACAAGTTCT CCGTGTCCGG AGAGGGAGAG
      841 GGAGATGCCA CCTACGGAAA GCTCACCCTC AAGTTCATCT GCACCACCGG AAAGCTCCCA
      901 GTCCCATGGC CAACCCTCGT CACCACCTTC TGCTACGGAG TCCAATGCTT CTCCCGTTAC
      961 CCAGgtaagt tttcttgaga cctttactaa ctaactaggt ctcatagatt ttcagACCAC
     1021 ATGAAGCGTC ACGACTTCTT CAAGTCCGCC ATGCCAGAGG GATACGTCCA AGAGCGTACC
     1081 ATCTTCTTCA AGGACGACGG AAACTACAAG gtaagttatg tagagacctt tactaactaa
     1141 ctaggtctca aacattttca gACCCGTGCC GAGGTCAAGT TCGAGGGAGA CACCCTCGTC
     1201 AACCGTATCG AGCTCAAGGG AATCGACTTC AAGGAGGACG GTAACATCCT GGGACACAAG
     1261 CTGGAGTACA ACTACAACTC CCACAACGTC TACATCATGG CCGACAAGCA AAAGAACGGA
     1321 ATCAAGGTCA ACTTCAAGgt aagttcttta gagaccttta ctaactaact aggtctcagt
     1381 aattttcagA TCCGTCACAA CATCGAGGAC GGATCGGTCC AACTCGCCGA CCACTACCAA
     1441 CAAAACACCC CAATCGGTGA CGGACCAGTC CTCCTCCCAG ACAACCACTA CCTCTCCACC
     1501 CAATCCGCCC TCTCCAAGGA CCCAAACGAG AAGCGTGACC ACATGGTCCT CCTCGAGTTC
     1561 GTCACCGCCG CCGGAATCAC CCACGGAATG GACGAGCTCT ACAAGTCCCG TCGTCGTAAG
     1621 GCCAACCCAA CCAAGCTCTC CGAGAACGCC AAGAAGCTCG CCAAGGAGGT CGAGAACGGA
     1681 TCCaacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1741 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1801 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1861 caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac aataaaactg
     1921 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1981 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     2041 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     2101 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2161 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2221 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     2281 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2341 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2401 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2461 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2521 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2581 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2641 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2701 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2761 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2821 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2881 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2941 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3001 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3061 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3121 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3181 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3241 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3301 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3361 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3421 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3481 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3541 tggccttttg ctcacatgtt    
//
