LOCUS       pCFJ2440                6218 bp    DNA        circular     14-DEC-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    677..715
                     /locus_tag="flex linker"
                     /label="flex linker"
                     /ApEinfo_label="flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  671..676
                     /locus_tag="NaeI"
                     /label="NaeI"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4297..4335
                     /locus_tag="Flex linker"
                     /label="Flex linker"
                     /ApEinfo_label="Flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  4336..4341
                     /locus_tag="NaeI(1)"
                     /label="NaeI(1)"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(716..721,766..982,1892..1986,2960..3136,4081..4296)
                     /locus_tag="ceGFP"
                     /label="ceGFP"
                     /ApEinfo_label="ceGFP"
                     /ApEinfo_fwdcolor="#00ff80"
                     /ApEinfo_revcolor="#00ff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..665
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4612..5418
                     /locus_tag="Kan (no BsmBI)"
                     /label="Kan (no BsmBI)"
                     /ApEinfo_label="Kan (no BsmBI)"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(4346..4441)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(4479..4499)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(4459..4480)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5568..6156
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg ataagcaatg ctttcttata atgccaactt tgtacaaaaa
      661 agcagGCTCG GCCGGCAGTA CTAGCGGTGG CAGTGGAGGT ACCGGCGGAA GCAGTTCCAA
      721 Ggtaacactt agtttttgtt gctctgttta aaaattaatt ttaagGGAGA GGAGCTGTTC
      781 ACCGGAGTCG TCCCAATCCT CGTCGAGCTC GACGGGGACG TCAACGGACA CAAGTTCTCC
      841 GTGTCCGGAG AGGGAGAGGG AGATGCCACC TACGGAAAGC TCACCCTCAA GTTCATCTGC
      901 ACCACCGGAA AGCTCCCAGT CCCATGGCCA ACCCTCGTCA CCACCTTCTG CTACGGAGTC
      961 CAATGCTTCT CCCGTTACCC AGgtaagttt tctgttttat ttacttgttt tgagaattgg
     1021 gatttttagc tgaaaaattg gattttaaga tgaaaaattg aaattttcaa ctaaaaattt
     1081 caatttttag ctgaaaagtt aaatttttag ctaaagagtg ggatttttag ttgaaaaatc
     1141 gagatttcta gctaaaaagt atgattttta gctgaaagat tggaatttta agcagaaaaa
     1201 attgtatttt tagctgataa atcaaatttt aagcggaaaa atttgaattt ttagctaaaa
     1261 aattccattt tcaactaaaa atcgtgattt ttagctttaa aaattggatt tttagctgaa
     1321 aaatcggatt tttagctgaa aattgaaatt ttttagttaa aaagtgagat tttttaacag
     1381 aaaagtgaga tttttagtta aaaaataata tttttagcta aaaaatacaa ttttcaacta
     1441 aagatcgtga tttttagctt taaaaattgg atttttagct aaaaaatcgg atttttagct
     1501 gaaaattgaa attttttagt taaaaagtgg gattttaaga tgaaaagtga gattttttag
     1561 ctgaaaagtg agattttcag ctaaaaaata caattttcaa ctgaaaaatt gggattttta
     1621 gctttaaaaa ttggattttc agctttaaaa attggatttt tagctgaaaa gtgagatttt
     1681 tagttgaaaa attgaaatgt ttagctgaaa aattggaatt tttaagtaaa aaagtgggat
     1741 tttaagatga aaagtgagat gtttttagct gaaaagtgag atttttagct aaaaaataca
     1801 attttcaact gaaaaattgg gatttttagc tcaaaagtgg gacgaaagac gaaaaaaaaa
     1861 atcgattttt taccaaaaat tagattttca gACCACATGA AGCGTCACGA CTTCTTCAAG
     1921 TCCGCCATGC CAGAGGGATA CGTCCAAGAG CGTACCATCT TCTTCAAGGA CGACGGAAAC
     1981 TACAAGgtaa gttatgtacg cgatttctcg gcgaaaaaat ggactttttt gctcaaaaac
     2041 tgaatttttc agcttttttt gcactgaaaa tgctgaaaat tggttttttt gctgaaaatt
     2101 caccgaaaaa agctgaaaat attgcagaaa tcgacgaaaa atgccttttt tgctgaaatt
     2161 tcagccagaa aattgttcag aatcgcaatt tcctgccaaa atcgacgttt ttcggcgaaa
     2221 attggtagat tttgaaccga aaattgtgaa aaatggactt ttttgctcaa aaacagactt
     2281 tttgagcttt tttcacactg aaaatggtgg aaaattggat tttccagcag aaaattcgga
     2341 aattttcaat ttttggcgga aatttgacga aaaatatatg aaaaacaccg atttttgtct
     2401 gaaaaagttc aaaagccgaa aaattggcca aaaatctcca aaatcggact tttctggcgt
     2461 ttttcgtgga aaaattgaaa tttttttgaa aaattgagca aaaatcgccg aaaaaccgct
     2521 aaaaaaactg ccgaaattgg caattttgag cttaaaaaaa cttgaaaaat gctcaaaatt
     2581 acccaatttt caccggaaat tgagctaaaa tcgtcgaaaa agactgaatt ttcaattttt
     2641 agctgaaaat ctgcctattt tcggctaaaa ttgctttaaa aacattaaaa ttcaaatttt
     2701 ttggctaaaa atactcagat tgcccttttt tttctccaaa aatgacaaaa aaaaaatcaa
     2761 tcagaaaaat gcgatttttt ttacaaaaat tggcttcaaa acgtctaaat tctccctaat
     2821 ttttgctgct gaaaattgtg caaaaatcac cgaaaaatga cgattttagg ctgaaaatct
     2881 tcaaaaaaag ctcaaaattc cccacttttc accggaaaaa gacgaaaaaa gctccaaatt
     2941 tatcgattaa cattttcagA CCCGTGCCGA GGTCAAGTTC GAGGGAGACA CCCTCGTCAA
     3001 CCGTATCGAG CTCAAGGGAA TCGACTTCAA GGAGGACGGT AACATCCTGG GACACAAGCT
     3061 GGAGTACAAC TACAACTCCC ACAACGTCTA CATCATGGCC GACAAGCAAA AGAACGGAAT
     3121 CAAGGTCAAC TTCAAGgtaa gttctttttt ttgctcgttt ttgagtgaaa aattgatgga
     3181 attcacaaaa aatggccaaa gaatcctcaa aattggccga aaattctgaa aaatgaccta
     3241 aaaatcaccg aaaaacggcc aaaaattatc tgaaaatcgc cgaaaatctc aaattttgag
     3301 gaaattcccg ctaaaaatta ataaaaatca ccgaaaaaag gccaaaaatt acctaataaa
     3361 ggccgaaaat cgcctaaaaa ggccaaaaat tctgaaaaat ggccaaattt caatttttca
     3421 cacacaaaaa atccctgaat tttctatttt tactcgaaaa actgtggatt tacgggggta
     3481 aaaattcagt ttttagtgct atttcacaag aaaaacacag cattttcgac aaaaactccg
     3541 atttttaagc caaaaatttg aggtttttag ttttttacgt gaaaacctga actttttgcc
     3601 aaaaaaacac ttttttttag actaaaaatc aattttcgat caaaaatcac taattttgcc
     3661 tgaaaaaata tagatttttc cactttttca ctgaaaaaaa actggatttt cactagaaaa
     3721 tcaagttgtt tttagaccaa aaattttatt tttccgctga aaattatcgt ttttcagtca
     3781 attttcagct aaaaatccct gaaattctgg attttcagcc aaaaaactcg aaattttcca
     3841 aaaaaacccc gtttaaaaat gagtttagac tgaaaattcc gatttttgag ccaaaaattt
     3901 gatttttaat cgaaaaaaat tggattttca accaaaaaat cagtttttca gtgattttta
     3961 gagtaaaaat cactgatttt aacacaaaat tccagttttt cgtgattttt cactagaaaa
     4021 tcccgatttt tccccaaaaa atccattttt ccgactaaaa atccccaaag taattttcag
     4081 ATCCGTCACA ACATCGAGGA CGGATCGGTC CAACTCGCCG ACCACTACCA ACAAAACACC
     4141 CCAATCGGTG ACGGACCAGT CCTCCTCCCA GACAACCACT ACCTCTCCAC CCAATCCGCC
     4201 CTCTCCAAGG ACCCAAACGA GAAGCGTGAC CACATGGTCC TCCTCGAGTT CGTCACCGCC
     4261 GCCGGAATCA CCCACGGAAT GGACGAGCTC TACAAGGGTG GCACAGGAGG AACGGGCGGT
     4321 AGTGGAGGCA CTGGTGCCGG Caacccagct ttcttgtaca aagttggcat tataagaaag
     4381 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     4441 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     4501 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aataatatca
     4561 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     4621 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     4681 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     4741 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     4801 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     4861 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     4921 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     4981 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     5041 gtatttcgcc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     5101 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     5161 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     5221 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     5281 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     5341 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     5401 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     5461 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     5521 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5581 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5641 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5701 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5761 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5821 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5881 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5941 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6001 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6061 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6121 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6181 cggttcctgg ccttttgctg gccttttgct cacatgtt  
//
