LOCUS       pCFJ2438____1_2_        6234 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    677..715
                     /locus_tag="flex linker"
                     /label="flex linker"
                     /ApEinfo_label="flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  671..676
                     /locus_tag="NaeI"
                     /label="NaeI"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4495..4515)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..665
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4475..4496)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4313..4351
                     /locus_tag="Flex linker"
                     /label="Flex linker"
                     /ApEinfo_label="Flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4628..5434
                     /locus_tag="Kan (no BsmBI)"
                     /label="Kan (no BsmBI)"
                     /ApEinfo_label="Kan (no BsmBI)"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  4352..4357
                     /locus_tag="NaeI(1)"
                     /label="NaeI(1)"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(4362..4457)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(716..721,766..966,1877..2009,2983..3173,4118..4312)
                     /locus_tag="mCardinal"
                     /label="mCardinal"
                     /ApEinfo_label="mCardinal"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctCG GCCGGCAGTA CTAGCGGTGG CAGTGGAGGT ACCGGCGGAA GCAGTTCCAA
      721 Ggtaacactt agtttttgtt gctctgttta aaaattaatt ttaagGGAGA GGAGCTCATC
      781 AAGGAGAACA TGCACATGAA GCTCTACATG GAGGGAACCG TCAACAACCA CCACTTCAAG
      841 TGCACCACCG AGGGAGAGGG AAAGCCATAC GAGGGAACCC AAACCCAACG TATCAAGGTC
      901 GTCGAGGGAG GACCACTCCC ATTCGCCTTC GACATCCTCG CCACCTGCTT CATGTACGGA
      961 TCGAAGgtaa gttttctgtt ttatttactt gtttctgaga attgggattt ttagctgaaa
     1021 aattggattt taagatgaaa aattgaaatt ttcaactaaa aatttcaatt tttagctgaa
     1081 aagttaaatt tttagctaaa gagtgggatt tttagttgaa aaatcgagat ttctagctaa
     1141 aaagtatgat ttttagctga aagattggaa ttttaagcag aaaaaattgt atttttagct
     1201 gataaatcaa attttaagcg gaaaaatttg aatttttagc taaaaaattc cattttcaac
     1261 taaaaatcgt gatttttagc tttaaaaatt ggatttttag ctgaaaaatc ggatttttag
     1321 ctgaaaattg aaatttttta gttaaaaagt gagatttttt aacagaaaag tgagattttt
     1381 agttaaaaaa taatattttt agctaaaaaa tacaattttc aactaaagat cgtgattttt
     1441 agctttaaaa attggatttt tagctaaaaa atcggatttt tagctgaaaa ttgaaatttt
     1501 ttagttaaaa agtgggattt taagatgaaa agtgagattt tttagctgaa aagtgagatt
     1561 ttcagctaaa aaatacaatt ttcaactgaa aaattgggat ttttagcttt aaaaattgga
     1621 ttttcagctt taaaaattgg atttttagct gaaaagtgag atttttagtt gaaaaattga
     1681 aatgtttagc tgaaaaattg gaatttttaa gtaaaaaagt gggattttaa gatgaaaagt
     1741 gagatgtttt tagctgaaaa gtgagatttt tagctaaaaa atacaatttt caactgaaaa
     1801 attgggattt ttagctcaaa agtgggacga aagacgaaaa aaaaaatcga ttttttacca
     1861 aaaattagat tttcagACCT TCATCAACCA CACCCAAGGA ATCCCAGACT TCTTCAAGCA
     1921 ATCCTTCCCA GAGGGATTCA CCTGGGAGCG TGTCACCACC TACGAGGACG GAGGAGTCCT
     1981 CACCGTCACC CAAGACACCT CCCTCCAAGg taagttatgt acgcgatttc tcggcgaaaa
     2041 aatggacttt tttgctcaaa aactgaattt ttcagctttt tttgcactga aaatgctgaa
     2101 aattggtttt tttgctgaaa attcaccgaa aaaagctgaa aatattgcag aaatcgacga
     2161 aaaatgcctt ttttgctgaa atttcagcca gaaaattgtt cagaatcgca atttcctgcc
     2221 aaaatcgacg tttttcggcg aaaattggta gattttgaac cgaaaattgt gaaaaatgga
     2281 cttttttgct caaaaacaga ctttttgagc ttttttcaca ctgaaaatgg tggaaaattg
     2341 gattttccag cagaaaattc ggaaattttc aatttttggc ggaaatttga cgaaaaatat
     2401 atgaaaaaca ccgatttttg tctgaaaaag ttcaaaagcc gaaaaattgg ccaaaaatct
     2461 ccaaaatcgg acttttctgg cgtttttcgt ggaaaaattg aaattttttt gaaaaattga
     2521 gcaaaaatcg ccgaaaaacc gctaaaaaaa ctgccgaaat tggcaatttt gagcttaaaa
     2581 aaacttgaaa aatgctcaaa attacccaat tttcaccgga aattgagcta aaatcgtcga
     2641 aaaagactga attttcaatt tttagctgaa aatctgccta ttttcggcta aaattgcttt
     2701 aaaaacatta aaattcaaat tttttggcta aaaatactca gattgccctt ttttttctcc
     2761 aaaaatgaca aaaaaaaaat caatcagaaa aatgcgattt tttttacaaa aattggcttc
     2821 aaaacgtcta aattctccct aatttttgct gctgaaaatt gtgcaaaaat caccgaaaaa
     2881 tgacgatttt aggctgaaaa tcttcaaaaa aagctcaaaa ttccccactt ttcaccggaa
     2941 aaagacgaaa aaagctccaa atttatcgat taacattttc agACGGATGC CTCATCTACA
     3001 ACGTCAAGCT CCGTGGAGTC AACTTCCCAT CCAACGGACC AGTCATGCAA AAGAAGACCC
     3061 TCGGATGGGA GGCCACCACC GAGACGCTCT ACCCAGCCGA CGGAGGACTC GAGGGACGTT
     3121 GCGACATGGC CCTCAAGCTC GTCGGAGGAG GACACCTCCA CTGCAACCTC AAGgtaagtt
     3181 cttttttttg ctcgtttttg agtgaaaaat tgatggaatt cacaaaaaat ggccaaagaa
     3241 tcctcaaaat tggccgaaaa ttctgaaaaa tgacctaaaa atcaccgaaa aacggccaaa
     3301 aattatctga aaatcgccga aaatctcaaa ttttgaggaa attcccgcta aaaattaata
     3361 aaaatcaccg aaaaaaggcc aaaaattacc taataaaggc cgaaaatcgc ctaaaaaggc
     3421 caaaaattct gaaaaatggc caaatttcaa tttttcacac acaaaaaatc cctgaatttt
     3481 ctatttttac tcgaaaaact gtggatttac gggggtaaaa attcagtttt tagtgctatt
     3541 tcacaagaaa aacacagcat tttcgacaaa aactccgatt tttaagccaa aaatttgagg
     3601 tttttagttt tttacgtgaa aacctgaact ttttgccaaa aaaacacttt tttttagact
     3661 aaaaatcaat tttcgatcaa aaatcactaa ttttgcctga aaaaatatag atttttccac
     3721 tttttcactg aaaaaaaact ggattttcac tagaaaatca agttgttttt agaccaaaaa
     3781 ttttattttt ccgctgaaaa ttatcgtttt tcagtcaatt ttcagctaaa aatccctgaa
     3841 attctggatt ttcagccaaa aaactcgaaa ttttccaaaa aaaccccgtt taaaaatgag
     3901 tttagactga aaattccgat ttttgagcca aaaatttgat ttttaatcga aaaaaattgg
     3961 attttcaacc aaaaaatcag tttttcagtg atttttagag taaaaatcac tgattttaac
     4021 acaaaattcc agtttttcgt gatttttcac tagaaaatcc cgatttttcc ccaaaaaatc
     4081 catttttccg actaaaaatc cccaaagtaa ttttcagACC ACCTACCGTT CCAAGAAGCC
     4141 AGCCAAGAAC CTCAAGATGC CAGGAGTCTA CTTCGTCGAC CGTCGTCTCG AGCGTATCAA
     4201 GGAGGCCGAC AACGAGACGT ACGTCGAGCA ACACGAGGTC GCCGTCGCCC GTTACTGCGA
     4261 CCTCCCATCC AAGCTCGGAC ACAAGCTCAA CGGAATGGAC GAGCTCTACA AGGGTGGCAC
     4321 AGGAGGAACG GGCGGTAGTG GAGGCACTGG TGCCGGCAac ccagctttct tgtacaaagt
     4381 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     4441 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     4501 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     4561 agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     4621 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     4681 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     4741 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     4801 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     4861 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     4921 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     4981 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     5041 ttttaacagc gatcgcgtat ttcgcctcgc tcaggcgcaa tcacgaatga ataacggttt
     5101 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     5161 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     5221 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     5281 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     5341 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     5401 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     5461 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     5521 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5581 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5641 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5701 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5761 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5821 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5881 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5941 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6001 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6061 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6121 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6181 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
