LOCUS       pCFJ2210____1_2_        3542 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    677..715
                     /locus_tag="flex linker"
                     /label="flex linker"
                     /ApEinfo_label="flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1621..1659
                     /locus_tag="Flex linker"
                     /label="Flex linker"
                     /ApEinfo_label="Flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1936..2742
                     /locus_tag="Kan (no BsmBI)"
                     /label="Kan (no BsmBI)"
                     /ApEinfo_label="Kan (no BsmBI)"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  671..676
                     /locus_tag="NaeI"
                     /label="NaeI"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  1660..1665
                     /locus_tag="NaeI(1)"
                     /label="NaeI(1)"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(1670..1765)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..665
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(716..724,769..918,970..1087,1139..1320,1372..1620)
                     /locus_tag="mMaple3"
                     /label="mMaple3"
                     /ApEinfo_label="mMaple3"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          725..768
                     /locus_tag="intron rpl-8"
                     /label="intron rpl-8"
                     /ApEinfo_label="intron rpl-8"
                     /ApEinfo_fwdcolor="#bfbfbf"
                     /ApEinfo_revcolor="#bfbfbf"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1803..1823)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1783..1804)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2892..3480
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctCG GCCGGCAGTA CTAGCGGTGG CAGTGGAGGT ACCGGCGGAA GCAGTGTCTC
      721 CAAGgtaaca cttagttttt gttgctctgt ttaaaaatta attttaagGG AGAGGAGACT
      781 ATCATGTCCG TCATCAAGCC AGACATGAAG ATCAAGCTCC GTATGGAGGG AAACGTCAAC
      841 GGACACGCCT TCGTCATCGA GGGAGAGGGA TCCGGAAAGC CATTCGAGGG AATCCAAACC
      901 ATCGACCTCG AGGTCAAGgt aagttttctt gagaccttta ctaactaact aggtctcata
      961 gattttcagG AGGGAGCCCC ACTCCCATTC GCCTACGACA TCCTCACCAC CGCCTTCCAC
     1021 TACGGAAACC GTGTCTTCAC CAAGTACCCA CGAAAGATCC CAGACTACTT CAAGCAATCC
     1081 TTCCCAGgta agttatgtag agaccttaat actcaactat ggtctcaaac attttcagAG
     1141 GGATACTCCT GGGAGCGTTC CATGACCTAC GAGGACGGAG GAATCTGCAA CGCCACCAAC
     1201 GACATCACCA TGGAGGAGGA CTCCTTCATC AACAAGATCC ACTTCAAGGG AACCAACTTC
     1261 CCACCAAACG GACCAGTCAT GCAAAAGCGT ACCGTCGGAT GGGAGGTTTC CACCGAGAAG
     1321 gtaagttctt tagagaccta atcacatcaa tttggtctca gtaattttca gATGTACGTC
     1381 CGTGACGGAG TCCTCAAGGG TGACGTCAAG ATGAAGCTCC TCCTCAAGGG AGGATCCCAC
     1441 TACCGTTGCG ACTTCCGTAC CACCTACAAG GTCAAGCAAA AGGCCGTCAA GCTCCCAAAG
     1501 GCCCACTTCG TCGACCACCG TATCGAGATC CTCTCCCACG ACAAGGACTA CAACAAGGTC
     1561 AAGCTCTACG AGCACGCCGT CGCCCGTAAC TCCACCGACT CCATGGACGA GCTCTACAAG
     1621 GGTGGCACAG GAGGAACGGG CGGTAGTGGA GGCACTGGTG CCGGCAaccc agctttcttg
     1681 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1741 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1801 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1861 attgcacaag ataaaataat atcatcatga acaataaaac tgtctgctta cataaacagt
     1921 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     1981 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2041 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2101 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2161 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2221 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2281 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2341 aattgtcctt ttaacagcga tcgcgtattt cgcctcgctc aggcgcaatc acgaatgaat
     2401 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2461 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2521 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2581 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2641 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2701 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2761 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     2821 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3001 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3541 tt     
//
