LOCUS       pCFJ2443____1_2_        6216 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    677..715
                     /locus_tag="flex linker"
                     /label="flex linker"
                     /ApEinfo_label="flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  671..676
                     /locus_tag="NaeI"
                     /label="NaeI"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     570..665
                     /locus_tag="AttL1"
                     /label="AttL1"
                     /ApEinfo_label="AttL1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4295..4333
                     /locus_tag="Flex linker"
                     /label="Flex linker"
                     /ApEinfo_label="Flex linker"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4610..5416
                     /locus_tag="Kan (no BsmBI)"
                     /label="Kan (no BsmBI)"
                     /ApEinfo_label="Kan (no BsmBI)"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_structure  4334..4339
                     /locus_tag="NaeI(1)"
                     /label="NaeI(1)"
                     /ApEinfo_label="NaeI"
                     /ApEinfo_fwdcolor="#4c4c4c"
                     /ApEinfo_revcolor="#4c4c4c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     complement(4344..4439)
                     /locus_tag="AttL2"
                     /label="AttL2"
                     /ApEinfo_label="AttL2"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             join(716..724,769..918,1829..1946,2920..3101,4046..4294)
                     /locus_tag="mMaple3"
                     /label="mMaple3"
                     /ApEinfo_label="mMaple3"
                     /ApEinfo_fwdcolor="#00ff80"
                     /ApEinfo_revcolor="#00ff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(4477..4497)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(4457..4478)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5566..6154
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctCG GCCGGCAGTA CTAGCGGTGG CAGTGGAGGT ACCGGCGGAA GCAGTGTCTC
      721 CAAGgtaaca cttagttttt gttgctctgt ttaaaaatta attttaagGG AGAGGAGACT
      781 ATCATGTCCG TCATCAAGCC AGACATGAAG ATCAAGCTCC GTATGGAGGG AAACGTCAAC
      841 GGACACGCCT TCGTCATCGA GGGAGAGGGA TCCGGAAAGC CATTCGAGGG AATCCAAACC
      901 ATCGACCTCG AGGTCAAGgt aagttttctg ttttatttac ttgtttctga gaattgggat
      961 ttttagctga aaaattggat tttaagatga aaaattgaaa ttttcaacta aaaatttcaa
     1021 tttttagctg aaaagttaaa tttttagcta aagagtggga tttttagttg aaaaatcgag
     1081 atttctagct aaaaagtatg atttttagct gaaagattgg aattttaagc agaaaaaatt
     1141 gtatttttag ctgataaatc aaattttaag cggaaaaatt tgaattttta gctaaaaaat
     1201 tccattttca actaaaaatc gtgattttta gctttaaaaa ttggattttt agctgaaaaa
     1261 tcggattttt agctgaaaat tgaaattttt tagttaaaaa gtgagatttt ttaacagaaa
     1321 agtgagattt ttagttaaaa aataatattt ttagctaaaa aatacaattt tcaactaaag
     1381 atcgtgattt ttagctttaa aaattggatt tttagctaaa aaatcggatt tttagctgaa
     1441 aattgaaatt ttttagttaa aaagtgggat tttaagatga aaagtgagat tttttagctg
     1501 aaaagtgaga ttttcagcta aaaaatacaa ttttcaactg aaaaattggg atttttagct
     1561 ttaaaaattg gattttcagc tttaaaaatt ggatttttag ctgaaaagtg agatttttag
     1621 ttgaaaaatt gaaatgttta gctgaaaaat tggaattttt aagtaaaaaa gtgggatttt
     1681 aagatgaaaa gtgagatgtt tttagctgaa aagtgagatt tttagctaaa aaatacaatt
     1741 ttcaactgaa aaattgggat ttttagctca aaagtgggac gaaagacgaa aaaaaaaatc
     1801 gattttttac caaaaattag attttcagGA GGGAGCCCCA CTCCCATTCG CCTACGACAT
     1861 CCTCACCACC GCCTTCCACT ACGGAAACCG TGTCTTCACC AAGTACCCAC GAAAGATCCC
     1921 AGACTACTTC AAGCAATCCT TCCCAGgtaa gttatgtacg cgatttctcg gcgaaaaaat
     1981 ggactttttt gctcaaaaac tgaatttttc agcttttttt gcactgaaaa tgctgaaaat
     2041 tggttttttt gctgaaaatt caccgaaaaa agctgaaaat attgcagaaa tcgacgaaaa
     2101 atgccttttt tgctgaaatt tcagccagaa aattgttcag aatcgcaatt tcctgccaaa
     2161 atcgacgttt ttcggcgaaa attggtagat tttgaaccga aaattgtgaa aaatggactt
     2221 ttttgctcaa aaacagactt tttgagcttt tttcacactg aaaatggtgg aaaattggat
     2281 tttccagcag aaaattcgga aattttcaat ttttggcgga aatttgacga aaaatatatg
     2341 aaaaacaccg atttttgtct gaaaaagttc aaaagccgaa aaattggcca aaaatctcca
     2401 aaatcggact tttctggcgt ttttcgtgga aaaattgaaa tttttttgaa aaattgagca
     2461 aaaatcgccg aaaaaccgct aaaaaaactg ccgaaattgg caattttgag cttaaaaaaa
     2521 cttgaaaaat gctcaaaatt acccaatttt caccggaaat tgagctaaaa tcgtcgaaaa
     2581 agactgaatt ttcaattttt agctgaaaat ctgcctattt tcggctaaaa ttgctttaaa
     2641 aacattaaaa ttcaaatttt ttggctaaaa atactcagat tgcccttttt tttctccaaa
     2701 aatgacaaaa aaaaaatcaa tcagaaaaat gcgatttttt ttacaaaaat tggcttcaaa
     2761 acgtctaaat tctccctaat ttttgctgct gaaaattgtg caaaaatcac cgaaaaatga
     2821 cgattttagg ctgaaaatct tcaaaaaaag ctcaaaattc cccacttttc accggaaaaa
     2881 gacgaaaaaa gctccaaatt tatcgattaa cattttcagA GGGATACTCC TGGGAGCGTT
     2941 CCATGACCTA CGAGGACGGA GGAATCTGCA ACGCCACCAA CGACATCACC ATGGAGGAGG
     3001 ACTCCTTCAT CAACAAGATC CACTTCAAGG GAACCAACTT CCCACCAAAC GGACCAGTCA
     3061 TGCAAAAGCG TACCGTCGGA TGGGAGGTTT CCACCGAGAA Ggtaagttct tttttttgct
     3121 cgtttttgag tgaaaaattg atggaattca caaaaaatgg ccaaagaatc ctcaaaattg
     3181 gccgaaaatt ctgaaaaatg acctaaaaat caccgaaaaa cggccaaaaa ttatctgaaa
     3241 atcgccgaaa atctcaaatt ttgaggaaat tcccgctaaa aattaataaa aatcaccgaa
     3301 aaaaggccaa aaattaccta ataaaggccg aaaatcgcct aaaaaggcca aaaattctga
     3361 aaaatggcca aatttcaatt tttcacacac aaaaaatccc tgaattttct atttttactc
     3421 gaaaaactgt ggatttacgg gggtaaaaat tcagttttta gtgctatttc acaagaaaaa
     3481 cacagcattt tcgacaaaaa ctccgatttt taagccaaaa atttgaggtt tttagttttt
     3541 tacgtgaaaa cctgaacttt ttgccaaaaa aacacttttt tttagactaa aaatcaattt
     3601 tcgatcaaaa atcactaatt ttgcctgaaa aaatatagat ttttccactt tttcactgaa
     3661 aaaaaactgg attttcacta gaaaatcaag ttgtttttag accaaaaatt ttatttttcc
     3721 gctgaaaatt atcgtttttc agtcaatttt cagctaaaaa tccctgaaat tctggatttt
     3781 cagccaaaaa actcgaaatt ttccaaaaaa accccgttta aaaatgagtt tagactgaaa
     3841 attccgattt ttgagccaaa aatttgattt ttaatcgaaa aaaattggat tttcaaccaa
     3901 aaaatcagtt tttcagtgat ttttagagta aaaatcactg attttaacac aaaattccag
     3961 tttttcgtga tttttcacta gaaaatcccg atttttcccc aaaaaatcca tttttccgac
     4021 taaaaatccc caaagtaatt ttcagATGTA CGTCCGTGAC GGAGTCCTCA AGGGTGACGT
     4081 CAAGATGAAG CTCCTCCTCA AGGGAGGATC CCACTACCGT TGCGACTTCC GTACCACCTA
     4141 CAAGGTCAAG CAAAAGGCCG TCAAGCTCCC AAAGGCCCAC TTCGTCGACC ACCGTATCGA
     4201 GATCCTCTCC CACGACAAGG ACTACAACAA GGTCAAGCTC TACGAGCACG CCGTCGCCCG
     4261 TAACTCCACC GACTCCATGG ACGAGCTCTA CAAGGGTGGC ACAGGAGGAA CGGGCGGTAG
     4321 TGGAGGCACT GGTGCCGGCA acccagcttt cttgtacaaa gttggcatta taagaaagca
     4381 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     4441 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     4501 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa taatatcatc
     4561 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     4621 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     4681 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     4741 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     4801 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     4861 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     4921 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     4981 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     5041 atttcgcctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     5101 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     5161 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     5221 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     5281 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     5341 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     5401 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     5461 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     5521 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5581 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5641 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5701 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5761 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5821 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5881 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5941 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6001 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6061 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6121 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6181 gttcctggcc ttttgctggc cttttgctca catgtt  
//
