LOCUS       pCFJ2370___intro        2830 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     272 bases. 261 bases in phase. PATC density = 423
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1224..2030
                     /locus_tag="Kan (no BsmBI)"
                     /label="Kan (no BsmBI)"
                     /ApEinfo_label="Kan (no BsmBI)"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     669..679
                     /locus_tag="Golden Gate 3A"
                     /label="Golden Gate 3A"
                     /ApEinfo_label="Golden Gate 3A"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     944..954
                     /locus_tag="Golden Gate 3B"
                     /label="Golden Gate 3B"
                     /ApEinfo_label="Golden Gate 3B"
                     /ApEinfo_fwdcolor="#57bffe"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          676..947
                     /locus_tag="250 bp intron (272 bases. 261 bases in phase.
                     PATC density = 423)"
                     /label="250 bp intron (272 bases. 261 bases in phase. PATC
                     density = 423)"
                     /ApEinfo_label="250 bp intron (272 bases. 261 bases in
                     phase. PATC density = 423)"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     537..553
                     /locus_tag="M13F"
                     /label="M13F"
                     /ApEinfo_label="M13F"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1095..1111)
                     /locus_tag="M13R"
                     /label="M13R"
                     /ApEinfo_label="M13R"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2180..2768)
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgg tctcacttta cggagaaaat gcggaaaatt gtgaaaaaaa aacacttttt
      721 tgaatgaaaa atcgaaattt ttcgtatttt ttactgaaaa aatcggaatt tttcgtatta
      781 tttagtgaaa aaatcgaaaa atgttataat ttttgacgaa atttatagca aaaaatcaca
      841 atttttgcca tttctagtcg ttttcattgt aaaaaccaga aaattgtatg attttgacag
      901 aaaaattcaa gaatttgagc gttttttaac caaaaaaaaa ctggtaaaga gaccacccag
      961 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1021 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     1081 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1141 gatgttacat tgcacaagat aaaataatat catcatgaac aataaaactg tctgcttaca
     1201 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     1261 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     1321 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     1381 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     1441 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     1501 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     1561 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     1621 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg cctcgctcag gcgcaatcac
     1681 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     1741 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     1801 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     1861 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     1921 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     1981 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     2041 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     2101 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2161 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2221 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2281 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2341 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2401 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2461 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2521 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2581 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2641 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2701 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2761 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2821 ctcacatgtt     
//
