LOCUS       pCFJ2366___intro        2816 bp    DNA        circular     14-AUG-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     258 bases. 63 bases in phase. PATC density = 36
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1210..2016
                     /locus_tag="Kan (no BsmBI)"
                     /label="Kan (no BsmBI)"
                     /ApEinfo_label="Kan (no BsmBI)"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="#ffff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1077..1097)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1057..1078)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     669..679
                     /locus_tag="Golden Gate 3A"
                     /label="Golden Gate 3A"
                     /ApEinfo_label="Golden Gate 3A"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#66ccff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     930..940
                     /locus_tag="Golden Gate 3B"
                     /label="Golden Gate 3B"
                     /ApEinfo_label="Golden Gate 3B"
                     /ApEinfo_fwdcolor="#57bffe"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          676..933
                     /locus_tag="XK370.5 intron (258 bases. 63 bases in phase.
                     PATC density = 36)"
                     /label="XK370.5 intron (258 bases. 63 bases in phase. PATC
                     density = 36)"
                     /ApEinfo_label="XK370.5 intron (258 bases. 63 bases in
                     phase. PATC density = 36)"
                     /ApEinfo_fwdcolor="#66ffcb"
                     /ApEinfo_revcolor="#66ffcb"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     537..553
                     /locus_tag="M13F"
                     /label="M13F"
                     /ApEinfo_label="M13F"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1081..1097)
                     /locus_tag="M13R"
                     /label="M13R"
                     /ApEinfo_label="M13R"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2166..2754)
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgg tctcacttta tgaagatacg aattaaaaaa aagacgacgc ctcttcatca
      721 cttcaatgta acaaagtgcc tttttgaacg cacacacttc ttccccacgt gctgttgcag
      781 ttttgatact aaaaaaacga tagaaaaaga gagataagag atgaaaatga taagagaatt
      841 ggaaaaagag gaattaaaaa aggaaacgat taagttgaaa gtagaaagaa cagaaaaatg
      901 tgtatggagg ggttttcaaa ttctcttttg taaagagacc acccagcttt cttgtacaaa
      961 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1021 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1081 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1141 caagataaaa taatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1201 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1261 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1321 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1381 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     1441 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1501 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1561 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     1621 ccttttaaca gcgatcgcgt atttcgcctc gctcaggcgc aatcacgaat gaataacggt
     1681 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1741 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1801 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     1861 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     1921 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     1981 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2041 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2101 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2161 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2221 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2281 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2341 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2401 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2461 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2521 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2581 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2641 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2701 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2761 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
