LOCUS       pCFJ1117                3636 bp    DNA        circular     14-DEC-2020
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
COMMENT     Aljohani et al. 2020
COMMENT     [4-1] Gateway compatible promoter with no ATG in promoter (so,
            include a start site in the [1-2] slot).
COMMENT     Psmu-1 drives robust germline expression and some somatic
            expression from extrachromosomal arrays.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     536..553
                     /locus_tag="M13-fwd"
                     /label="M13-fwd"
                     /ApEinfo_label="M13-fwd"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1897..1917)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2030..2836
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        689..1680
                     /locus_tag="Psmu-1"
                     /label="Psmu-1"
                     /ApEinfo_label="Psmu-1"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="#ff8000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1877..1898)
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1689..1838
                     /locus_tag="attP1R"
                     /label="attP1R"
                     /ApEinfo_label="attP1R"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    593..674
                     /locus_tag="attP4"
                     /label="attP4"
                     /ApEinfo_label="attP4"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="#ff80ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     537..553
                     /locus_tag="M13F"
                     /label="M13F"
                     /ApEinfo_label="M13F"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1901..1917)
                     /locus_tag="M13R"
                     /label="M13R"
                     /ApEinfo_label="M13R"
                     /ApEinfo_fwdcolor="#72fa78"
                     /ApEinfo_revcolor="#ff7d78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2986..3574
                     /locus_tag="Ori"
                     /label="Ori"
                     /ApEinfo_label="Ori"
                     /ApEinfo_fwdcolor="#d5d5d5"
                     /ApEinfo_revcolor="#d5d5d5"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgaa atttttcagg cgggaagtgg cttcggtgcg
      721 ttcaacaaac gcgcttttga ctcgctagcc ggatccggct tcagcggttt tgacaaaaga
      781 gctttcgatt cactggctgg acaaggtttt actggatttg agaaacgcgc ctttgataca
      841 gtggtacggt tttcggtgga aattccagtt tttcgaattt tcaataattt tcagagcacg
      901 tcaggctttg acgatttcaa attgtgaaga gctcactgat atttaaaatt tggttcaatt
      961 caagcacata tttttgaggt aagcaactga ttttaatgtt gttaatcaaa tgaaaagtga
     1021 aatttttgga ataatttttt tttgttttca ggtagctcca acgaacgaca cacacaagcc
     1081 cagtccccca gtattcctga cgacagcccc tgctcacaca ctttttatta taattccttt
     1141 tttttcaaac ttttttttca aattttatac tttaatctca aaaaacctat tcatacgaac
     1201 acaagtaccg gtgtgcgtgt gtgagaataa gaatttattt taaaaaaata aagaatgtcg
     1261 tcctgttttt tcatatttat tgaagaaaat ggagctggaa aaaaatcaat tgtaaaaaac
     1321 agaaaaacgt gctgataaaa tgcattgtac gcaaactgtg gattttgaga actaacgtag
     1381 tcaagcaaaa atctctggaa ttttatcgat ttttaacgga taaactgttg ttttttgtaa
     1441 ctgtgcatga aaaaaaatct caggcaaaaa tattttctga aaaaaaaaga gtttaaacac
     1501 aaatgcaagt ccacaaaaaa ctccaatttg aaattcgctc caaaaacgca aaacacgcca
     1561 agcgctcgtg ctggcgcatt tgccgatcgc ttccaaaaag gctggcgcac ttctcgtgtc
     1621 cgaggatatt cgaattttac acgtcatttt cagccgtttt tcaccgaatt ttcgctgaaa
     1681 acaagtttgt acaaaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta
     1741 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatgcagtca
     1801 ctatgaacca actacttaga tggtattagt gacctgtaga attcgagctc tagagctgca
     1861 gggcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     1921 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     1981 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     2041 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     2101 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     2161 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     2221 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     2281 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     2341 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     2401 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     2461 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     2521 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     2581 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     2641 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     2701 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     2761 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     2821 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     2881 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     2941 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3001 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3121 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3361 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3601 gttcctggcc ttttgctggc cttttgctca catgtt  
//
