LOCUS pSicoR_GFP_RPL10 8295 bp ds-DNA circular 23-JAN-2019 DEFINITION synthetic circular DNA. ACCESSION VERSION . KEYWORDS pSicoR-Ef1a-mCh-Puro. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct. REFERENCE 1 (bases 1 to 8141) AUTHORS Salomonis N, Schlieve CR, Pereira L, Wahlquist C, Colas A, Zambon AC, Vranizan K, Spindler MJ, Pico AR, Cline MS, Clark TA, Williams A, Blume JE, Samal E, Mercola M, Merrill BJ and Conklin BR TITLE Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. JOURNAL Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10514-9. Epub 2010 May 24. PUBMED 20498046 REFERENCE 2 (bases 1 to 8141) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com COMMENT COMMENT COMMENT ApEinfo:methylated:1. FEATURES Location/Qualifiers primer_bind complement(56..75) /note="pRS vectors, use to sequence yeast selectable marker" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="pRS-marker" enhancer 247..626 /note="human cytomegalovirus immediate early enhancer" /ApEinfo_fwdcolor="#5ac3fa" /ApEinfo_revcolor="#5ac3fa" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="CMV enhancer" promoter 628..826 /note="human cytomegalovirus (CMV) immediate early promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="CMV promoter" primer_bind 781..801 /note="Human CMV immediate early promoter, forward primer" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="CMV-F" LTR 844..1024 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /ApEinfo_fwdcolor="#86f71d" /ApEinfo_revcolor="#d7336f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="5' LTR (truncated)" misc_feature 1071..1196 /note="packaging signal of human immunodeficiency virus type 1" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="HIV-1 Psi" misc_feature 1695..1928 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="RRE" misc_feature 2455..2572 /note="central polypurine tract and central termination sequence of HIV-1" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="cPPT/CTS" protein_bind 2825..2858 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="loxP" promoter 3018..3229 /note="core promoter for human elongation factor EF-1-alpha" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="EF-1-alpha core promoter" LTR 3242..3510 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /ApEinfo_label="5' LTR (truncated)" /ApEinfo_fwdcolor="#86f71d" /ApEinfo_revcolor="#d7336f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="5' LTR (truncated)(1)" regulatory 3548..3557 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)" /label="vertebrate consensus sequence for strong initiation" CDS 3554..3574 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ YERA EGRHSTGGMDELYK" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="mCherry" misc_feature 3575..4960 /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="GFP-RPL10a" misc_feature 3575..4309 /ApEinfo_fwdcolor="#07ff15" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="GFP" misc_feature 4269..5008 /ApEinfo_fwdcolor="#fff30a" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="confirmed by sequencing (WPRE-REV)" protein_bind 5095..5128 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." /ApEinfo_label="loxP" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="loxP(1)" misc_feature 5184..5772 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="WPRE" primer_bind complement(5237..5257) /note="WPRE, reverse primer" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="WPRE-R" CDS complement(5655..5666) /codon_start=1 /product="Factor Xa recognition and cleavage site" /translation="IEGR" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="Factor Xa site" primer_bind complement(5775..5791) /note="common sequencing primer, one of multiple similar variants" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="KS primer" primer_bind complement(5776..5792) /note="For pBluescript vector" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="pBluescriptKS" LTR 6301..6481 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /ApEinfo_label="5' LTR (truncated)" /ApEinfo_fwdcolor="#86f71d" /ApEinfo_revcolor="#d7336f" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="5' LTR (truncated)(2)" rep_origin complement(6543..7131) /direction="LEFT" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="ori" primer_bind complement(6623..6642) /note="pBR322 origin, forward primer" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="pBR322ori-F" CDS complement(7302..8162) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGAS LIKHW" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="AmpR" primer_bind 7925..7944 /note="Ampicillin resistance gene, reverse primer" /ApEinfo_fwdcolor="#14c0bd" /ApEinfo_revcolor="#4ec02b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="Amp-R" promoter complement(8163..8267) /gene="bla" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /label="AmpR promoter" ORIGIN 1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa 61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct 1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa 1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa 1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg 1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag 1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc 1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa 1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg 1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag 2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa 2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg 2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga 2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag 2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc 2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac 2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa 2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc 2641 gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc tcggctactc 2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga 2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat 2821 ttctataact tcgtatagca tacattatac gaagttatac atgtcacaaa aggaaactca 2881 ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga gaaaagcctt 2941 gttaacgcgc ggtgaccctc gagtactagg atccattagg cggccgcgga tctgcgatcg 3001 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 3061 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 3121 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 3181 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 3241 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 3301 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 3361 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 3421 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 3481 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagcgct 3541 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 3601 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 3661 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 3721 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 3781 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 3841 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 3901 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 3961 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 4021 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 4081 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 4141 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 4201 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 4261 gctgtacaag tccggccgga ctcagatctc gagctcaagc ttcgaattca gcagcaaagt 4321 ctcacgcgac accctgtacg aggcggtgcg ggaagtcctg cacgggaacc agcgcaagcg 4381 ccgcaagttt ctggagacgg tggagctgca gatcagcctg aagaactacg accctcagaa 4441 ggacaaacgt ttctcgggca ccgtcaggct caagtccacc ccacgcccca agttctcggt 4501 gtgcgttctg ggggaccagc agcactgtga tgaagccaag gccgtggata tcccccacat 4561 ggacatcgag gcgctcaaga agcttaacaa aaacaagaag ttggtcaaga agctggctaa 4621 gaagtacgat gcctttttgg cctctgagtc tctgattaag cagatcccac gtatcctggg 4681 cccaggccta aacaaggctg gcaagttccc ctccctgctg acacacaatg aaaacatggt 4741 ggccaaagtg gatgaggtga aatcgacaat caagttccag atgaagaagg tgctgtgttt 4801 ggccgtcgct gttggccacg tgaagatgac cgatgatgag ctagtctaca acattcatct 4861 ggctgtcaat ttcttggtgt ccttgcttaa gaaaaactgg caaaacgtgc gggctctgta 4921 catcaagagc accatgggca agccccagcg tctgtattag ggatccaccg gatctagata 4981 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 5041 acacctcccc ctgaacctga aacataaaat gaatgcaatt cgtcgaggga cctaataact 5101 tcgtatagca tacattatac gaagttatac atgtttaagg gttccggttc cactaggtac 5161 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 5221 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 5281 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 5341 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 5401 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 5461 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 5521 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 5581 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 5641 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 5701 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 5761 ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc 5821 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag 5881 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca 5941 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 6001 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat 6061 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac 6121 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc 6181 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg 6241 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact 6301 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 6361 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6421 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6481 agcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 6541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 6601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 6661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 6721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 6781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 6841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 6901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 6961 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 7021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 7081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 7141 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 7201 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 7261 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 7321 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 7381 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 7441 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 7501 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 7561 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 7621 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 7681 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 7741 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 7801 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 7861 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 7921 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 7981 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 8041 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 8101 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 8161 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 8221 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 8281 aaagtgccac ctgac //