LOCUS       SRC-116v5_-_huAD        7049 bp    DNA     circular UNA 30-JUL-2018
DEFINITION  Gibson Assembly ligation product.
ACCESSION   urn.local...t-9bmygl8
VERSION     urn.local...t-9bmygl8
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      complement(182..770)
                     /label="Rep_Origin_1"
     CDS             complement(941..1801)
                     /label="bla"
     primer_bind     1069..1088
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.7"
                     /Pair_Dimer_Tm="3.4"
                     /Sequence="CAATGATACCGCGACTCCCA"
                     /Product_Size=42
                     /annotation_group="422581157225000d2beb8d-972f-44d9-9835-9
                     b9e8bbd45adurn:sequence:genbank:null"
                     /label="1,069 F"
     misc_feature    complement(1069..1089)
                     /created_by="primer3"
                     /%GC="57.1"
                     /Tm="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.1"
                     /Sequence="GTGGGAGTCGCGGTATCATTG"
                     /annotation_group="42293062508962887945b9-cd2b-4ccb-a09a-9
                     accad3006c9urn:sequence:genbank:null"
                     /label="1,089 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    1083..1084
                     /Original_Bases="GA"
                     /label="GA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(1482..1504)
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="60.2"
                     /Hairpin_Tm="39.8"
                     /Self_Dimer_Tm="29.8"
                     /Sequence="TTGGTTGAGTACTCACCAGTCAC"
                     /annotation_group="38990488321597f7566cb3-8e5b-44c2-aa7e-3
                     aed6a6214b2urn:sequence:genbank:null"
                     /label="1,504 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1483..1505)
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="59.9"
                     /Hairpin_Tm="39.8"
                     /Self_Dimer_Tm="24.5"
                     /Sequence="CTTGGTTGAGTACTCACCAGTCA"
                     /annotation_group="3899048673436687edf077-fdf0-440c-8bd3-5
                     9767432625curn:sequence:genbank:null"
                     /label="1,505 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1484..1506)
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="59.9"
                     /Hairpin_Tm="39.8"
                     /Self_Dimer_Tm="27.1"
                     /Sequence="ACTTGGTTGAGTACTCACCAGTC"
                     /annotation_group="3899048780697159646bf0-340f-4cdf-bc4b-b
                     d9f044f481furn:sequence:genbank:null"
                     /label="1,506 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1485..1507)
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="59.9"
                     /Hairpin_Tm="39.8"
                     /Self_Dimer_Tm="27.1"
                     /Sequence="GACTTGGTTGAGTACTCACCAGT"
                     /annotation_group="38990487286253cef0506b-1e99-4651-8287-3
                     eddaca00f59urn:sequence:genbank:null"
                     /label="1,507 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1486..1508)
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="59.9"
                     /Hairpin_Tm="39.8"
                     /Self_Dimer_Tm="26.1"
                     /Sequence="TGACTTGGTTGAGTACTCACCAG"
                     /annotation_group="38990486017975205cc7af-ed84-4d15-93bf-0
                     6f6b8a8dbc2urn:sequence:genbank:null"
                     /label="1,508 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1512..1532)
                     /created_by="primer3"
                     /%GC="47.6"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCGCCGCATACACTATTCTCA"
                     /annotation_group="223495202346043c95bfe8e-844d-4d48-8b6b-
                     fdf578ea5337urn:sequence:genbank:null"
                     /label="1,532 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1514..1534)
                     /created_by="primer3"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGTCGCCGCATACACTATTCT"
                     /annotation_group="2234952012760895a9008dc-be86-42db-bb84-
                     25b05331d055urn:sequence:genbank:null"
                     /label="1,534 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1515..1534)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="58.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGTCGCCGCATACACTATTC"
                     /annotation_group="223495200213591dc0f01bd-f1ba-482b-bea5-
                     3f6122f9e301urn:sequence:genbank:null"
                     /label="1,534 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1516..1535)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.6"
                     /Hairpin_Tm="41.2"
                     /Self_Dimer_Tm="5.4"
                     /Sequence="CGGTCGCCGCATACACTATT"
                     /annotation_group="22349519512392570224544-3298-489e-ac33-
                     c4cb5f1dd0b8urn:sequence:genbank:null"
                     /label="1,535 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1517..1535)
                     /created_by="primer3"
                     /%GC="57.9"
                     /Tm="60.0"
                     /Hairpin_Tm="41.2"
                     /Self_Dimer_Tm="5.4"
                     /Sequence="CGGTCGCCGCATACACTAT"
                     /annotation_group="2234951987954159f7cb21b-206a-4cba-8bec-
                     9c8350db5f27urn:sequence:genbank:null"
                     /label="1,535 R"
                     /note="Geneious type: primer_bind_reverse"
     sig_peptide     complement(1733..1801)
                     /label="bla"
     misc_feature    complement(1843..1871)
                     /label="amp prom"
                     /note="Geneious type: promoter prokaryotic"
     primer_bind     2397..2415
                     /created_by="primer3"
                     /%GC="57.9"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="27.0"
                     /Sequence="CATTCAGGCTGCGCAACTG"
                     /annotation_group="67824515884868b6f67138-be36-40ee-8aff-0
                     8c7bab054d3urn:sequence:genbank:null"
                     /label="2,397 F"
     primer_bind     2398..2417
                     /created_by="primer3"
                     /%GC="50.0"
                     /Tm="61.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="29.6"
                     /Sequence="ATTCAGGCTGCGCAACTGTT"
                     /annotation_group="6782451724125316746918-2f4c-4fe1-9306-2
                     b1901d3c84furn:sequence:genbank:null"
                     /label="2,398 F"
     primer_bind     2410..2430
                     /created_by="primer3"
                     /%GC="57.1"
                     /Tm="61.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.7"
                     /Sequence="CAACTGTTGGGAAGGGCGATC"
                     /annotation_group="67824514103496e5690b8d-2bff-4e43-913c-2
                     34b25a8fd95urn:sequence:genbank:null"
                     /label="2,410 F"
     primer_bind     2410..2429
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.7"
                     /Sequence="CAACTGTTGGGAAGGGCGAT"
                     /annotation_group="6782451500316960c9ada5-79b0-473f-b282-9
                     f7367e27557urn:sequence:genbank:null"
                     /label="2,410 F"
     primer_bind     2411..2430
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AACTGTTGGGAAGGGCGATC"
                     /annotation_group="67824512751645ea8bb395-6e8f-43a4-8536-0
                     d421358a3f3urn:sequence:genbank:null"
                     /label="2,411 F"
     repeat_region   2458..2647
                     /label="ITR"
     misc_feature    complement(2464..2482)
                     /created_by="primer3"
                     /%GC="68.4"
                     /Tm="63.3"
                     /Hairpin_Tm="43.0"
                     /Self_Dimer_Tm="7.3"
                     /Sequence="CTCAGTGAGCGAGCGAGCG"
                     /annotation_group="38321821880942cba41728-ddde-47a0-8a5b-c
                     09cb4dfe246urn:sequence:genbank:null"
                     /label="2,482 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2465..2483)
                     /created_by="primer3"
                     /%GC="68.4"
                     /Tm="63.0"
                     /Hairpin_Tm="37.4"
                     /Self_Dimer_Tm="8.6"
                     /Sequence="CCTCAGTGAGCGAGCGAGC"
                     /annotation_group="38321821118590d0917246-e3ed-4f4e-a106-5
                     cd6f24dd65furn:sequence:genbank:null"
                     /label="2,483 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2466..2484)
                     /created_by="primer3"
                     /%GC="68.4"
                     /Tm="63.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="13.0"
                     /Sequence="GCCTCAGTGAGCGAGCGAG"
                     /annotation_group="38321819946915d0d8c7a3-867d-406f-9427-9
                     f547b242071urn:sequence:genbank:null"
                     /label="2,484 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2467..2485)
                     /created_by="primer3"
                     /%GC="68.4"
                     /Tm="64.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="13.3"
                     /Sequence="GGCCTCAGTGAGCGAGCGA"
                     /annotation_group="383218226198282e23d756-2fc5-4ef0-b286-0
                     9f39975cab3urn:sequence:genbank:null"
                     /label="2,485 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2499..>3840
                     /created_by="User"
                     /label="XmaEcoEf1"
     primer_bind     2598..2620
                     /created_by="primer3"
                     /%GC="43.5"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGATTAACCCGCCATGCTACTTA"
                     /annotation_group="1933337599281608ca541fb-3daf-48dd-947e-
                     c584a33b1960urn:sequence:genbank:null"
                     /label="2,598 F"
     primer_bind     2603..2625
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AACCCGCCATGCTACTTATCTAC"
                     /annotation_group="193333765455348f35318fe-ffc6-4ea7-98fb-
                     c260ffef3a63urn:sequence:genbank:null"
                     /label="2,603 F"
     primer_bind     2606..2628
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCGCCATGCTACTTATCTACGTA"
                     /annotation_group="193333764397582cc881276-fc90-4d2a-800a-
                     4e5d35a38189urn:sequence:genbank:null"
                     /label="2,606 F"
     primer_bind     2622..2644
                     /created_by="primer3"
                     /%GC="52.2"
                     /Tm="60.1"
                     /Hairpin_Tm="40.4"
                     /Self_Dimer_Tm="10.1"
                     /Sequence="CTACGTAGCCATGCTCTAGGAAG"
                     /annotation_group="193333759001094f9c13a6f-0a05-4800-82a8-
                     79b0823f6ef1urn:sequence:genbank:null"
                     /label="2,622 F"
     primer_bind     2630..2652
                     /created_by="primer3"
                     /%GC="52.2"
                     /Tm="59.8"
                     /Hairpin_Tm="37.4"
                     /Self_Dimer_Tm="1.3"
                     /Sequence="CCATGCTCTAGGAAGATCGTAGG"
                     /annotation_group="1933337649909832f825e58-2855-41fd-a15d-
                     54f28529febdurn:sequence:genbank:null"
                     /label="2,630 F"
     misc_difference 2661..3839
                     /label="Human EF1a Promoter"
     source          join(<5833..>5851,<3841..>4634)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     3841..3857
                     /created_by="primer3"
                     /%GC="64.7"
                     /Tm="59.7"
                     /Hairpin_Tm="41.3"
                     /Self_Dimer_Tm="23.5"
                     /Pair_Dimer_Tm="1.0"
                     /Sequence="ATCCATCGATCCGCGGC"
                     /Product_Size=3263
                     /annotation_group="51615962970404150ec2f9-47e8-492a-89a7-0
                     424dde992bcMCPEgTbr - GS59656-7
                     pTRE3G-BI-NLS-mCherry-6XMS2-MCPEgTbr PCR Product 2"
                     /Extension="CATTTCAGGTGTCGTGAG"
                     /label="MCPEgTbr - GS59656-7
                     pTRE3G-BI-NLS-mCherry-6XMS2-MCPEgTbr PCR Product 2 (fwd) -
                     Digestion of pAAV-EF1a-FLEX-r - Fragment 2"
     primer_bind     3841..3856
                     /Sequence="ATCCATCGATCCGCGG"
                     /Hairpin_Tm="41.3"
                     /Tm="55.9"
                     /Self_Dimer_Tm="22.1"
                     /%GC="62.5"
                     /modified_by="User"
                     /label="SGR-82B"
     primer_bind     complement(3856..3870)
                     /Sequence="ACCCATGGTGGCGGC"
                     /Hairpin_Tm="33.0"
                     /Tm="59.2"
                     /Self_Dimer_Tm="8.1"
                     /%GC="73.3"
                     /modified_by="User"
                     /label="SGR-90"
     CDS             3865..3869
                     /codon_start=1
                     /note="MetGly"
                     /translation="M"
                     /label="MetGly CDS"
     CDS             3871..3891
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
                     /label="nuclear localization signal of SV40 large T 
                     antigen CDS"
     primer_bind     complement(3883..3900)
                     /Sequence="CTTCACACCCACTTTGCG"
                     /Hairpin_Tm="None"
                     /Tm="56.7"
                     /Self_Dimer_Tm="None"
                     /%GC="55.6"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="SGR-45"
     CDS             3892..4599
                     /codon_start=1
                     /note="MCP Double"
                     /translation="GVKMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQ
                     AYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGL
                     LKDGNPIPSAIAANSGIYAMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSR
                     SQAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQ
                     GLLKDGNPIPSAIAANSG"
                     /label="MCP Double CDS"
     primer_bind     3892..3906
                     /Sequence="GGTGTGAAGATGGCC"
                     /Hairpin_Tm="None"
                     /Tm="50.4"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /label="SGR-91"
     CDS             4600..>4634
                     /codon_start=1
                     /note=12
                     /translation="GGSGGTGGSGGT"
                     /label="12 CDS"
     ligation        4613..4634
                     /label="Ligation"
     CDS             4636..>5160
                     /created_by="Sam"
                     /label="huADAR2"
     misc_feature    4636..4660
                     /Sequence="CAGCTGCATTTACCGCAGGTTTTAG"
                     /Hairpin_Tm="80.5"
                     /Tm="63.1"
                     /Self_Dimer_Tm="13.6"
                     /%GC="48.0"
                     /Extension="GAACTGGTGGCTCTGGTGGTACC"
                     /modified_by="Sam"
                     /label="SGR-207"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5152..5160
                     /Original_Bases="GAAGGGGCT"
                     /label="GAAGGGGCT"
                     /note="Geneious type: Editing History Replacement"
     CDS             <5161..5790
                     /codon_start=1
                     /created_by="Sam"
                     /label="huADAR2"
     misc_feature    complement(5804..5828)
                     /Sequence="GGGTTTAAACGGGCCCTCTAGACTC"
                     /Hairpin_Tm="38.1"
                     /Tm="64.6"
                     /Self_Dimer_Tm="22.4"
                     /%GC="56.0"
                     /created_by="primer3"
                     /Extension="ATGATCCTCTGGAGATATCACTAG"
                     /label="SGR-208"
                     /note="Geneious type: primer_bind_reverse"
     ligation        5833..5852
                     /label="Ligation"
     enhancer        5858..6447
                     /label="WPRE"
     primer_bind     5858..5884
                     /Sequence="TAATCAACCTCTGGATTACAAAATTTG"
                     /Hairpin_Tm="38.0"
                     /Tm="56.9"
                     /Self_Dimer_Tm="3.9"
                     /%GC="29.6"
                     /created_by="primer3"
                     /label="SGR-81"
     polyA_signal    6488..6701
                     /label="bGH-PolyA"
     primer_bind     6662..6684
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="60.1"
                     /Hairpin_Tm="40.9"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGGATTGGGAAGACAATAGCAGG"
                     /annotation_group="388611594275331d490953-e17e-4c10-8c76-f
                     f24ef299a2durn:sequence:genbank:null"
                     /label="6,701 F"
     primer_bind     6664..6686
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="60.9"
                     /Hairpin_Tm="40.9"
                     /Self_Dimer_Tm="None"
                     /Sequence="GATTGGGAAGACAATAGCAGGCA"
                     /annotation_group="38861160544800ef2a8452-bc47-4478-8679-4
                     0f500624523urn:sequence:genbank:null"
                     /label="6,703 F"
     primer_bind     6665..6687
                     /created_by="primer3"
                     /%GC="43.5"
                     /Tm="60.4"
                     /Hairpin_Tm="39.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTGGGAAGACAATAGCAGGCAT"
                     /annotation_group="38861159888543caa2b782-20a6-4e7d-9cc0-f
                     55cadb5b429urn:sequence:genbank:null"
                     /label="6,704 F"
     primer_bind     6665..6686
                     /created_by="primer3"
                     /%GC="45.5"
                     /Tm="60.0"
                     /Hairpin_Tm="39.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTGGGAAGACAATAGCAGGCA"
                     /annotation_group="388611614573646ed842e8-8321-42db-9dea-1
                     b2c092774e9urn:sequence:genbank:null"
                     /label="6,704 F"
     primer_bind     6666..6687
                     /created_by="primer3"
                     /%GC="45.5"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTGGGAAGACAATAGCAGGCAT"
                     /annotation_group="38861161018074c87b0543-e37e-4586-be8b-2
                     e53fb894827urn:sequence:genbank:null"
                     /label="6,705 F"
     misc_feature    complement(6724..6747)
                     /created_by="primer3"
                     /%GC="41.7"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCCCCCTGAACCTGAAACATAAAA"
                     /annotation_group="1008173670300384e9d097a-a38a-44aa-8cb5-
                     29d3dcdb060burn:sequence:genbank:null"
                     /label="6,786 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6724..6746)
                     /created_by="primer3"
                     /%GC="43.5"
                     /Tm="58.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCCCCTGAACCTGAAACATAAAA"
                     /annotation_group="10081736815574130300dd9-23e0-4413-a0c9-
                     4ebb4d7469ddurn:sequence:genbank:null"
                     /label="6,785 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6725..6747)
                     /created_by="primer3"
                     /%GC="43.5"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCCCCCTGAACCTGAAACATAAA"
                     /annotation_group="10081736456771065866f53-3ce0-4ec3-9a4c-
                     b8eaa1cbe9c8urn:sequence:genbank:null"
                     /label="6,786 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6726..6748)
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTCCCCCTGAACCTGAAACATAA"
                     /annotation_group="100817348844182d010e2b0-c411-4d5e-a75c-
                     cdd33be12cf2urn:sequence:genbank:null"
                     /label="6,787 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6727..6749)
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCTCCCCCTGAACCTGAAACATA"
                     /annotation_group="100817365900416e544093f-c5bf-451d-b9c8-
                     6fc903ce9538urn:sequence:genbank:null"
                     /label="6,788 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6729..6748)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="59.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTCCCCCTGAACCTGAAACA"
                     /annotation_group="1019056916699158c30084d-0229-43af-b1ad-
                     f0d92575d3dfurn:sequence:genbank:null"
                     /label="6,787 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6733..6752)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACATCTCCCCCTGAACCTGA"
                     /annotation_group="101905678106941439e3fdf-5ba7-4534-ad27-
                     a36aff83165curn:sequence:genbank:null"
                     /label="6,791 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6734..6753)
                     /created_by="primer3"
                     /%GC="60.0"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CACATCTCCCCCTGAACCTG"
                     /annotation_group="1019056846307478484acb0-ff52-4425-9497-
                     4681cab25c24urn:sequence:genbank:null"
                     /label="6,792 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6738..6757)
                     /created_by="primer3"
                     /%GC="60.0"
                     /Tm="60.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTCCCACATCTCCCCCTGAA"
                     /annotation_group="101905685395039347b8f1c-9d04-4b39-923a-
                     8eae491c80b2urn:sequence:genbank:null"
                     /label="6,796 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6742..6763)
                     /created_by="primer3"
                     /%GC="54.5"
                     /Tm="62.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AAAAACCTCCCACATCTCCCCC"
                     /annotation_group="10138259476891922f05024-6574-47e5-a791-
                     2a06ef21d295urn:sequence:genbank:null"
                     /label="6,802 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6743..6763)
                     /created_by="primer3"
                     /%GC="52.4"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AAAAACCTCCCACATCTCCCC"
                     /annotation_group="101382590734464cbfe1094-8be9-48c5-99a8-
                     45fc66f7e21curn:sequence:genbank:null"
                     /label="6,802 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6743..6762)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AAAACCTCCCACATCTCCCC"
                     /annotation_group="10190569092348786a47311-d083-4656-b0e7-
                     c0171f31ae2aurn:sequence:genbank:null"
                     /label="6,801 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6767..6792)
                     /created_by="primer3"
                     /%GC="38.5"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCACATTTGTAGAGGTTTTACTTGCT"
                     /annotation_group="101382592945363e87910dd-6dd2-4c2e-bb69-
                     b568caf4d90burn:sequence:genbank:null"
                     /label="6,831 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6768..6793)
                     /created_by="primer3"
                     /%GC="38.5"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACCACATTTGTAGAGGTTTTACTTGC"
                     /annotation_group="101382594006678ceace352-5732-48db-8192-
                     f83427ae16e7urn:sequence:genbank:null"
                     /label="6,832 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6768..6792)
                     /created_by="primer3"
                     /%GC="40.0"
                     /Tm="58.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCACATTTGTAGAGGTTTTACTTGC"
                     /annotation_group="101382593525420075871f2-bdf5-404b-a36a-
                     29e6b4a3473durn:sequence:genbank:null"
                     /label="6,831 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6804..6826)
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="60.2"
                     /Hairpin_Tm="45.4"
                     /Self_Dimer_Tm="4.1"
                     /Sequence="TAGCCATGCTCTAGGAAGATCCT"
                     /annotation_group="193371283560343d9e211f4-0e92-479e-8fac-
                     cd087b6700cdurn:sequence:genbank:null"
                     /label="6,865 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6805..6827)
                     /created_by="primer3"
                     /%GC="52.2"
                     /Tm="60.1"
                     /Hairpin_Tm="40.4"
                     /Self_Dimer_Tm="None"
                     /Sequence="GTAGCCATGCTCTAGGAAGATCC"
                     /annotation_group="1933712801719111e63fe30-9117-45a3-bd57-
                     caa4803eaf66urn:sequence:genbank:null"
                     /label="6,866 R"
                     /note="Geneious type: primer_bind_reverse"
     repeat_region   complement(6806..6995)
                     /label="ITR"
     misc_feature    complement(6809..6831)
                     /created_by="primer3"
                     /%GC="52.2"
                     /Tm="60.1"
                     /Hairpin_Tm="40.4"
                     /Self_Dimer_Tm="10.1"
                     /Sequence="CTACGTAGCCATGCTCTAGGAAG"
                     /annotation_group="193371281971322066bea7f-97d4-44cc-8cb4-
                     b3fa8a4f952eurn:sequence:genbank:null"
                     /label="6,870 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6825..6847)
                     /created_by="primer3"
                     /%GC="47.8"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCGCCATGCTACTTATCTACGTA"
                     /annotation_group="1933712843429445394fff9-6ab0-4c75-a6b6-
                     675880f19d6curn:sequence:genbank:null"
                     /label="6,886 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6833..6855)
                     /created_by="primer3"
                     /%GC="43.5"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGATTAACCCGCCATGCTACTTA"
                     /annotation_group="1933712827491213aae2900-5ff4-46ed-9398-
                     6623082dd98eurn:sequence:genbank:null"
                     /label="6,894 R"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     6931..6949
                     /created_by="primer3"
                     /%GC="68.4"
                     /Tm="63.6"
                     /Hairpin_Tm="43.1"
                     /Self_Dimer_Tm="13.8"
                     /Sequence="GACCAAAGGTCGCCCGACG"
                     /annotation_group="3828904955106621295100-1cfc-4d66-9d0c-d
                     93d94fcc5acurn:sequence:genbank:null"
                     /label="6,970 F"
     primer_bind     6968..6986
                     /created_by="primer3"
                     /%GC="68.4"
                     /Tm="64.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="13.3"
                     /Sequence="GGCCTCAGTGAGCGAGCGA"
                     /annotation_group="382890504010568f42b359-7be8-4726-a8bb-1
                     ff4cdbd6f0burn:sequence:genbank:null"
                     /label="7,007 F"
     primer_bind     6969..6987
                     /created_by="primer3"
                     /%GC="68.4"
                     /Tm="63.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="13.0"
                     /Sequence="GCCTCAGTGAGCGAGCGAG"
                     /annotation_group="38289047536071e7a2c8b7-fac7-4ee1-bd3c-8
                     3e3e8b6e68curn:sequence:genbank:null"
                     /label="7,008 F"
     primer_bind     6970..6988
                     /created_by="primer3"
                     /%GC="68.4"
                     /Tm="63.0"
                     /Hairpin_Tm="37.4"
                     /Self_Dimer_Tm="8.6"
                     /Sequence="CCTCAGTGAGCGAGCGAGC"
                     /annotation_group="382890370778711a3a5cf2-f681-4853-ac48-f
                     0d98995ce3burn:sequence:genbank:null"
                     /label="7,009 F"
     primer_bind     6971..6989
                     /created_by="primer3"
                     /%GC="68.4"
                     /Tm="63.3"
                     /Hairpin_Tm="43.0"
                     /Self_Dimer_Tm="7.3"
                     /Sequence="CTCAGTGAGCGAGCGAGCG"
                     /annotation_group="38289048594208118de50d-5587-4dd6-8f62-3
                     1e9c333dc54urn:sequence:genbank:null"
                     /label="7,010 F"
     misc_feature    complement(7023..7043)
                     /created_by="primer3"
                     /%GC="57.1"
                     /Tm="61.9"
                     /Hairpin_Tm="41.8"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAATACGCAAACCGCCTCTCC"
                     /annotation_group="6779252748768092b9eeda-d9c2-4f74-8a61-0
                     cffacbdad09urn:sequence:genbank:null"
                     /label="7,082 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7023..7042)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="60.7"
                     /Hairpin_Tm="41.8"
                     /Self_Dimer_Tm="None"
                     /Sequence="AATACGCAAACCGCCTCTCC"
                     /annotation_group="677925253652962a2b0e77-475d-4387-9089-4
                     487ffe61f3furn:sequence:genbank:null"
                     /label="7,081 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7024..7044)
                     /created_by="primer3"
                     /%GC="57.1"
                     /Tm="61.9"
                     /Hairpin_Tm="41.8"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCAATACGCAAACCGCCTCTC"
                     /annotation_group="677925265497939dc2712d-9ea9-4737-a3b9-d
                     ae1bc0a8bdaurn:sequence:genbank:null"
                     /label="7,083 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7024..7043)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="59.6"
                     /Hairpin_Tm="41.8"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAATACGCAAACCGCCTCTC"
                     /annotation_group="67792508488671e7ecadae-928d-42cb-be98-4
                     f291b723333urn:sequence:genbank:null"
                     /label="7,082 R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(7025..7044)
                     /created_by="primer3"
                     /%GC="55.0"
                     /Tm="61.0"
                     /Hairpin_Tm="41.8"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCAATACGCAAACCGCCTCT"
                     /annotation_group="677925285262785bd5fa72-b608-4ddf-b324-f
                     bb8f2835ad4urn:sequence:genbank:null"
                     /label="7,083 R"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gactcccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
     2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
     2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
     2641 gaagatcgta ggttaattaa ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     2701 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     2761 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2821 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2881 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     2941 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     3001 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     3061 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     3121 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     3181 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     3241 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     3301 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     3361 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     3421 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     3481 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3541 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3601 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     3661 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     3721 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     3781 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     3841 atccatcgat ccgcggccgc caccatgggt ccaaagaaga agcgcaaagt gggtgtgaag
     3901 atggccagca acttcactca gtttgttctg gttgacaacg gtggaacagg tgacgttaca
     3961 gtcgctccca gcaacttcgc aaacggcatc gctgagtgga tctccagcaa ttctaggagc
     4021 caggcttaca aagtgacttg ctctgtcaga caatcctccg cacagaatag gaagtacaca
     4081 atcaaggtcg aggtgccaaa gggagcatgg agatcctacc tgaacatgga gctgacaatc
     4141 cctatcttcg ccaccaactc cgattgcgaa ctgatcgtga aggccatgca gggtctgctg
     4201 aaagacggta atccaattcc ttctgccatc gctgccaact ccggtatcta tgcaatggcc
     4261 tccaacttca cccagttcgt gctggttgac aatggaggca caggcgacgt gacagtggca
     4321 ccatctaact ttgccaatgg tatcgctgaa tggatttcta gcaacagcag gtcccaggca
     4381 tacaaggtga cctgctccgt caggcaatcc tccgctcaga atcgcaagta taccatcaag
     4441 gtggaggtcc ctaaaggtgc ttggaggagc tatctgaaca tggagctgac tatccctatc
     4501 tttgcaacta actctgactg cgagctgatt gtcaaggcaa tgcagggact gctgaaggac
     4561 ggtaatccca ttccttctgc catcgcagcc aattccggag gtggttctgg aggaactggt
     4621 ggctctggtg gtacccagct gcatttaccg caggttttag ctgacgctgt ctcacgcctg
     4681 gtcctgggta agtttggtga cctgaccgac aacttctcct cccctcacgc tcgcagaaaa
     4741 gtgctggctg gagtcgtcat gacaacaggc acagatgtta aagatgccaa ggtgataagt
     4801 gtttctacag gaacaaaatg tattaatggt gaatacatga gtgatcgtgg ccttgcatta
     4861 aatgactgcc atgcagaaat aatatctcgg agatccttgc tcagatttct ttatacacaa
     4921 cttgagcttt acttaaataa caaagatgat caaaaaagat ccatctttca gaaatcagag
     4981 cgaggggggt ttaggctgaa ggagaatgtc cagtttcatc tgtacatcag cacctctccc
     5041 tgtggagatg ccagaatctt ctcaccacat gagccaatcc tggaagaacc agcagataga
     5101 cacccaaatc gtaaagcaag aggacagcta cggaccaaaa tagagtctgg tcagggggcg
     5161 attccagtgc gctccaatgc gagcatccaa acgtgggacg gggtgctgca aggggagcgg
     5221 ctgctcacca tgtcctgcag tgacaagatt gcacgctgga acgtggtggg catccaggga
     5281 tcactgctca gcattttcgt ggagcccatt tacttctcga gcatcatcct gggcagcctt
     5341 taccacgggg accacctttc cagggccatg taccagcgga tctccaacat agaggacctg
     5401 ccacctctct acaccctcaa caagcctttg ctcagtggca tcagcaatgc agaagcacgg
     5461 cagccaggga aggcccccaa cttcagtgtc aactggacgg taggcgactc cgctattgag
     5521 gtcatcaacg ccacgactgg gaaggatgag ctgggccgcg cgtcccgcct gtgtaagcac
     5581 gcgttgtact gtcgctggat gcgtgtgcac ggcaaggttc cctcccactt actacgctcc
     5641 aagattacca agcccaacgt gtaccatgag tccaagctgg cggcaaagga gtaccaggcc
     5701 gccaaggcgc gtctgttcac agccttcatc aaggcggggc tgggggcctg ggtggagaag
     5761 cccaccgagc aggaccagtt ctcactcacg taagcggccg ctcgagtcta gagggcccgt
     5821 ttaaacccct agtgatatct ccagaggatc atatcgataa tcaacctctg gattacaaaa
     5881 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5941 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6001 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6061 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6121 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6181 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6241 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     6301 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6361 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6421 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acccgggcgg ccgctcataa
     6481 agctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6541 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6601 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6661 gaggattggg aagacaatag caggcatgct ggggatgcgg tccggactgt acaattgcat
     6721 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     6781 ctacaaatgt ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc
     6841 atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc
     6901 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     6961 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcat taatgaatcg gccaacgcgc
     7021 ggggagaggc ggtttgcgta ttgggcgct
//
