LOCUS pcDNA3_mId1 5854 bp DNA circular 08-JUN-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5446) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 28, 2018 from SnapGene Viewer 4.2.6 http://www.snapgene.com COMMENT Created: Saturday, December 27, 2008 1:53 PM pcDNA3 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 220..874 /note="CMV promoter" /locus_tag="CMV promoter" /label="CMV promoter" /ApEinfo_label="CMV promoter" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 875..893 /note="T7" /locus_tag="T7" /label="T7" /ApEinfo_label="T7" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 1418..1435 /note="Sp6" /locus_tag="Sp6" /label="Sp6" /ApEinfo_label="Sp6" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 1437..1668 /note="bGH pA" /locus_tag="bGH pA" /label="bGH pA" /ApEinfo_label="bGH pA" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 2209..2534 /note="SV40 promoter" /locus_tag="SV40 promoter" /label="SV40 promoter" /ApEinfo_label="SV40 promoter" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 2570..3364 /note="Neo ORF" /locus_tag="Neo ORF" /label="Neo ORF" /ApEinfo_label="Neo ORF" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 3419..3791 /note="SV40 pA" /locus_tag="SV40 pA" /label="SV40 pA" /ApEinfo_label="SV40 pA" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 4051..4724 /note="ColE1" /locus_tag="ColE1" /label="ColE1" /ApEinfo_label="ColE1" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 4869..5729 /note="AmpR" /locus_tag="AmpR" /label="AmpR" /ApEinfo_label="AmpR" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" misc_feature 918..1364 /locus_tag="Mouse Id1" /label="Mouse Id1" /ApEinfo_label="Mouse Id1" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0 -0.5} {} 0} width 5 offset 0" ORIGIN 1 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat 61 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc 121 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat 181 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac 241 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 301 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 361 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 421 agggactttc cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt 481 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 541 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 601 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 661 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 721 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 781 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac 841 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca 901 agcttgatat cgaattcATG AAGGTCGCCA GTGGCAGTGC CGCAGCCGCT GCAGGCCCTA 961 GCTGTTCGCT GAAGGCGGGC AGGACAGCGG GCGAGGTGGT ACTTGGTCTG TCGGAGCAAA 1021 GCGTGGCCAT CTCGCGCTGC GCTGGGACGC GCCTGCCCGC CTTGCTGGAC GAGCAGCAGG 1081 TGAACGTCCT GCTCTACGAC ATGAACGGCT GCTACTCACG CCTCAAGGAG CTGGTGCCCA 1141 CCCTGCCCCA GAACCGCAAA GTGAGCAAGG TGGAGATCCT GCAGCATGTA ATCGACTACA 1201 TCAGGGACCT GCAGCTGGAG CTGAACTCGG AGTCTGAAGT CGGGACCACC GGAGGCCGGG 1261 GACTGCCTGT CCGCGCCCCG CTCAGCACCC TGAACGGCGA GATCAGTGCC TTGGCGGCCG 1321 AGGCGGCATG TGTTCCAGCC GACGATCGCA TCTTGTGTCG CTGAggatcc actagttcta 1381 gagcggccgc tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag 1441 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 1501 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 1561 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 1621 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc 1681 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg 1741 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 1801 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 1861 ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg 1921 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 1981 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 2041 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 2101 ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta 2161 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag 2221 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 2281 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 2341 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 2401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca 2461 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg 2521 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca 2581 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 2641 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 2701 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc 2761 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 2821 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 2881 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 2941 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 3001 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 3061 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct 3121 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 3181 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 3241 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 3301 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 3361 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 3421 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 3481 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 3541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 3601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 3661 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 3721 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 3781 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 3841 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 3901 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 3961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 4021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 4081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 4141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 4201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 4261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg 4321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 4381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 4441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 4501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 4561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 4621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 4681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 4741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 4801 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 4861 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 4921 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 4981 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 5041 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 5101 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 5161 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 5221 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 5281 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 5341 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 5401 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 5461 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 5521 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 5581 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 5641 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 5701 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 5761 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 5821 ataggggttc cgcgcacatt tccccgaaaa gtgc //