LOCUS       Exported                5185 bp ds-DNA     circular SYN 28-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 28, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA
[LOCUS]: Exported
[ACCESSION]: 
            .
[SOURCE]: 1..5185
[CDS]: 178..1203
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..173
                     /label=ConLS'
     CDS             178..1203
                     /label=GFP drop-out
     misc_feature    1208..1397
                     /label=ConRE'
     misc_feature    1402..3015
                     /label=hphMX cassette
                     /note="label=hphMX cassette - 1402..3015"
     rep_origin      3020..3410
                     /label=KmARS/CEN7
                     /note="Minimal/synthetic ARS/CEN from K.marxianus 
                     DMKU3-1042"
     misc_feature    complement(3415..5185)
                     /label=kanR-ColE1
ORIGIN
        1 ccctgaattc gcatctagac tgatgagacg tggtagagcc acaaacagcc ggtacaagca
       61 acgatctcca ggaccatctg aatcatgcgc ggatgacacg aactcacgac ggcgatcaca
      121 gacattaacc cacagtacag acactgcgac aacgtggcaa ttcgtcgcaa tacaacgtga
      181 gaccgaaagt gaaacgtgat ttcatgcgtc attttgaaca ttttgtaaat cttatttaat
      241 aatgtgtgcg gcaattcaca tttaatttat gaatgttttc ttaacatcgc ggcaactcaa
      301 gaaacggcag gttcggatct tagctactag agaaagagga gaaatactag atgcgtaaag
      361 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg
      421 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc
      481 tgaagttcat ctgtactact ggtaaactgc cggttccttg gccgactctg gtaacgacgc
      541 tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct
      601 tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg
      661 gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg
      721 agctgaaagg cattgacttt aaagaggacg gcaatatcct gggccataag ctggaataca
      781 attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga
      841 attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc
      901 aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc
      961 aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg
     1021 taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgacca ggcatcaaat
     1081 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1141 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggtc
     1201 tcagctggaa atctgctcgt cagtggtgct cacactgacg aatcatgtac agatcatacc
     1261 gatgactgcc tggcgactca caactaagca agacagccgg aaccagcgcc ggcgaacacc
     1321 actgcatata tggcatatca caacagtcca cgtctcaagc agttacagag atgttacgaa
     1381 ccactagtgc actgcagtac agtttagctt gcctcgtccc cgccgggtca cccggccagc
     1441 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
     1501 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
     1561 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctcca gacctgcgag
     1621 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
     1681 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
     1741 ttgctaggat acagttctca catcacatcc gaacataaac aaaaatgggt aaaaagcctg
     1801 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtgtccgacc
     1861 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     1921 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     1981 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaatttagcg
     2041 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     2101 aaaccgaact gcccgctgtt ctgcaaccgg tcgcggaggc catggatgcg atcgctgcgg
     2161 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     2221 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     2281 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     2341 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     2401 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     2461 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     2521 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     2581 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     2641 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     2701 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     2761 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taaagtaact
     2821 gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta
     2881 gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc
     2941 tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg
     3001 ctggtcgcta tactggagtt tctacgcaag acttcttgaa gtgaaaacca actttcagtc
     3061 ttcaaactaa aaatgaaaat cagtgggcta aattcagcat aatacctgtg gatataatta
     3121 catacaaaaa tataattaaa aatatacgtt aaaaataatt tatttttgta aaaactataa
     3181 aatatatttt actttcggaa caacttttta acttataatt ttgttttaaa taaaaacatt
     3241 gtatttaaaa ataataaatt attaaataaa attttaaagt atgtttattt aaaatataga
     3301 atactattaa aattacttta aactttaaaa taaaaaaata ataaattata tagttttaaa
     3361 attaataaat tttatacacg tgaccagatc ataatagttt tctttccttg ccgagcggcc
     3421 gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
     3481 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     3541 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     3601 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     3661 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     3721 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     3781 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg
     3841 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     3901 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     3961 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     4021 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     4081 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     4141 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     4201 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     4261 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     4321 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga
     4381 ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct taacgtgagt
     4441 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     4501 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4561 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4621 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4681 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4741 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4801 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4861 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4921 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4981 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5041 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5101 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5161 attctgtgga taaccgtgcg gccgc
//
