LOCUS       Exported                5879 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..894
                     /label=I1L
                     /note="Left homology arm targeting the LAC4 locus"
     misc_feature    895..920
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             925..1950
                     /codon_start=1
                     /label=GFP drop-out
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1955..2144
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2149..3207
                     /label=ScURA3
     misc_feature    3211..4088
                     /label=I1R
                     /note="Right homology arm targeting the LAC4 locus"
     misc_feature    complement(4089..4096)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4100..5879)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgccgaaa gtgattgaag aaccctcaaa cttgctggat atttccttac
       61 ccttgacctt taggctttca attttaccca acaatttgtc caagacaaat tgcaatccac
      121 tggattcaac tgagacataa cgtttaccgt cgttgatctt ggtaggtttt tctgctgtct
      181 ctgtaacaaa atctggtacc tttaatggaa gttcggcttg gccccaggca atttcatgac
      241 ctgcctttag aacaccagca tcatccttta acacggcaac aacataagtg gtatcagaag
      301 gaatagtaac agattcttct ggctttaaag ctggaacgtc gattgtcttt cccgtgtcct
      361 tgtcgataaa caataagtga tctgtcgtaa tgaagtcgtg cttatttgtg attgttacag
      421 atccgtgcgc aattttaata tgaacgggtt caataacctt cttatactct acaaggcccg
      481 gagtaggatt atgctcactg ttacacaaac catccatgat gaacactccg tcatgaacct
      541 cttccttaaa gtcaccacca taagcataag ctttatgcaa cttaccatct gcagtactaa
      601 catcttcgaa ttcaataccg tgatttgccc attcccagat aaagccacct tggtaaaact
      661 tctccttgta gaacaactct tgatattctt tcaaagaacc cgggccgtta cccattgcat
      721 gaccgtactc acacaagatc aaaggctttt caaacttacc attttcatca gtgtggttct
      781 tcctccacct ttccataatt tcaaatgttg ggtacatgaa actaaagata tctgcactca
      841 aagcgttcaa gtcaccctca taatgcacca gtctggtagg atccaattgc ttaagaattc
      901 gcatctagac tgatgagacg aacgtgagac cgaaagtgaa acgtgatttc atgcgtcatt
      961 ttgaacattt tgtaaatctt atttaataat gtgtgcggca attcacattt aatttatgaa
     1021 tgttttctta acatcgcggc aactcaagaa acggcaggtt cggatcttag ctactagaga
     1081 aagaggagaa atactagatg cgtaaaggcg aagagctgtt cactggtgtc gtccctattc
     1141 tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag
     1201 gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt aaactgccgg
     1261 ttccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt gctcgttatc
     1321 cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc tatgtgcagg
     1381 aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg
     1441 aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa gaggacggca
     1501 atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac atcaccgccg
     1561 ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca
     1621 gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt cctgttctgc
     1681 tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg aacgagaaac
     1741 gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat ggtatggatg
     1801 aactgtacaa atgaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1861 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1921 gggtgggcct ttctgcgttt ataggtctca gctggaaatc tgctcgtcag tggtgctcac
     1981 actgacgaat catgtacaga tcataccgat gactgcctgg cgactcacaa ctaagcaaga
     2041 cagccggaac cagcgccggc gaacaccact gcatatatgg catatcacaa cagtccacgt
     2101 ctcaagcagt tacagagatg ttacgaacca ctagtgcact gcagtacacg gtttccttga
     2161 aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt
     2221 agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta
     2281 acccaactgc acagaacaaa aacgtgcagg aaacgaagat aaatcatgtc gaaagctaca
     2341 tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
     2401 cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
     2461 gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
     2521 actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
     2581 tttttactct tcgaggacag aaaatttgct gacattggta atacagtcaa attgcagtac
     2641 tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
     2701 ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga
     2761 ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact
     2821 aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
     2881 agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
     2941 ttagatgaca agggagatgc attgggtcaa cagtatagaa ccgtggatga tgtggtttct
     3001 acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
     3061 gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
     3121 caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct
     3181 tcaatttaat tatatcagtt attacccgag tgcttaatta gcttgtacat ggctttgtgg
     3241 tttctgccat aacaagcttc gttacccaag gaccagataa taatcgaagg atgattgaca
     3301 tctcttagga caagttggga agctctgtct aagtacgcga cctcgtactc tggattatct
     3361 gataagtaat gggcattaac atcgtagagt ttatttttgg tatctggata ttcagcctcc
     3421 aagttcgtat gacgattaaa tggctcttga acaccgtgag tttcaagatc tgcctcatca
     3481 atgacccaga agcctagttt atcgaagagg tcatacacct taggatggtt tggataatgc
     3541 gagttacgaa cagcattgat gttaaacttc ttcattagaa tcaagtctct aacaacaaaa
     3601 tctaatggca cagctctacc gaaccttgga tggtgatcat gcctgttgac acctctaaag
     3661 agaatgtctt tgccattaac agtaatgtta ccgtccttca actccacttg tctgaaacca
     3721 acatggtgct taatagattg aatcacactg ccatcagatc caattaaatc caactggtac
     3781 ttgtacaaag taggattttc tgcggtccaa tgttctgggg ccttgacgtt gatcttgaaa
     3841 gctgtttctt cgtccttttt ggtcgaaaag gaaataaatt ctttagttga aaacgtcgtg
     3901 ttcccattct cctcgttcaa caaagagctt gcatcgtaaa ctttagatcc atcttcaggt
     3961 tcgtaaagtg tgaaattgat gtgatcataa gaagaaccct ggacatcaac tttcacagaa
     4021 agctctgcat cctgatactg agagtccaca aaagttgtag tgaccctaac gtcttcaata
     4081 tgggccttgg cgcgcctcag ccgaccgagc ggccgcgtgt tacaaccaat taaccaattc
     4141 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     4201 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     4261 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     4321 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     4381 gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg
     4441 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     4501 ttgcgcctga gcgaggcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     4561 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     4621 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     4681 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     4741 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     4801 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     4861 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     4921 cggcctggag caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt
     4981 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca
     5041 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa
     5101 ggatcagtca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tgcggccgc
//
