LOCUS       Exported                6211 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..894
                     /label=I1L
     misc_feature    895..920
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             925..1950
                     /codon_start=1
                     
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1955..2144
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2149..3539
                     /label=KanMX
                     /note="label=kanMX - 2141..3531"
     misc_feature    3543..4420
                     /label=I1R
                     /note="label=I5R - 3536..4385"
     misc_feature    complement(4421..4428)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4432..6211)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgccgaaa gtgattgaag aaccctcaaa cttgctggat atttccttac
       61 ccttgacctt taggctttca attttaccca acaatttgtc caagacaaat tgcaatccac
      121 tggattcaac tgagacataa cgtttaccgt cgttgatctt ggtaggtttt tctgctgtct
      181 ctgtaacaaa atctggtacc tttaatggaa gttcggcttg gccccaggca atttcatgac
      241 ctgcctttag aacaccagca tcatccttta acacggcaac aacataagtg gtatcagaag
      301 gaatagtaac agattcttct ggctttaaag ctggaacgtc gattgtcttt cccgtgtcct
      361 tgtcgataaa caataagtga tctgtcgtaa tgaagtcgtg cttatttgtg attgttacag
      421 atccgtgcgc aattttaata tgaacgggtt caataacctt cttatactct acaaggcccg
      481 gagtaggatt atgctcactg ttacacaaac catccatgat gaacactccg tcatgaacct
      541 cttccttaaa gtcaccacca taagcataag ctttatgcaa cttaccatct gcagtactaa
      601 catcttcgaa ttcaataccg tgatttgccc attcccagat aaagccacct tggtaaaact
      661 tctccttgta gaacaactct tgatattctt tcaaagaacc cgggccgtta cccattgcat
      721 gaccgtactc acacaagatc aaaggctttt caaacttacc attttcatca gtgtggttct
      781 tcctccacct ttccataatt tcaaatgttg ggtacatgaa actaaagata tctgcactca
      841 aagcgttcaa gtcaccctca taatgcacca gtctggtagg atccaattgc ttaagaattc
      901 gcatctagac tgatgagacg aacgtgagac cgaaagtgaa acgtgatttc atgcgtcatt
      961 ttgaacattt tgtaaatctt atttaataat gtgtgcggca attcacattt aatttatgaa
     1021 tgttttctta acatcgcggc aactcaagaa acggcaggtt cggatcttag ctactagaga
     1081 aagaggagaa atactagatg cgtaaaggcg aagagctgtt cactggtgtc gtccctattc
     1141 tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag
     1201 gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt aaactgccgg
     1261 ttccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt gctcgttatc
     1321 cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc tatgtgcagg
     1381 aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg
     1441 aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa gaggacggca
     1501 atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac atcaccgccg
     1561 ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca
     1621 gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt cctgttctgc
     1681 tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg aacgagaaac
     1741 gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat ggtatggatg
     1801 aactgtacaa atgaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1861 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1921 gggtgggcct ttctgcgttt ataggtctca gctggaaatc tgctcgtcag tggtgctcac
     1981 actgacgaat catgtacaga tcataccgat gactgcctgg cgactcacaa ctaagcaaga
     2041 cagccggaac cagcgccggc gaacaccact gcatatatgg catatcacaa cagtccacgt
     2101 ctcaagcagt tacagagatg ttacgaacca ctagtgcact gcagtacaag cttgcctcgt
     2161 ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg
     2221 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
     2281 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
     2341 gctcctcgct ccagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
     2401 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
     2461 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
     2521 aacaaaaatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga
     2581 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     2641 ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     2701 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     2761 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     2821 gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     2881 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     2941 ttttaacagc gatcgcgtat ttcgtctggc tcaggcgcaa tcacgaatga ataacggttt
     3001 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     3061 agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     3121 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     3181 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3241 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3301 attgcagttt catttgatgc tcgatgagtt tttctaaagt aactgacaat aaaaagattc
     3361 ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat
     3421 caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt
     3481 taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgg
     3541 agtgcttaat tagcttgtac atggctttgt ggtttctgcc ataacaagct tcgttaccca
     3601 aggaccagat aataatcgaa ggatgattga catctcttag gacaagttgg gaagctctgt
     3661 ctaagtacgc gacctcgtac tctggattat ctgataagta atgggcatta acatcgtaga
     3721 gtttattttt ggtatctgga tattcagcct ccaagttcgt atgacgatta aatggctctt
     3781 gaacaccgtg agtttcaaga tctgcctcat caatgaccca gaagcctagt ttatcgaaga
     3841 ggtcatacac cttaggatgg tttggataat gcgagttacg aacagcattg atgttaaact
     3901 tcttcattag aatcaagtct ctaacaacaa aatctaatgg cacagctcta ccgaaccttg
     3961 gatggtgatc atgcctgttg acacctctaa agagaatgtc tttgccatta acagtaatgt
     4021 taccgtcctt caactccact tgtctgaaac caacatggtg cttaatagat tgaatcacac
     4081 tgccatcaga tccaattaaa tccaactggt acttgtacaa agtaggattt tctgcggtcc
     4141 aatgttctgg ggccttgacg ttgatcttga aagctgtttc ttcgtccttt ttggtcgaaa
     4201 aggaaataaa ttctttagtt gaaaacgtcg tgttcccatt ctcctcgttc aacaaagagc
     4261 ttgcatcgta aactttagat ccatcttcag gttcgtaaag tgtgaaattg atgtgatcat
     4321 aagaagaacc ctggacatca actttcacag aaagctctgc atcctgatac tgagagtcca
     4381 caaaagttgt agtgacccta acgtcttcaa tatgggcctt ggcgcgcctc agccgaccga
     4441 gcggccgcgt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
     4501 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     4561 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     4621 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     4681 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
     4741 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     4801 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg aaatacgcga
     4861 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     4921 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     4981 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     5041 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     5101 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     5161 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     5221 tataaatcag catccatgtt ggaatttaat cgcggcctgg agcaagacgt ttcccgttga
     5281 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     5341 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     5401 tgttgaataa atcgaacttt tgctgagttg aaggatcagt catgaccaaa atcccttaac
     5461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5641 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6181 cccctgattc tgtggataac cgtgcggccg c
//
