LOCUS       Exported                6121 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..1015
                     /label=I3L
     misc_feature    1016..1041
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             1046..2071
                     /codon_start=1
                     /label=GFP dropout
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    2076..2265
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2270..3326
                     /label=URA3
     misc_feature    3332..4333
                     /label=I3R
     misc_feature    complement(4335..4342)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4351..6121)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgccaccg aatattaaat aatctttttt tatttcttat gtacgcctgc
       61 tttgtatgat acaaaatcat cgtatcgtat tcggcttcat gttatccgtc gaatttagat
      121 taccttggct aaggggccaa ggtggatgat tcgtatgagt atcacaattt acaaattaca
      181 agttttgcaa agagtatgta aactgtaaaa aagaaaacat tgacaaatat acctgaaaag
      241 agtccatttt ctacatttga gaaaaaaagg aataaagtga ttctcctcat cgctcataaa
      301 aaaatactat tcatgagcaa aattgagaaa aatggcaatc acattcaata ctagttagat
      361 agccatatct aaactgctgt acgtggttta atactagcac caacatcatt tgaataatgg
      421 cgaagaaacc aatttcgaaa cattcccgtg cggcaagaag atcagaagca gccgaaccgg
      481 aggctaaggc tttagagact ttacccagag ctgaaaagac agattttgca gcaacattaa
      541 tcagaactgc taacaaaaac gaagctttgc tcgaagcgaa gatccaaaag cgtggacaaa
      601 aaaataagaa gaaaaacgcg ggaagagtga gtaagcaact tatagatgac agttctttaa
      661 caagtaggaa agctattcgt gccttaaata ttaccagtag gctcgatggt aagagggaga
      721 aagccattca tagagctaaa tatgtgcagt cagctcgtaa agctggatgg gatactacaa
      781 accaaagcat taggaaagaa ttatcgacac tacaaaacgc agcaaatacg gtcagcgatt
      841 ccgttaacaa aagcacggac aaggaagggt ctgcgaatac tgaagatgcg atgatagaag
      901 acgatcaaga tcacattgaa atttacgaca gtgctaaaag ccagaaggta caagaaaaag
      961 cagtatcaac cacttttacg gcatcaaacg tatttacgtc attagaggat gagatgaatt
     1021 cgcatctaga ctgatgagac gaacgtgaga ccgaaagtga aacgtgattt catgcgtcat
     1081 tttgaacatt ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttatga
     1141 atgttttctt aacatcgcgg caactcaaga aacggcaggt tcggatctta gctactagag
     1201 aaagaggaga aatactagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
     1261 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
     1321 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
     1381 gttccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
     1441 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
     1501 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
     1561 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaggacggc
     1621 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
     1681 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
     1741 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
     1801 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
     1861 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
     1921 gaactgtaca aatgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1981 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2041 cgggtgggcc tttctgcgtt tataggtctc agctggaaat ctgctcgtca gtggtgctca
     2101 cactgacgaa tcatgtacag atcataccga tgactgcctg gcgactcaca actaagcaag
     2161 acagccggaa ccagcgccgg cgaacaccac tgcatatatg gcatatcaca acagtccacg
     2221 tctcaagcag ttacagagat gttacgaacc actagtgcac tgcagtacac ggtttccttg
     2281 aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact
     2341 tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt
     2401 aacccaactg cacagaacaa aaacgtgcag gaaacgaaga taaatcatgt cgaaagctac
     2461 atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat
     2521 gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact
     2581 ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt
     2641 gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa
     2701 ttttttactc ttcgaggaca gaaaatttgc tgacattggt aatacagtca aattgcagta
     2761 ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt
     2821 gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag
     2881 aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac
     2941 taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca
     3001 aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg
     3061 tttagatgac aagggagatg cattgggtca acagtataga accgtggatg atgtggtttc
     3121 tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa
     3181 ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca
     3241 gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc
     3301 ttcaatttaa ttatatcagt tattacccga gttgaagcat agaagaaatg tttaaccaaa
     3361 aactatcctt tttatttatt ttgaattttc tctctcatat ttttgtaaaa cgatacttta
     3421 accacactca agcttttgct tgctactcaa atacctgaaa tctttataga caattttcct
     3481 tcttattcgt aataataaaa ctatattgtt ctcttcctgt tttttttttt aaaaaaaaaa
     3541 actgtcaaaa tctcccccac agaaaaataa aaacaagtta tacttttggg agtaaaccat
     3601 gaacttaaaa caacagttca tattcttaag tagtgccact atagttaacc agagacaatt
     3661 taactcctaa atcttcatac tgaagattga cataaactaa ataatgacta cactgttact
     3721 tttgagtcag ttgggtcttt tggcattcgc caatgctatt aaccctatgg taaagggcga
     3781 atatagaatt gaacatccta agataggaga cttgtcagaa attgaatctg ctatctctcc
     3841 caaatctttc tatgaatcat ttcaatatgg ttccgaagcg gagttccgta agaattgggt
     3901 ttcttctgat ctaacaatgg aacatgtaga cgaaacatct tcgaaggcgt tccctggcac
     3961 atggaagttg cagtcaccat ttttggtccc aggattcagt gatgatctat ctttagtttt
     4021 gggtagcgaa caaactagag ctgctattgc ccataggtta aaatacgatc tatcgatcaa
     4081 ggataatgag aaactagtgg tgcaatatga agttaaactt caaaaaatgc ttgaatgtgg
     4141 cggtgcatat atcaaattgt tgaaaacctc cgcaactgat ctttctcagt acgaccactc
     4201 gtcagatgac tatgctgtca tctttggccc agacttttgc caaggttatg tggacgaaat
     4261 tcatttggtt ttgaagcgcg agaatccttt ttctggaaaa tctgaagaca aattcttatt
     4321 aaatgcccca aaacggcgcg cctcagccga gcggccgcgt gttacaacca attaaccaat
     4381 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     4441 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     4501 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     4561 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     4621 acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca
     4681 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     4741 gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     4801 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     4861 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
     4921 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     4981 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     5041 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     5101 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     5161 cgcggcctgg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
     5221 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
     5281 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
     5341 aaggatcagt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5401 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5461 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5521 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5581 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5641 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5701 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5761 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5821 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5881 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5941 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6001 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6061 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtgcggccg
     6121 c
//
