LOCUS       Exported                6172 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..1015
                     /label=I3L
     misc_feature    1016..1041
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             1046..2071
                     /codon_start=1
                     /label=GFP dropout
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    2076..2265
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2270..3379
                     /label=ScHIS3
                     /note="label=kanMX - 2141..3531"
     misc_feature    3384..4385
                     /label=I3R
                     /note="label=I5R - 3536..4385"
     misc_feature    complement(4386..4393)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4402..6172)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgccaccg aatattaaat aatctttttt tatttcttat gtacgcctgc
       61 tttgtatgat acaaaatcat cgtatcgtat tcggcttcat gttatccgtc gaatttagat
      121 taccttggct aaggggccaa ggtggatgat tcgtatgagt atcacaattt acaaattaca
      181 agttttgcaa agagtatgta aactgtaaaa aagaaaacat tgacaaatat acctgaaaag
      241 agtccatttt ctacatttga gaaaaaaagg aataaagtga ttctcctcat cgctcataaa
      301 aaaatactat tcatgagcaa aattgagaaa aatggcaatc acattcaata ctagttagat
      361 agccatatct aaactgctgt acgtggttta atactagcac caacatcatt tgaataatgg
      421 cgaagaaacc aatttcgaaa cattcccgtg cggcaagaag atcagaagca gccgaaccgg
      481 aggctaaggc tttagagact ttacccagag ctgaaaagac agattttgca gcaacattaa
      541 tcagaactgc taacaaaaac gaagctttgc tcgaagcgaa gatccaaaag cgtggacaaa
      601 aaaataagaa gaaaaacgcg ggaagagtga gtaagcaact tatagatgac agttctttaa
      661 caagtaggaa agctattcgt gccttaaata ttaccagtag gctcgatggt aagagggaga
      721 aagccattca tagagctaaa tatgtgcagt cagctcgtaa agctggatgg gatactacaa
      781 accaaagcat taggaaagaa ttatcgacac tacaaaacgc agcaaatacg gtcagcgatt
      841 ccgttaacaa aagcacggac aaggaagggt ctgcgaatac tgaagatgcg atgatagaag
      901 acgatcaaga tcacattgaa atttacgaca gtgctaaaag ccagaaggta caagaaaaag
      961 cagtatcaac cacttttacg gcatcaaacg tatttacgtc attagaggat gagatgaatt
     1021 cgcatctaga ctgatgagac gaacgtgaga ccgaaagtga aacgtgattt catgcgtcat
     1081 tttgaacatt ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttatga
     1141 atgttttctt aacatcgcgg caactcaaga aacggcaggt tcggatctta gctactagag
     1201 aaagaggaga aatactagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
     1261 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
     1321 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
     1381 gttccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
     1441 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
     1501 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
     1561 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaggacggc
     1621 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
     1681 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
     1741 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
     1801 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
     1861 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
     1921 gaactgtaca aatgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1981 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2041 cgggtgggcc tttctgcgtt tataggtctc agctggaaat ctgctcgtca gtggtgctca
     2101 cactgacgaa tcatgtacag atcataccga tgactgcctg gcgactcaca actaagcaag
     2161 acagccggaa ccagcgccgg cgaacaccac tgcatatatg gcatatcaca acagtccacg
     2221 tctcaagcag ttacagagat gttacgaacc actagtgcac tgcagtacaa acacagtcct
     2281 ttcccgcaat tttctttttc tattactctt ggcctcctct agtacactct atattttttt
     2341 atgcctcggt aatgattttc attttttttt ttccacctag cggatgactc tttttttttc
     2401 ttagcgattg gcattatcac ataatgaatt atacattata taaagtaatg tgatttcttc
     2461 gaagaatata ctaaaaaatg agcaggcaag ataaacgaag gcaaagatga cagagcagaa
     2521 agccctagta aagcgtatta caaatgaaac caagattcag attgcgatct ctttaaaggg
     2581 tggtccccta gcgatagagc actcgatctt cccagaaaaa gaggcagaag cagtagcaga
     2641 acaggccaca caatcgcaag tgattaacgt ccacacaggt atagggtttc tggaccatat
     2701 gatacatgct ctggccaagc attccggctg gtcgctaatc gttgagtgca ttggtgactt
     2761 acacatagac gaccatcaca ccactgagga ctgcgggatt gctctcggtc aagcttttaa
     2821 agaggcccta ggggccgtgc gtggagtaaa aaggtttgga tcaggatttg cgcctttgga
     2881 tgaggcactt tccagagcgg tggttgatct ttcgaacagg ccgtacgcag ttgtcgaact
     2941 tggtttgcaa agggagaaag taggtgatct ctcttgcgag atgatcccgc attttcttga
     3001 aagctttgca gaggctagca gaattaccct ccacgttgat tgtctgcgag gcaagaatga
     3061 tcatcaccgt agtgagagtg cgttcaaggc tcttgcggtt gccataagag aagccacctc
     3121 gcccaatggt accaacgatg ttccctccac caaaggtgtt cttatgtagt gacaccgatt
     3181 atttaaagct gctgcatacg atatatatac atgtgtatat atgtatacct atgaatgtca
     3241 gtaagtatgt atacgaacag tatgatactg aagatgacaa ggtaatgcat cattctatac
     3301 gtgtcattct gaacgaggcg cgctttcctt ttttcttttt gctttttctt tttttttctc
     3361 ttgaactcga cggatcatag agttgaagca tagaagaaat gtttaaccaa aaactatcct
     3421 ttttatttat tttgaatttt ctctctcata tttttgtaaa acgatacttt aaccacactc
     3481 aagcttttgc ttgctactca aatacctgaa atctttatag acaattttcc ttcttattcg
     3541 taataataaa actatattgt tctcttcctg tttttttttt taaaaaaaaa aactgtcaaa
     3601 atctccccca cagaaaaata aaaacaagtt atacttttgg gagtaaacca tgaacttaaa
     3661 acaacagttc atattcttaa gtagtgccac tatagttaac cagagacaat ttaactccta
     3721 aatcttcata ctgaagattg acataaacta aataatgact acactgttac ttttgagtca
     3781 gttgggtctt ttggcattcg ccaatgctat taaccctatg gtaaagggcg aatatagaat
     3841 tgaacatcct aagataggag acttgtcaga aattgaatct gctatctctc ccaaatcttt
     3901 ctatgaatca tttcaatatg gttccgaagc ggagttccgt aagaattggg tttcttctga
     3961 tctaacaatg gaacatgtag acgaaacatc ttcgaaggcg ttccctggca catggaagtt
     4021 gcagtcacca tttttggtcc caggattcag tgatgatcta tctttagttt tgggtagcga
     4081 acaaactaga gctgctattg cccataggtt aaaatacgat ctatcgatca aggataatga
     4141 gaaactagtg gtgcaatatg aagttaaact tcaaaaaatg cttgaatgtg gcggtgcata
     4201 tatcaaattg ttgaaaacct ccgcaactga tctttctcag tacgaccact cgtcagatga
     4261 ctatgctgtc atctttggcc cagacttttg ccaaggttat gtggacgaaa ttcatttggt
     4321 tttgaagcgc gagaatcctt tttctggaaa atctgaagac aaattcttat taaatgcccc
     4381 aaaacggcgc gcctcagccg agcggccgcg tgttacaacc aattaaccaa ttctgattag
     4441 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     4501 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     4561 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     4621 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     4681 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     4741 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     4801 tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     4861 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     4921 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     4981 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     5041 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     5101 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     5161 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctg
     5221 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     5281 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     5341 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     5401 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5461 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5521 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5581 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5641 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5701 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5761 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5821 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5881 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5941 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6001 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6061 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6121 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc gc
//
