LOCUS       Exported                6979 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..1015
                     /label=I3L
     misc_feature    1016..1041
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             1046..2071
                     /codon_start=1
                     /label=GFP dropout
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    2076..2265
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2270..4186
                     /label=AgLEU2
                     /note="label=kanMX - 2141..3531"
     misc_feature    4191..5192
                     /label=I3R
                     /note="label=I5R - 3536..4385"
     misc_feature    complement(5193..5200)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(5209..6979)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgccaccg aatattaaat aatctttttt tatttcttat gtacgcctgc
       61 tttgtatgat acaaaatcat cgtatcgtat tcggcttcat gttatccgtc gaatttagat
      121 taccttggct aaggggccaa ggtggatgat tcgtatgagt atcacaattt acaaattaca
      181 agttttgcaa agagtatgta aactgtaaaa aagaaaacat tgacaaatat acctgaaaag
      241 agtccatttt ctacatttga gaaaaaaagg aataaagtga ttctcctcat cgctcataaa
      301 aaaatactat tcatgagcaa aattgagaaa aatggcaatc acattcaata ctagttagat
      361 agccatatct aaactgctgt acgtggttta atactagcac caacatcatt tgaataatgg
      421 cgaagaaacc aatttcgaaa cattcccgtg cggcaagaag atcagaagca gccgaaccgg
      481 aggctaaggc tttagagact ttacccagag ctgaaaagac agattttgca gcaacattaa
      541 tcagaactgc taacaaaaac gaagctttgc tcgaagcgaa gatccaaaag cgtggacaaa
      601 aaaataagaa gaaaaacgcg ggaagagtga gtaagcaact tatagatgac agttctttaa
      661 caagtaggaa agctattcgt gccttaaata ttaccagtag gctcgatggt aagagggaga
      721 aagccattca tagagctaaa tatgtgcagt cagctcgtaa agctggatgg gatactacaa
      781 accaaagcat taggaaagaa ttatcgacac tacaaaacgc agcaaatacg gtcagcgatt
      841 ccgttaacaa aagcacggac aaggaagggt ctgcgaatac tgaagatgcg atgatagaag
      901 acgatcaaga tcacattgaa atttacgaca gtgctaaaag ccagaaggta caagaaaaag
      961 cagtatcaac cacttttacg gcatcaaacg tatttacgtc attagaggat gagatgaatt
     1021 cgcatctaga ctgatgagac gaacgtgaga ccgaaagtga aacgtgattt catgcgtcat
     1081 tttgaacatt ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttatga
     1141 atgttttctt aacatcgcgg caactcaaga aacggcaggt tcggatctta gctactagag
     1201 aaagaggaga aatactagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
     1261 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
     1321 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
     1381 gttccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
     1441 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
     1501 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
     1561 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaggacggc
     1621 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
     1681 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
     1741 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
     1801 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
     1861 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
     1921 gaactgtaca aatgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1981 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2041 cgggtgggcc tttctgcgtt tataggtctc agctggaaat ctgctcgtca gtggtgctca
     2101 cactgacgaa tcatgtacag atcataccga tgactgcctg gcgactcaca actaagcaag
     2161 acagccggaa ccagcgccgg cgaacaccac tgcatatatg gcatatcaca acagtccacg
     2221 tctcaagcag ttacagagat gttacgaacc actagtgcac tgcagtacat cgaggagaac
     2281 ttctagtata tctacatacc taatattatt gccttattaa aaatggaatc ccaacaatta
     2341 catcaaaatc cacattctct tcaaaatcaa ttgtcctgta cttccttgtt catgtgtgtt
     2401 caaaaacgtt atatttatag gataattata ctctatttct caacaagtaa ttggttgttt
     2461 ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg accgcagtta actgtgggaa
     2521 tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat ttttttcctc
     2581 aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg actggaaatt
     2641 ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa aattgggaga
     2701 aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa gcgttcatga
     2761 ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc tattgttttt
     2821 tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt acatttcagc
     2881 aatatatata tatatatttc aaggatatac cattctaatg tctgccccta agaagatcgt
     2941 cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta aggttcttaa
     3001 agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt taattggtgg
     3061 tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag cctccaagaa
     3121 ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggaaccg gtagtgttag
     3181 acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca acttaagacc
     3241 atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac aatttgctaa
     3301 aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg gtaagagaaa
     3361 ggaggacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc cagaagtgca
     3421 aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat tgcctatttg
     3481 gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa ctgtggagga
     3541 aaccatcaag aacgaatttc ctacattgaa ggttcaacat caattgattg attctgccgc
     3601 catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca gcaacatgtt
     3661 tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt tgttgccatc
     3721 tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg aaccatgcca
     3781 cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta tcttgtctgc
     3841 tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca ttgaagatgc
     3901 agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt ccaacagtac
     3961 caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aacctggagg
     4021 acccttctct ttagactatt ctactcttat gcacgtaaaa aattctagga aatatgtatt
     4081 aactaggagt aaaataaccg gctagtggca ttcatatagc cgtctgttta catctacatc
     4141 acacatttcg agtgtatatc tcgcaacgtt ggcgttaaat aggcaggagt tgaagcatag
     4201 aagaaatgtt taaccaaaaa ctatcctttt tatttatttt gaattttctc tctcatattt
     4261 ttgtaaaacg atactttaac cacactcaag cttttgcttg ctactcaaat acctgaaatc
     4321 tttatagaca attttccttc ttattcgtaa taataaaact atattgttct cttcctgttt
     4381 ttttttttaa aaaaaaaaac tgtcaaaatc tcccccacag aaaaataaaa acaagttata
     4441 cttttgggag taaaccatga acttaaaaca acagttcata ttcttaagta gtgccactat
     4501 agttaaccag agacaattta actcctaaat cttcatactg aagattgaca taaactaaat
     4561 aatgactaca ctgttacttt tgagtcagtt gggtcttttg gcattcgcca atgctattaa
     4621 ccctatggta aagggcgaat atagaattga acatcctaag ataggagact tgtcagaaat
     4681 tgaatctgct atctctccca aatctttcta tgaatcattt caatatggtt ccgaagcgga
     4741 gttccgtaag aattgggttt cttctgatct aacaatggaa catgtagacg aaacatcttc
     4801 gaaggcgttc cctggcacat ggaagttgca gtcaccattt ttggtcccag gattcagtga
     4861 tgatctatct ttagttttgg gtagcgaaca aactagagct gctattgccc ataggttaaa
     4921 atacgatcta tcgatcaagg ataatgagaa actagtggtg caatatgaag ttaaacttca
     4981 aaaaatgctt gaatgtggcg gtgcatatat caaattgttg aaaacctccg caactgatct
     5041 ttctcagtac gaccactcgt cagatgacta tgctgtcatc tttggcccag acttttgcca
     5101 aggttatgtg gacgaaattc atttggtttt gaagcgcgag aatccttttt ctggaaaatc
     5161 tgaagacaaa ttcttattaa atgccccaaa acggcgcgcc tcagccgagc ggccgcgtgt
     5221 tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat
     5281 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     5341 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     5401 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     5461 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
     5521 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     5581 aaaccgttat tcattcgtga ttgcgcctga gcgaggcgaa atacgcgatc gctgttaaaa
     5641 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     5701 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
     5761 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     5821 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     5881 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     5941 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     6001 tccatgttgg aatttaatcg cggcctggag caagacgttt cccgttgaat atggctcata
     6061 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
     6121 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat
     6181 cgaacttttg ctgagttgaa ggatcagtca tgaccaaaat cccttaacgt gagttttcgt
     6241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6901 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6961 tggataaccg tgcggccgc
//
