LOCUS       Exported                6676 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..1015
                     /label=I3L
     misc_feature    1016..1041
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             1046..2071
                     /codon_start=1
                     /label=GFP dropout
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    2076..2265
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2270..3883
                     /label=hphMX
     misc_feature    3888..4889
                     /label=I3R
                     /note="label=I5R - 3536..4385"
     misc_feature    complement(4890..4897)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4906..6676)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgccaccg aatattaaat aatctttttt tatttcttat gtacgcctgc
       61 tttgtatgat acaaaatcat cgtatcgtat tcggcttcat gttatccgtc gaatttagat
      121 taccttggct aaggggccaa ggtggatgat tcgtatgagt atcacaattt acaaattaca
      181 agttttgcaa agagtatgta aactgtaaaa aagaaaacat tgacaaatat acctgaaaag
      241 agtccatttt ctacatttga gaaaaaaagg aataaagtga ttctcctcat cgctcataaa
      301 aaaatactat tcatgagcaa aattgagaaa aatggcaatc acattcaata ctagttagat
      361 agccatatct aaactgctgt acgtggttta atactagcac caacatcatt tgaataatgg
      421 cgaagaaacc aatttcgaaa cattcccgtg cggcaagaag atcagaagca gccgaaccgg
      481 aggctaaggc tttagagact ttacccagag ctgaaaagac agattttgca gcaacattaa
      541 tcagaactgc taacaaaaac gaagctttgc tcgaagcgaa gatccaaaag cgtggacaaa
      601 aaaataagaa gaaaaacgcg ggaagagtga gtaagcaact tatagatgac agttctttaa
      661 caagtaggaa agctattcgt gccttaaata ttaccagtag gctcgatggt aagagggaga
      721 aagccattca tagagctaaa tatgtgcagt cagctcgtaa agctggatgg gatactacaa
      781 accaaagcat taggaaagaa ttatcgacac tacaaaacgc agcaaatacg gtcagcgatt
      841 ccgttaacaa aagcacggac aaggaagggt ctgcgaatac tgaagatgcg atgatagaag
      901 acgatcaaga tcacattgaa atttacgaca gtgctaaaag ccagaaggta caagaaaaag
      961 cagtatcaac cacttttacg gcatcaaacg tatttacgtc attagaggat gagatgaatt
     1021 cgcatctaga ctgatgagac gaacgtgaga ccgaaagtga aacgtgattt catgcgtcat
     1081 tttgaacatt ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttatga
     1141 atgttttctt aacatcgcgg caactcaaga aacggcaggt tcggatctta gctactagag
     1201 aaagaggaga aatactagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
     1261 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
     1321 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
     1381 gttccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
     1441 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
     1501 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
     1561 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaggacggc
     1621 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
     1681 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
     1741 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
     1801 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
     1861 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
     1921 gaactgtaca aatgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1981 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2041 cgggtgggcc tttctgcgtt tataggtctc agctggaaat ctgctcgtca gtggtgctca
     2101 cactgacgaa tcatgtacag atcataccga tgactgcctg gcgactcaca actaagcaag
     2161 acagccggaa ccagcgccgg cgaacaccac tgcatatatg gcatatcaca acagtccacg
     2221 tctcaagcag ttacagagat gttacgaacc actagtgcac tgcagtacag tttagcttgc
     2281 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
     2341 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
     2401 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
     2461 ttacggctcc tcgctccaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
     2521 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
     2581 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
     2641 acataaacaa aaatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     2701 atcgaaaagt tcgacagcgt gtccgacctg atgcagctct cggagggcga agaatctcgt
     2761 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     2821 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     2881 gaagtgcttg acattgggga atttagcgag agcctgacct attgcatctc ccgccgtgca
     2941 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcaaccggtc
     3001 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     3061 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     3121 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     3181 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     3241 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     3301 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     3361 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     3421 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     3481 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     3541 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     3601 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     3661 cgtccgaggg caaaggaata aagtaactga caataaaaag attcttgttt tcaagaactt
     3721 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     3781 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt
     3841 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctggagttga agcatagaag
     3901 aaatgtttaa ccaaaaacta tcctttttat ttattttgaa ttttctctct catatttttg
     3961 taaaacgata ctttaaccac actcaagctt ttgcttgcta ctcaaatacc tgaaatcttt
     4021 atagacaatt ttccttctta ttcgtaataa taaaactata ttgttctctt cctgtttttt
     4081 tttttaaaaa aaaaaactgt caaaatctcc cccacagaaa aataaaaaca agttatactt
     4141 ttgggagtaa accatgaact taaaacaaca gttcatattc ttaagtagtg ccactatagt
     4201 taaccagaga caatttaact cctaaatctt catactgaag attgacataa actaaataat
     4261 gactacactg ttacttttga gtcagttggg tcttttggca ttcgccaatg ctattaaccc
     4321 tatggtaaag ggcgaatata gaattgaaca tcctaagata ggagacttgt cagaaattga
     4381 atctgctatc tctcccaaat ctttctatga atcatttcaa tatggttccg aagcggagtt
     4441 ccgtaagaat tgggtttctt ctgatctaac aatggaacat gtagacgaaa catcttcgaa
     4501 ggcgttccct ggcacatgga agttgcagtc accatttttg gtcccaggat tcagtgatga
     4561 tctatcttta gttttgggta gcgaacaaac tagagctgct attgcccata ggttaaaata
     4621 cgatctatcg atcaaggata atgagaaact agtggtgcaa tatgaagtta aacttcaaaa
     4681 aatgcttgaa tgtggcggtg catatatcaa attgttgaaa acctccgcaa ctgatctttc
     4741 tcagtacgac cactcgtcag atgactatgc tgtcatcttt ggcccagact tttgccaagg
     4801 ttatgtggac gaaattcatt tggttttgaa gcgcgagaat cctttttctg gaaaatctga
     4861 agacaaattc ttattaaatg ccccaaaacg gcgcgcctca gccgagcggc cgcgtgttac
     4921 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     4981 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     5041 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     5101 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     5161 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
     5221 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     5281 ccgttattca ttcgtgattg cgcctgagcg aggcgaaata cgcgatcgct gttaaaagga
     5341 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     5401 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
     5461 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     5521 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     5581 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     5641 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     5701 atgttggaat ttaatcgcgg cctggagcaa gacgtttccc gttgaatatg gctcataaca
     5761 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     5821 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     5881 acttttgctg agttgaagga tcagtcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6121 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6661 ataaccgtgc ggccgc
//
