LOCUS       Exported                5854 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..864
                     /label=I4L
     misc_feature    865..890
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             895..1920
                     /codon_start=1
                     /label=GFP dropout
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1925..2114
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2119..3228
                     /label=ScHIS3
     misc_feature    3233..4067
                     /label=I4R
     misc_feature    complement(4068..4075)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4084..5854)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgcctcat accgtttatg attgcattta cgttactgac cggagttttg
       61 tccgtatttg tgaagccgtt ggagataatt gaggcaccaa ggttcgaacc agactatgct
      121 atattctacc caggaattaa ttgccagtca tgccatgctg ttaaagtacc ccgttcgaaa
      181 cactgttcac tgtgtcggcg atgcattcca ttgcaagatc atcactgcat ttggatcaac
      241 aattgtgttg gctacggtaa ctatgaatac ttctatccct tcctactagc gaactgtacc
      301 attttatcgt atgcatccat cagactattt tcaatgttga gagttctgcg agttgtgagc
      361 aaacatttcc tgtctctctt tttgttaaca acagcgtttg cattacttgc cataatattt
      421 acatactacc agttaaagct cgttcaagaa ggaatgacca acaacgagca agataaatgg
      481 tacctcgtac atgaacatat gagggatgga aaccttttca aggataacaa tggctcactg
      541 tactacagaa ccagttctgg tgtggaagga attgatgaca cattttactc taccaacttg
      601 tacgatcaca gaaagtacaa actcaacaat ccttcgagaa tacacagcgc ggaagaactc
      661 acaaatattt atgatagagg agacttctgg aagaaccttg tagagaggat aagtgtcctt
      721 tgaaaatctg actttaagaa ttctcgtagc tccatgtaca taataaatat agactaataa
      781 gattcgcata acaaactggc ttcaaatcat aatggcaaag aatcacaaaa ttcgagattt
      841 caatctagct aatattccta accggaattc gcatctagac tgatgagacg aacgtgagac
      901 cgaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat
      961 gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa
     1021 acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg cgtaaaggcg
     1081 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
     1141 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
     1201 agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga
     1261 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
     1321 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
     1381 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
     1441 tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg gaatacaatt
     1501 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
     1561 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
     1621 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
     1681 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
     1741 ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc atcaaataaa
     1801 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1861 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggtctca
     1921 gctggaaatc tgctcgtcag tggtgctcac actgacgaat catgtacaga tcataccgat
     1981 gactgcctgg cgactcacaa ctaagcaaga cagccggaac cagcgccggc gaacaccact
     2041 gcatatatgg catatcacaa cagtccacgt ctcaagcagt tacagagatg ttacgaacca
     2101 ctagtgcact gcagtacaaa cacagtcctt tcccgcaatt ttctttttct attactcttg
     2161 gcctcctcta gtacactcta tattttttta tgcctcggta atgattttca tttttttttt
     2221 tccacctagc ggatgactct ttttttttct tagcgattgg cattatcaca taatgaatta
     2281 tacattatat aaagtaatgt gatttcttcg aagaatatac taaaaaatga gcaggcaaga
     2341 taaacgaagg caaagatgac agagcagaaa gccctagtaa agcgtattac aaatgaaacc
     2401 aagattcaga ttgcgatctc tttaaagggt ggtcccctag cgatagagca ctcgatcttc
     2461 ccagaaaaag aggcagaagc agtagcagaa caggccacac aatcgcaagt gattaacgtc
     2521 cacacaggta tagggtttct ggaccatatg atacatgctc tggccaagca ttccggctgg
     2581 tcgctaatcg ttgagtgcat tggtgactta cacatagacg accatcacac cactgaggac
     2641 tgcgggattg ctctcggtca agcttttaaa gaggccctag gggccgtgcg tggagtaaaa
     2701 aggtttggat caggatttgc gcctttggat gaggcacttt ccagagcggt ggttgatctt
     2761 tcgaacaggc cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggtgatctc
     2821 tcttgcgaga tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc
     2881 cacgttgatt gtctgcgagg caagaatgat catcaccgta gtgagagtgc gttcaaggct
     2941 cttgcggttg ccataagaga agccacctcg cccaatggta ccaacgatgt tccctccacc
     3001 aaaggtgttc ttatgtagtg acaccgatta tttaaagctg ctgcatacga tatatataca
     3061 tgtgtatata tgtataccta tgaatgtcag taagtatgta tacgaacagt atgatactga
     3121 agatgacaag gtaatgcatc attctatacg tgtcattctg aacgaggcgc gctttccttt
     3181 tttctttttg ctttttcttt ttttttctct tgaactcgac ggatcataga gtaattcaag
     3241 gttgttcaat attccgattg ttagtagatt cagttctacc ttcgctcagt tttcttaatg
     3301 actcaaagga tattagaatt gaaaaatttc aaaaaagctt taagcataga accacgcact
     3361 tattaaatac aatggagaaa tgatttggtg ggcaattacc ggtttcaatt gactccgaac
     3421 aggttcaaat acaccacatt taacagacta ctaagaaaga gtcaaaaaga tttggctgaa
     3481 cattaaggga ggctaggttt tttagtgact ttttgcgaga gaaacgcttt tttagcagat
     3541 taagcaataa gtttaaacat gtccgttgaa ttgtccaaag tatccatctt gggtcaggac
     3601 accattcatg tcggatacca gattcaggaa cacattgtga agactactct ggaacaatgc
     3661 aaatcgtcga catatgttat catcaacgat actaatgtct accaggtgcc ttactaccat
     3721 gacttggttg agctatttga gaagtctcta cccgagactt cccgtttgct cacatatgtt
     3781 gtgaagccag gcgaggctaa caagacccgt gaaactaagg cacagattga agactttatg
     3841 ttgaatgaag ggtgcactcg tgataccgtc gttattgcaa ttggtggtgg tgtgatcggt
     3901 gatatgattg ggtttgttgc tgcgacattc atgagaggtg ttcgtttcat tcaaattcct
     3961 acatcgctat tggcaatggt tgactcgtct attggtggta agactgccgt tgacacccca
     4021 atcggtaaaa acttcattgg tgccttctgg caaccacaat ttgtgttggc gcgcctcagc
     4081 cgagcggccg cgtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
     4141 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     4201 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     4261 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     4321 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     4381 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     4441 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag gcgaaatacg
     4501 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     4561 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     4621 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     4681 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     4741 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     4801 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     4861 ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt
     4921 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     4981 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     5041 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agtcatgacc aaaatccctt
     5101 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5161 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5221 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5281 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5341 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5401 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5461 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5521 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5581 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5641 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5701 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5761 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5821 tatcccctga ttctgtggat aaccgtgcgg ccgc
//
