LOCUS       Exported                6135 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..864
                     /label=I4L
     misc_feature    865..890
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             895..1920
                     /codon_start=1
                     /label=GFP dropout
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1925..2114
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2119..3509
                     /label=kanMX
                     /note="label=kanMX - 2141..3531"
     misc_feature    3514..4348
                     /label=I4R
     misc_feature    complement(4349..4356)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4365..6135)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgcctcat accgtttatg attgcattta cgttactgac cggagttttg
       61 tccgtatttg tgaagccgtt ggagataatt gaggcaccaa ggttcgaacc agactatgct
      121 atattctacc caggaattaa ttgccagtca tgccatgctg ttaaagtacc ccgttcgaaa
      181 cactgttcac tgtgtcggcg atgcattcca ttgcaagatc atcactgcat ttggatcaac
      241 aattgtgttg gctacggtaa ctatgaatac ttctatccct tcctactagc gaactgtacc
      301 attttatcgt atgcatccat cagactattt tcaatgttga gagttctgcg agttgtgagc
      361 aaacatttcc tgtctctctt tttgttaaca acagcgtttg cattacttgc cataatattt
      421 acatactacc agttaaagct cgttcaagaa ggaatgacca acaacgagca agataaatgg
      481 tacctcgtac atgaacatat gagggatgga aaccttttca aggataacaa tggctcactg
      541 tactacagaa ccagttctgg tgtggaagga attgatgaca cattttactc taccaacttg
      601 tacgatcaca gaaagtacaa actcaacaat ccttcgagaa tacacagcgc ggaagaactc
      661 acaaatattt atgatagagg agacttctgg aagaaccttg tagagaggat aagtgtcctt
      721 tgaaaatctg actttaagaa ttctcgtagc tccatgtaca taataaatat agactaataa
      781 gattcgcata acaaactggc ttcaaatcat aatggcaaag aatcacaaaa ttcgagattt
      841 caatctagct aatattccta accggaattc gcatctagac tgatgagacg aacgtgagac
      901 cgaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat
      961 gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa
     1021 acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg cgtaaaggcg
     1081 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
     1141 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
     1201 agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga
     1261 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
     1321 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
     1381 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
     1441 tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg gaatacaatt
     1501 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
     1561 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
     1621 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
     1681 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
     1741 ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc atcaaataaa
     1801 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1861 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggtctca
     1921 gctggaaatc tgctcgtcag tggtgctcac actgacgaat catgtacaga tcataccgat
     1981 gactgcctgg cgactcacaa ctaagcaaga cagccggaac cagcgccggc gaacaccact
     2041 gcatatatgg catatcacaa cagtccacgt ctcaagcagt tacagagatg ttacgaacca
     2101 ctagtgcact gcagtacaag cttgcctcgt ccccgccggg tcacccggcc agcgacatgg
     2161 aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca tgatgtgact
     2221 gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc atacattttg
     2281 atggccgcac ggcgcgaagc aaaaattacg gctcctcgct ccagacctgc gagcagggaa
     2341 acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag agaaatataa
     2401 aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa atcttgctag
     2461 gatacagttc tcacatcaca tccgaacata aacaaaaatg ggtaaggaaa agactcacgt
     2521 ttcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     2581 cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc
     2641 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     2701 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     2761 tcctgatgat gcatggttac tcaccactgc gatccccggc aaaacagcat tccaggtatt
     2821 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     2881 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctggc
     2941 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     3001 taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc
     3061 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     3121 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     3181 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     3241 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     3301 tttctaaagt aactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt
     3361 ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc
     3421 gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca
     3481 atcgtatgtg aatgctggtc gctatactgg agtaattcaa ggttgttcaa tattccgatt
     3541 gttagtagat tcagttctac cttcgctcag ttttcttaat gactcaaagg atattagaat
     3601 tgaaaaattt caaaaaagct ttaagcatag aaccacgcac ttattaaata caatggagaa
     3661 atgatttggt gggcaattac cggtttcaat tgactccgaa caggttcaaa tacaccacat
     3721 ttaacagact actaagaaag agtcaaaaag atttggctga acattaaggg aggctaggtt
     3781 ttttagtgac tttttgcgag agaaacgctt ttttagcaga ttaagcaata agtttaaaca
     3841 tgtccgttga attgtccaaa gtatccatct tgggtcagga caccattcat gtcggatacc
     3901 agattcagga acacattgtg aagactactc tggaacaatg caaatcgtcg acatatgtta
     3961 tcatcaacga tactaatgtc taccaggtgc cttactacca tgacttggtt gagctatttg
     4021 agaagtctct acccgagact tcccgtttgc tcacatatgt tgtgaagcca ggcgaggcta
     4081 acaagacccg tgaaactaag gcacagattg aagactttat gttgaatgaa gggtgcactc
     4141 gtgataccgt cgttattgca attggtggtg gtgtgatcgg tgatatgatt gggtttgttg
     4201 ctgcgacatt catgagaggt gttcgtttca ttcaaattcc tacatcgcta ttggcaatgg
     4261 ttgactcgtc tattggtggt aagactgccg ttgacacccc aatcggtaaa aacttcattg
     4321 gtgccttctg gcaaccacaa tttgtgttgg cgcgcctcag ccgagcggcc gcgtgttaca
     4381 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     4441 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     4501 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     4561 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     4621 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc
     4681 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     4741 cgttattcat tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac
     4801 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     4861 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     4921 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     4981 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     5041 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     5101 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     5161 tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg ctcataacac
     5221 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     5281 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
     5341 cttttgctga gttgaaggat cagtcatgac caaaatccct taacgtgagt tttcgttcca
     5401 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5461 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5521 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5581 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5641 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5701 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5761 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5821 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5881 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5941 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6001 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6061 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6121 taaccgtgcg gccgc
//
