LOCUS       Exported                5875 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..866
                     /label=I5L
     misc_feature    867..892
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             897..1922
                     /codon_start=1
                     
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1927..2116
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2121..3230
                     /label=ScHIS3
                     /note="label=kanMX - 2141..3531"
     misc_feature    3234..4084
                     /label=I5R
                     /note="label=I5R - 3536..4385"
     misc_feature    complement(4085..4092)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4096..5875)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgccggtg ttgcattagt atcgccgtgg atgaagatga aggtggaata
       61 gacgttaccc atagataccc gaatgagtat ctgattgagg atttttgact tacttacttc
      121 ttcgagatac actactctgc cgaaatttcg gactggaatg cgtcaaagtg aatccacttt
      181 gcaggaaaac gcaggaaaaa cggattcgaa aacgcaacaa aaaaaaaaaa attatacata
      241 ataaaatttg aatcaaagca acaagttcac gaatccatta aagcagtcag aatccaggaa
      301 aaaaaagaaa cagagccaga aaaaaggcag caacgaatca caaccacagc gtattgcgtc
      361 agtggaaatt aattaccttg gtatttccaa tgctgtgtac aaccaaaatt tccacatagt
      421 tagttgattc gcatttccct ggtaaccgcc ctcggcaacc tccctcggaa acttccctcc
      481 agagtaactt cccccagagt tatagagata tagagttata gtagagatat agagctatag
      541 atagattgat tcttgttgta gcatttattc cgttaaactt ctgcggctaa ttttgcctag
      601 cttggccttt tttttttgtt tttcgagctt tcaaagacaa atggacattc ttacataata
      661 tattatcata tacaccgaga tcggtgggtc agagcctgta gggatggaca ttttccttct
      721 agactcgcct tccagacctt tttttggttt gtttctctct ccgtggaact cgctttcttt
      781 ttcgccggtg atctcgaaac gtaatcgata tgtattccat tccattcgct attttttttg
      841 ttccataaac catgttctta tgaacagaat tcgcatctag actgatgaga cgaacgtgag
      901 accgaaagtg aaacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
      961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     1021 aaacggcagg ttcggatctt agctactaga gaaagaggag aaatactaga tgcgtaaagg
     1081 cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg
     1141 tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct
     1201 gaagttcatc tgtactactg gtaaactgcc ggttccttgg ccgactctgg taacgacgct
     1261 gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt
     1321 caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg
     1381 cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga
     1441 gctgaaaggc attgacttta aagaggacgg caatatcctg ggccataagc tggaatacaa
     1501 ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa
     1561 ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca
     1621 aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca
     1681 aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt
     1741 aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgaccag gcatcaaata
     1801 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1861 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttataggtct
     1921 cagctggaaa tctgctcgtc agtggtgctc acactgacga atcatgtaca gatcataccg
     1981 atgactgcct ggcgactcac aactaagcaa gacagccgga accagcgccg gcgaacacca
     2041 ctgcatatat ggcatatcac aacagtccac gtctcaagca gttacagaga tgttacgaac
     2101 cactagtgca ctgcagtaca aacacagtcc tttcccgcaa ttttcttttt ctattactct
     2161 tggcctcctc tagtacactc tatatttttt tatgcctcgg taatgatttt catttttttt
     2221 tttccaccta gcggatgact cttttttttt cttagcgatt ggcattatca cataatgaat
     2281 tatacattat ataaagtaat gtgatttctt cgaagaatat actaaaaaat gagcaggcaa
     2341 gataaacgaa ggcaaagatg acagagcaga aagccctagt aaagcgtatt acaaatgaaa
     2401 ccaagattca gattgcgatc tctttaaagg gtggtcccct agcgatagag cactcgatct
     2461 tcccagaaaa agaggcagaa gcagtagcag aacaggccac acaatcgcaa gtgattaacg
     2521 tccacacagg tatagggttt ctggaccata tgatacatgc tctggccaag cattccggct
     2581 ggtcgctaat cgttgagtgc attggtgact tacacataga cgaccatcac accactgagg
     2641 actgcgggat tgctctcggt caagctttta aagaggccct aggggccgtg cgtggagtaa
     2701 aaaggtttgg atcaggattt gcgcctttgg atgaggcact ttccagagcg gtggttgatc
     2761 tttcgaacag gccgtacgca gttgtcgaac ttggtttgca aagggagaaa gtaggtgatc
     2821 tctcttgcga gatgatcccg cattttcttg aaagctttgc agaggctagc agaattaccc
     2881 tccacgttga ttgtctgcga ggcaagaatg atcatcaccg tagtgagagt gcgttcaagg
     2941 ctcttgcggt tgccataaga gaagccacct cgcccaatgg taccaacgat gttccctcca
     3001 ccaaaggtgt tcttatgtag tgacaccgat tatttaaagc tgctgcatac gatatatata
     3061 catgtgtata tatgtatacc tatgaatgtc agtaagtatg tatacgaaca gtatgatact
     3121 gaagatgaca aggtaatgca tcattctata cgtgtcattc tgaacgaggc gcgctttcct
     3181 tttttctttt tgctttttct ttttttttct cttgaactcg acggatcata gagtttcaca
     3241 atggagggag tgaaatctaa aagtggaaaa aaaaagggga ggcgaaaaac aaaaaaaaaa
     3301 aaaagaacga accgaactat gtccacagga gcagaagcag aagctgcggc agcagcttct
     3361 gcggctttga aagaggaaac gcgtagatga aagaatgatc caagcccggc tttccctctg
     3421 tgcgcggatt gttatttttt tagacttatt tctatgtttg ttttggacct aatataactt
     3481 agtcatgaat cctggttgac tcgcgcgcat ctttttccac tctgcgatga ttcacagtgc
     3541 agcgtgcagc tcagctccag cagcagtacc agcggcagct ccaacagtgg agccttcctt
     3601 tcacgaacgg aatagaaaca ggaaggaaaa aaatagacca acaagaggac ccatccgcga
     3661 cttctaattt ttggcgcatg aaccgcgctg ttctacccgc ccgtccgtcc tcatccctcc
     3721 cccccaggaa aacaggaaat tgggaaacct aggtgcgtgc attttcgttt tgggcgatga
     3781 ttgacaatga gacttagtcc gcttggttgg gtttcgtttc aaaaacaagg tttggaagaa
     3841 gatcttggga gagaaagacc gagagatctt gaccagggcg aagacgacca gtagcttttg
     3901 gtatagccgg gataagcttc aggcacccta gggttgtata agagttgtat gcaaggatgt
     3961 gcgcaagggt ttctgaagca ggatggaaat gtggtgtgtt tttttgtaga gcaattgcct
     4021 taaagtcggg gattacgccg agacagcagc ttgacggttc cgtggtaata tgtgtccggc
     4081 aacaggcgcg cctcagccga ccgagcggcc gcgtgttaca accaattaac caattctgat
     4141 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     4201 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     4261 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     4321 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     4381 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     4441 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     4501 gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     4561 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     4621 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     4681 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     4741 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     4801 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     4861 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     4921 ctggagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     4981 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     5041 agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat
     5101 cagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5161 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5221 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5281 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5341 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5401 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5461 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5521 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5581 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5641 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5701 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5761 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5821 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg gccgc
//
