LOCUS       Exported                5844 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    29..36
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    37..889
                     /label=I6L
                     /note="Left homology arm flanking the ARO3 gene in 
                     K.marxianus"
     misc_feature    890..915
                     /label=ConLS1' overhang
     CDS             920..1945
                     /codon_start=1
                     /label=GFP dropout
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1950..2139
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2144..3202
                     /label=ScURA3
                     /note="label=kanMX - 2141..3531"
     misc_feature    3207..4053
                     /label=I6R
                     /note="Right homology arm flanking the ARO3 gene in 
                     K.marxianus"
     misc_feature    complement(4054..4061)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4074..5844)
                     /label=KanR/ColE1
                     /note="label=KanR/ColE1 - complement(4406..6176)"
ORIGIN
        1 gcatcgtctc atcggtctca ccctttcagg cgcgcctaag gcagtagagc agtagcaaag
       61 agcaaaacca aaggaaaaaa aaaataaaaa taaaaaaagg gaagggaaac cgacccaccg
      121 accttcaatt cgctgacata atgctcatat attctacaac cactctacgt aataaattcc
      181 acgatgtact ttatactatt tcccctgcga caagggcgcc aacgctgcta ttaccaccac
      241 tatacagaat ataatataat atagtatagt acaatatatg gtgcatatga cccccatgtg
      301 gcttatataa tcaaaatgaa aatggtgtca cgtgactcta attacttctt ggcgtgtttt
      361 cggagtagat aaacaggggc gataaacagg ggcatatacg ggggccagcg agcgagtagt
      421 gccacggccc ctttaaacac tggggtatgc cttcaagaac caaaaaaaaa attgccaatt
      481 cctaatatcg taatattcaa gaatacaagc atattcaaat tttgcggagt tataactcag
      541 ctggcaccaa ttacatccac gtgacccgat ccagcgccgg attaactttc cctagaaaaa
      601 attttttttt ttttattttt tttccaattt ttccattgtt tctgccaaat tactcataat
      661 gccacatttt agaaattttt caggttcggt gagatcaagc cggttacgca caaaaagctt
      721 gaaaaattca ggaaccgtgg tatataatga atcatcgcat gatgagagag agagaagaaa
      781 gagaagaata aataataacc attaagagtt gttgttgttt cattatttca gttgtattcc
      841 ttttgtattt ttttcttgtt agataggtaa ctaacggtaa taagtgtagg aattcgcatc
      901 tagactgatg agacgaacgt gagaccgaaa gtgaaacgtg atttcatgcg tcattttgaa
      961 cattttgtaa atcttattta ataatgtgtg cggcaattca catttaattt atgaatgttt
     1021 tcttaacatc gcggcaactc aagaaacggc aggttcggat cttagctact agagaaagag
     1081 gagaaatact agatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc tattctggtg
     1141 gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac
     1201 gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact gccggttcct
     1261 tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg ttatccggac
     1321 catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc
     1381 acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc
     1441 gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagagga cggcaatatc
     1501 ctgggccata agctggaata caattttaac agccacaatg tttacatcac cgccgataaa
     1561 caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga tggcagcgtg
     1621 cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt tctgctgcca
     1681 gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat
     1741 catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat ggatgaactg
     1801 tacaaatgac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1861 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1921 ggcctttctg cgtttatagg tctcagctgg aaatctgctc gtcagtggtg ctcacactga
     1981 cgaatcatgt acagatcata ccgatgactg cctggcgact cacaactaag caagacagcc
     2041 ggaaccagcg ccggcgaaca ccactgcata tatggcatat cacaacagtc cacgtctcaa
     2101 gcagttacag agatgttacg aaccactagt gcactgcagt acacggtttc cttgaaattt
     2161 ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt
     2221 ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
     2281 actgcacaga acaaaaacgt gcaggaaacg aagataaatc atgtcgaaag ctacatataa
     2341 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
     2401 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
     2461 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga
     2521 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
     2581 actcttcgag gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
     2641 gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
     2701 aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct
     2761 tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg
     2821 tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga
     2881 catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga
     2941 tgacaaggga gatgcattgg gtcaacagta tagaaccgtg gatgatgtgg tttctacagg
     3001 atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga
     3061 gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa
     3121 ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat
     3181 ttaattatat cagttattac ccgagtaggc taaggttgtt gatgctgaag gtgttcaaag
     3241 aagaattatg attgattgct ctcatggtaa ctccagcaaa gacttcagaa atcagccaaa
     3301 ggttgcccaa tccatctacg aacaacttac cgatggacaa aatgctatct gcggtgtaat
     3361 gatcgagtct aacttggtag aaggcagaca agatattcct ccagaaggtg gccgtgacaa
     3421 attgatctac ggatgttcaa ttactgatgc ttgtattggt tgggatacca ctgatgaggt
     3481 acttcgtcta ttggccaaag gtgtccaaaa tagaagagca gcattgaaga aataagtcaa
     3541 ctaaaactct tttataaaac aaaacaaaca aaatgacaaa ttaatcccga ttaatttagg
     3601 cgtccttcct gtagttgctt gccaaaagac gagcgagagc ccagaataaa tcaaaaacta
     3661 tttatcctct tggtatggaa cgtatatatg tatacatata taataataat tatctatttt
     3721 ataaagatga aaagttcaat ttcatacatt ctattctagt gtgaaagtgt acatatgtat
     3781 aaaagcgaaa attgatgttg agctgatcga atgtttgtat gatagcaagt ttttcttctt
     3841 tctccttttg tttgctagaa cgatgtggca ttataagatt attgtttgca aagcggaatt
     3901 taacctattg aaagtgattc ttcactcata aaaggtaaaa aaaaaaaaag aagagagaga
     3961 gacattgaaa atgtggaatg aagactatta gtatagtaga attagaatta aaattgaaac
     4021 tcagttcatg tagaatcagt tgtaatagtg ttgggcgcgc ctcagccgac cgagcggccg
     4081 cgtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     4141 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     4201 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     4261 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     4321 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     4381 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     4441 caaccaaacc gttattcatt cgtgattgcg cctgagcgag gcgaaatacg cgatcgctgt
     4501 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     4561 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     4621 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     4681 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     4741 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     4801 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     4861 cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt tgaatatggc
     4921 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     4981 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttgttgaa
     5041 taaatcgaac ttttgctgag ttgaaggatc agtcatgacc aaaatccctt aacgtgagtt
     5101 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5161 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5221 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5281 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5341 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5401 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5461 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5521 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5581 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5641 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5701 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5761 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5821 ttctgtggat aaccgtgcgg ccgc
//
