LOCUS       pRaGE_Pyl_TAG_Pr        7890 bp    DNA     circular UNA 30-AUG-2020
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...d-ccjjqpp
VERSION     urn.local...d-ccjjqpp
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(223..1694)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="2micron origin"
                     /Transferred_Similarity="99.73%"
                     /label="2micron origin"
                     /note="Geneious type: origin of replication"
     promoter        1697..1922
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="URA3 prom"
                     /Transferred_Similarity="100.00%"
                     /label="URA3 prom"
     marker          1924..2724
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="URA3 marker"
                     /Transferred_Similarity="100.00%"
                     /label="URA3 marker"
     source          complement(<2731..>2930)
                     /organism="Vibrio natriegens NBRC 15636 = ATCC 14048 =
                     DSM759"
                     /mol_type="genomic DNA"
                     /strain="ATCC 14048"
                     /isolation_source="salt marsh mud"
                     /culture_collection="ATCC:14048"
                     /db_xref="taxon:1219067"
                     /chromosome=1
                     /country="USA"
                     /collection_date="27-Feb-2012"
                     /label="source Vibrio natriegens NBRC 15636 = ATCC 14048 =
                     DSM759"
     promoter        2731..2930
                     /created_by="User"
                     /modified_by="User"
                     /label="GluRS upstream"
     gene            2931..4295
                     /created_by="User"
                     /label="MmPylRS"
     terminator      4296..4595
                     /created_by="User"
                     /modified_by="User"
                     /label="GluRS downstream"
     source          complement(<4296..>4595)
                     /organism="Vibrio natriegens NBRC 15636 = ATCC 14048 =
                     DSM759"
                     /mol_type="genomic DNA"
                     /strain="ATCC 14048"
                     /isolation_source="salt marsh mud"
                     /culture_collection="ATCC:14048"
                     /db_xref="taxon:1219067"
                     /chromosome=1
                     /country="USA"
                     /collection_date="27-Feb-2012"
                     /label="source Vibrio natriegens NBRC 15636 = ATCC 14048 =
                     DSM759"
     source          <4596..>4677
                     /organism="Vibrio natriegens NBRC 15636 = ATCC 14048 =
                     DSM759"
                     /mol_type="genomic DNA"
                     /strain="ATCC 14048"
                     /isolation_source="salt marsh mud"
                     /culture_collection="ATCC:14048"
                     /db_xref="taxon:1219067"
                     /chromosome=1
                     /country="USA"
                     /collection_date="27-Feb-2012"
                     /label="source Vibrio natriegens NBRC 15636 = ATCC 14048 =
                     DSM759"
     promoter        4596..4677
                     /created_by="User"
                     /modified_by="User"
                     /label="tRNA upstream"
     tRNA            <4678..4749
                     /created_by="User"
                     /modified_by="User"
                     /label="Mm-PylT (tRNA)"
     tag             4708..4710
                     /created_by="User"
                     /label="Anti codon"
     terminator      4750..4872
                     /created_by="User"
                     /modified_by="User"
                     /label="tRNA downstream"
     source          <4750..>4872
                     /organism="Vibrio natriegens NBRC 15636 = ATCC 14048 =
                     DSM759"
                     /mol_type="genomic DNA"
                     /strain="ATCC 14048"
                     /isolation_source="salt marsh mud"
                     /culture_collection="ATCC:14048"
                     /db_xref="taxon:1219067"
                     /chromosome=1
                     /country="USA"
                     /collection_date="27-Feb-2012"
                     /label="source Vibrio natriegens NBRC 15636 = ATCC 14048 =
                     DSM759"
     promoter        5061..5115
                     /created_by="User"
                     /modified_by="User"
                     /label="P70b"
     gene            5133..5149
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="T7_transl_en_RBS"
                     /Transferred_Similarity="100.00%"
                     /label="T7_transl_en_RBS"
     gene            <5157..5870
                     /created_by="User"
                     /modified_by="User"
                     /label="deGFP"
     tag             5169..5186
                     /created_by="User"
                     /modified_by="User"
                     /label="6xHis"
     modified_base   5295..5297
                     /created_by="User"
                     /modified_by="User"
                     /label="Y35TAG"
     terminator      5877..5906
                     /created_by="User"
                     /label="T500"
     misc_feature    complement(6203..6822)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     marker          complement(6977..7837)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label="amp marker"
     promoter        complement(7879..17)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
ORIGIN      
        1 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
       61 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
      121 ataggcgtat cacgaggccc tttcgtcttc aagaattctg gcgaatcctc tgaccagcca
      181 gaaaacgacc tttctgtggt gaaaccggat gctgcaattc ggatccgcga ataccgcttc
      241 cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat ccctatataa
      301 cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct acatttttta
      361 tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta ttcatagagt
      421 gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag agacaaaata
      481 gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc actttctgtt
      541 cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct ttatcttgaa
      601 aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt caggcttttt
      661 ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg acctacagtg
      721 caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa gtaatctaag
      781 atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa aaagaagtat
      841 agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta aaaatgcagc
      901 tcagattctt tgtttgaaaa attagcgctc tcgtcgcgtt gcatttttgt tttacaaaaa
      961 tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa
     1021 aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac
     1081 aaaatgaagc acagatgctt cgttaacaaa gatatgctat tgaagtgcaa gatggaaacg
     1141 cagaaaatga accggggatg cgacgtgcaa gattacctat gcaatagatg caatagtttc
     1201 tccaggaacc gaaatacata cattgtcttc cgtaaagcgc tagactatat attattatac
     1261 aggttcaaat atactatctg tttcagggaa aactcccagg ttcggatgtt caaaattcaa
     1321 tgatgggtaa caagtacgat cgtaaatctg taaaacagtt tgtcggatat taggctgtat
     1381 ctcctcaaag cgtattcgaa tatcattgag aagctgcagc gtcacatcgg ataataatga
     1441 tggcagccat tgtagaagtg ccttttgcat ttctagtctc tttctcggtc tagctagttt
     1501 tactacatcg cgaagataga atcttagatc acactgcctt tgctgagctg gatcaataga
     1561 gtaacaaaag agtggtaagg cctcgttaaa ggacaaggac ctgagcggaa gtgtatcgta
     1621 cagtagacgg agtatctagt atagtctata gtccgtggaa ttaattctca tctttgacag
     1681 cttatcatcg ataagctagc ttttcaattc aattcatcat ttttttttta ttcttttttt
     1741 tgatttcggt ttctttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga
     1801 aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga agaaacatga
     1861 aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa
     1921 atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc
     1981 aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt
     2041 accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa
     2101 acacatgtgg atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca
     2161 ttatccgcca agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat
     2221 acagtcaaat tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg
     2281 aatgcacacg gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa
     2341 gtaacagagg aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta
     2401 tctactggag agtatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt
     2461 atcggcttta ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggctgatt
     2521 atgacacccg gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc
     2581 gtggatgatg tggtctctac aggatctgac attattattg ttggaagagg actatttgca
     2641 aagggaaggg atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat
     2701 ttgagaagat gcggccagca aaacggatcc gcttaaacct tactgcaagg gtaaggtcaa
     2761 ggtttgttat caatgaagtt aatttcgttg aaccccgcat cgagaggtta tttgggtata
     2821 tgaacacgga aggattgtag tattgactta cgatggtaaa atgtcgccac ttttatcctt
     2881 gacaatacaa cgagaaccct ctcgttgaac attaacccta aggaactcaa atggataaaa
     2941 aaccactaaa cactctgatc tctgctactg gtctgtggat gagtcgtacc ggaaccattc
     3001 ataaaatcaa acaccacgag gttagccgtt cgaaaatcta tattgagatg gcgtgtggcg
     3061 atcatctggt tgtgaacaat agccgctctt ctcgtacagc acgtgcactg cgtcaccaca
     3121 aatatcgtaa aacctgtaaa cgttgccgtg tgtccgatga ggatctgaac aaattcctga
     3181 caaaagccaa tgaggaccaa acaagcgtga aagtgaaagt cgttagcgct cctacccgta
     3241 ctaaaaaagc aatgccgaaa tccgttgctc gtgcccctaa accactggaa aacactgaag
     3301 cagcacaggc acagccgtct ggaagcaaat tctctccggc cattcctgtt tctacccagg
     3361 agtccgtttc tgttccagca agtgtgagca ccagcattag cagtattagc accggtgcca
     3421 ccgctagcgc cctggttaaa ggcaatacca atccgattac aagcatgtct gccccggttc
     3481 aagcatcagc tccagcactg acaaaatccc aaaccgatcg tctggaggtt ctgctgaatc
     3541 cgaaagacga aatcagcctg aattccggca aaccgtttcg tgaactggag agcgaactgc
     3601 tgtcacgtcg taaaaaagac ctgcaacaaa tctatgccga agaacgtgag aactatctgg
     3661 ggaaactgga acgtgaaatc acccgctttt tcgtggatcg tggctttctg gagatcaaat
     3721 ccccgattct gattcctctg gagtatatcg agcgtatggg catcgacaat gataccgaac
     3781 tgagcaaaca aattttccgt gtggataaaa acttctgtct gcgccctatg ctggcaccaa
     3841 atctgtataa ctatctgcgc aaactggacc gtgccctgcc tgatcctatc aaaatcttcg
     3901 agatcggccc gtgttatcgt aaagagtccg acggtaaaga acatctggag gagtttacca
     3961 tgctgaactt ttgccaaatg ggttcaggtt gtactcgtga gaacctggaa agcatcatca
     4021 ccgattttct gaaccacctg ggcattgact tcaaaattgt gggcgacagc tgtatggtgt
     4081 atggcgacac cctggatgtc atgcacggcg acctggaact gtctagtgcc gttgttggac
     4141 caattccgct ggaccgtgag tggggtatcg acaaaccgtg gatcggagca ggattcggtc
     4201 tggaacgcct gctgaaagtg aaacacgact tcaaaaacat caaacgtgcc gcccgttctg
     4261 aatcgtatta taacgggatt tctaccaacc tgtaattcaa ttgcatagat attcaaaaac
     4321 cgcggtaatt tccgcggttt ttttatgtct aaagaatgta ataaaagcga aagtagtgaa
     4381 cagatttgac tgatgtacat gctatgtcgg cgtgttgatg tttgtttttt gataaatttt
     4441 tgtaattaaa gatcgtggtt tttagtgctt attcaatggc tgattttcaa actatccctc
     4501 tgattaatat ctactatcta taaagttccc caagtggcat ctcaatagtg agacaaatat
     4561 ctgcattagt tactttgttg tgacttttgg cgcaatactc acgatgagtg tccacacaga
     4621 ttgatatggt ttatgaagtt taagagatag aacatccccc aagatgttca acttagtgga
     4681 aacctgatca tgtagatcga atggactcta aatccgttca gccgggttag attcccgggg
     4741 tttccgccat tctctaagca tttttggaat aaaacgtcca aaccagagca agcaattgac
     4801 actggttggc atttttgact ctgaaagtct ttagaaaatg tacttcctcg tgaagaaaca
     4861 tagctcttta acagagcggc agcaagtggg ggacagcaga agacctgacc gccgcagagt
     4921 ggatgtttga catggtgaag actatcgcac catcagccag aaaaccgaat tttgctgggt
     4981 gggctaacga tatccgcctg atgcgtgaac gtgacggacg taaccaccgc gacatgtgtg
     5041 tgctgttccg ctgggcatgc tgagctaaca ccgtgcgtgt agacaatttt acctctggcg
     5101 gtgataatgg ttgcagctag caataatttt gtttaacttt aagaaggaga tataccatgg
     5161 gcagcagcca tcatcatcat catcacagct ctagagagct tttcactggc gttgttccca
     5221 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     5281 agggcgatgc cacctagggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     5341 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     5401 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     5461 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     5521 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     5581 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     5641 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     5701 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5761 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     5821 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatctga ctcgagcaaa
     5881 gcccgccgaa aggcgggctt ttctgtgtcg accgatgccc ttgagagcct tcaacccagt
     5941 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     6001 tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact
     6061 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6121 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     6421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6961 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7021 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7081 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7141 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7201 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7261 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7321 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7381 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7441 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7501 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7561 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7621 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7681 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7741 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7801 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7861 gaagcattta tcagggttat tgtctcatga
//
