LOCUS       vYM082_PB-pEF1s(min)-3x 9390 bp ds-DNA     circular     27-MAR-2019
DEFINITION  .
ACCESSION   urn.local...1367881019035.10 (modified)                            
FEATURES             Location/Qualifiers
     misc_feature    5339..5801
                     /label="gYM07_iaaH"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3594..3617
                     /label="SV40-NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4404..5804
                     /label="iaaH"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(781..1440)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(1901..2207)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     complement(9003..9023)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3537..3545
                     /label="Kozak"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2559..2574)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7598..7728
                     /label="SV40 pA"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    4404..4899
                     /label="gYM05_iaaH"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(2226..2294)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5808..5820
                     /label="MCS"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     enhancer        2750..2981
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3630..4343
                     /label="Citrine"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6086..6394
                     /label="pSV40"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3239..3513
                     /label="T-Virus Homo"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3013..3506
                     /label="gYM10_pEF1s(min)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    3546..3569
                     /label="SV40-NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        3166..3172
                     /label="TATA box"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     2365..2382
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     complement(9035..9054)
                     /label="pLac(F)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     polyA_signal    5821..6035
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        8231..8237
                     /label="TATA box"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2512..2524)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7483..7587
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(1..683)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4876..5374
                     /label="gYM06_iaaH"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2525..2555)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    8482..8516
                     /label="3'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(778..1638)
                     /label="Amp"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_binding    complement(9029..9051)
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3546..3617
                     /label="Optimized 3xNLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(9055..9085)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2556..2557)
                     /label="5'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4344..4397
                     /label="T2A"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        2987..3530
                     /label="pEF1s"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2998..3228
                     /label="pEF Homo"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     enhancer        complement(7977..8207)
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3570..3593
                     /label="SV40-NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    6451..7476
                     /label="Hygro"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     2450..2469
                     /label="BII(R)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5782..5814)
                     /label="oYM438"
                     /note="sequence: CCACATCCCCGCAAGTAAGCAGTGATCCGCGTCCCTCggtGTTCGGAAGTCCAGCGAAAT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(5784..5804)
                     /label="oYM351"
                     /note="sequence: aGGTCTCgAGGTTCACAGGTCTTCCTCACTAATGAGTTTCTGCTCgccgcttccgccgccgcttccgccgccgcttccgccggtGTTCGGAAGTCCAGCGAA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4358..4389
                     /label="oYM437"
                     /note="sequence: cctcctaacttgcggagacgtcgaggagaatc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(4322..4343)
                     /label="oYM355"
                     /note="sequence: aGGTCTCgAGGTTCActtgtacagctcgtccatgccg"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(5784..5805)
                     /label="oYM350"
                     /note="sequence: aGGTCTCgTACGTCAggtGTTCGGAAGTCCAGCGAA"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3537..3555
                     /label="oYM354"
                     /note="sequence: aGGTCTCgCAGAgccaccatgcccaaaaaaa"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(5784..5804)
                     /label="oYM349"
                     /note="sequence: aGGTCTCgTACGTCACAGGTCTTCCTCACTAATGAGTTTCTGCTCgccgcttccgccgccgcttccgccgccgcttccgccggtGTTCGGAAGTCCAGCGAA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
       61 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      421 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
      481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
      961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1741 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     1801 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     1861 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     1921 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     1981 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     2041 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     2101 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     2161 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     2221 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     2281 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     2341 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt
     2401 cacgtttttg aacccgtgga ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg
     2461 atggagcaga tgaagatgct cgacacgctg cagaacacgc agctagatta accctagaaa
     2521 gataatcata ttgtgacgta cgttaaagat aatcatgtgt aaaattgacg catgtgtttt
     2581 atcggtctgt atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt
     2641 tacacttaca tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta
     2701 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttatg agggacagcc
     2761 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     2821 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     2881 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     2941 gctctttgag cctgcagaca cctgggggga tacggggaaa aggcctggat ctgccattgc
     3001 tccagtgccc ttcagtgggc agagagcaca tcacccacag tccccaagaa gttgggggga
     3061 ggggtgggca attgaactgg tgcctagaga aggtggcgca gggtaaactg ggaaagtgat
     3121 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccatatat aagtgcagta
     3181 gtccccctga acgttctttt ttgcaacggg tttgccgcca gaacacagct gaagctttga
     3241 ggggctagca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacaccgg
     3301 ttgagttgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgtgtc cgccgtctag
     3361 gtaagtttaa agctcaggtt gagaccgggc ctttgtccgg tgctcccttg gagcctacct
     3421 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
     3481 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac ctgggcctac ggatccgcca
     3541 ccatgcccaa aaaaaagcga aaagtgggtc ctaagaagaa acggaaggtt ggccccaaaa
     3601 agaaacggaa ggtcggtagt ggtggcagtg tgagcaaggg cgaggagctg ttcaccgggg
     3661 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3721 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     3781 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct
     3841 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     3901 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     3961 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4021 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     4081 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     4141 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4201 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     4261 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4321 tcggcatgga cgagctgtac aaggagggca gggggagcct cctaacttgc ggagatgtcg
     4381 aggagaatcc tggcccaGGT GGAGTAGCCA TCACGTCACT GGCCCAATCT TTGGAGCATC
     4441 TTAAACGAAA AGACTATTCT TGTCTTGAAT TGGTTGAGAC ACTTATTGCC AGATGCGAGG
     4501 CCGCGAAAAG TCTTAATGCT CTTCTCGCAA CGGATTGGGA CGGGTTGAGG AGATCCGCTA
     4561 AGAAGATAGA CCGGCATGGA AATGCTGGGG TTGGACTCTG TGGGATACCG CTGTGTTTCA
     4621 AGGCAAACAT CGCCACGGGT GTGTTCCCCA CGAGTGCGGC CACGCCAGCC TTGATTAACC
     4681 ACCTTCCTAA AATCCCATCC CGCGTAGCAG AAAGGCTGTT TTCCGCAGGC GCTCTTCCAG
     4741 GGGCTTCCGG AAATATGCAC GAGCTTAGTT TTGGCATTAC TAGCAACAAC TATGCTACTG
     4801 GGGCCGTGCG CAACCCATGG AACCCAGACC TCATTCCGGG TGGGAGTTCT GGCGGTGTCG
     4861 CCGCCGCGGT AGCGTCACGC TTGATGCTGG GGGGAATCGG AACCGACACA GGGGCCAGCG
     4921 TAAGGTTGCC AGCAGCCCTG TGTGGCGTCG TAGGCTTCCG ACCTACACTC GGACGCTACC
     4981 CGGGCGACCG CATTATCCCT GTTAGTCCCA CTCGCGATAC TCCTGGTATT ATCGCTCAAT
     5041 GTGTGGCTGA CGTCGTCATC CTGGATCGGA TCATAAGTGG TACACCAGAA CGAATCCCAC
     5101 CGGTTCCGCT TAAAGGCCTC CGGATTGGGC TGCCCACGAC CTATTTTTAT GATGACCTCG
     5161 ATGCCGATGT CGCCTTGGCG GCGGAGACTA CGATTAGACT TCTTGCGAAC AAAGGGGTCA
     5221 CGTTTGTTGA GGCTAACATA CCGCACTTGG ACGAGTTGAA TAAAGGGGCG TCTTTTCCCG
     5281 TGGCACTGTA TGAATTTCCG CACGCTCTTA AACAGTACCT GGACGATTTC GTTAAAACGG
     5341 TTAGCTTTTC AGATGTAATA AAGGGCATCC GATCACCAGA TGTGGCTAAT ATAGCTAATG
     5401 CCCAAATTGA CGGTCATCAA ATTTCCAAAG CCGAGTATGA ACTCGCCCGC CACAGCTTTC
     5461 GGCCAAGACT TCAGGCGACA TATCGAAATT ACTTTAAGCT TAATCGCTTG GATGCGATAC
     5521 TTTTCCCCAC CGCCCCTCTG GTAGCCAGAC CGATTGGTCA AGACTCTTCA GTTATACACA
     5581 ATGGAACTAT GCTTGATACA TTTAAGATAT ATGTACGGAA CGTAGACCCA AGCTCAAATG
     5641 CAGGGCTCCC GGGCTTGTCA ATTCCAGTTT GCCTTACGCC GGACCGGCTT CCAGTGGGGA
     5701 TGGAAATAGA TGGACTTGCG GACTCTGACC AACGCCTTCT CGCTATAGGC GGTGCCCTGG
     5761 AAGAAGCGAT TGGGTTTAGA TATTTCGCTG GACTTCCGAA Cacctagcga atttaaatcg
     5821 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5881 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5941 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6001 gaggattggg aagacaatag caggcatgct ggggacgcgg ccgcaattta acgcgaatta
     6061 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6121 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6181 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     6241 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6301 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     6361 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     6421 atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct
     6481 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     6541 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     6601 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     6661 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     6721 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     6781 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     6841 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     6901 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     6961 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     7021 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     7081 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     7141 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     7201 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     7261 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     7321 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     7381 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     7441 cgacgcccca gcactcgtcc gagggcaaag gaataggtcg acaatcaacc tctggattac
     7501 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7561 tacgctgctt taatgccttt gtatcatgcg ttaactaaac ttgtttattg cagcttataa
     7621 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7681 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa
     7741 atgatgtcaa ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc
     7801 ctgtttaata tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc
     7861 aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag
     7921 ccaggtttag ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt
     7981 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
     8041 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
     8101 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
     8161 gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca
     8221 agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat
     8281 cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc
     8341 ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc
     8401 tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga
     8461 gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa
     8521 tctagctgca tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct
     8581 ggcgctatct gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg
     8641 catggaaaga gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt
     8701 ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc
     8761 aggccacctg ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc
     8821 cgaagcgcat cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc
     8881 agattaatga cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct
     8941 ggatgccgca gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta
     9001 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9061 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9121 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9181 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9241 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9301 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9361 aggccagcaa aaggccagga accgtaaaaa
//