LOCUS       vYM112_PB-pEF1a-optiPIN 9590 bp ds-DNA     circular     11-JUL-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(7923..8511)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(7569..7575)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4521..4547
                     /label="HA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1499..1505
                     /label="TATA box"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(7576..7593)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_signal    6352..6473
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     repeat_region   826..888
                     /label="5'TR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2071..2080
                     /label="SP1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1932..1941
                     /label="SP1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2568..4508
                     /label="optiPIN2"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4839..5147
                     /label="pSV40"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    4509..4520
                     /label="GGSG_Linker1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2189..2198
                     /label="SP1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     enhancer        1064..1295
                     /label="core insul enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2251..2257
                     /label="AP1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(7521..7537)
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     protein_bind    7545..7561
                     /label="lac repressor encoded by lacI lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        1355..2509
                     /label="PEF-1alpha promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(8695..9555)
                     /label="bla"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     misc_feature    5204..6229
                     /label="Hygro"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     680..696
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     misc_feature    1975..1983
                     /label="SP1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(1..57)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_signal    4574..4788
                     /label="BGH polyadenylation sequence"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     enhancer        6491..6721
                     /label="core insul(1) enhancer"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     repeat_region   6996..7030
                     /label="3'TR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(9556..9590)
                     /label="bla promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        1309..1309
                     /label="CMV7 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      complement(83..538)
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    6236..6340
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1562..2500
                     /label="Intron"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(7594..7599)
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2081..2090
                     /label="SP1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(4516..4572)
                     /label="oYM398"
                     /note="sequence: agggataacagggtaatgaggtTTAtgcgtagtctggtacgtcgtacggatagccgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1282..1302
                     /label="oYM001"
                     /note="sequence: ggatacggggaaaaggcctcc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(6445..6504)
                     /label="oYM278"
                     /note="sequence: GGATACGGGGAAAAAGCCATACCAATGGGCCTAAGATACATTGATGAGTTTGGACAAACC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2546..2570
                     /label="oYM397"
                     /note="sequence: taaggtagcgaaggtctgCGTAATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
       61 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
      121 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
      181 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
      241 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
      301 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
      361 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
      421 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
      481 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
      541 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      601 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      661 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt
      721 tttgaacccg tggaggacgg gcagactcgc ggtgcaaatg tgttttacag cgtgatggag
      781 cagatgaaga tgctcgacac gctgcagaac acgcagctag attaacccta gaaagataat
      841 catattgtga cgtacgttaa agataatcat gtgtaaaatt gacgcatgtg ttttatcggt
      901 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      961 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
     1021 acaaaaactc aaaatttctt ctataaagta acaaaacttt tatgagggac agcccccccc
     1081 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     1141 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     1201 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     1261 tgagcctgca gacacctggg gggatacggg gaaaaggcct ccacggccag cacctgcgtg
     1321 acgtacgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     1381 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     1441 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     1501 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     1561 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     1621 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     1681 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     1741 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     1801 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     1861 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     1921 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     1981 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     2041 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     2101 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     2161 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     2221 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     2281 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     2341 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     2401 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     2461 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa ccttcacgca
     2521 ggtggaattc taactataac ggtcctaagg tagcgaaggt ctgCGTAATG ATCACTGGCA
     2581 AAGATATGTA CGATGTCCTT GCTGCAATGG TACCACTCTA TGTAGCAATG ATTCTTGCTT
     2641 ATGGCAGTGT CCGCTGGTGG GGCATATTTA CACCCGATCA GTGCAGCGGG ATCAATCGGT
     2701 TCGTTGCCGT TTTTGCAGTA CCCCTCCTTT CATTCCACTT CATTTCAAGC AATGATCCTT
     2761 ACGCAATGAA TTATCACTTT CTCGCTGCAG ACAGTCTTCA GAAAGTGGTA ATCTTGGCAG
     2821 CACTCTTTCT TTGGCAGGCC TTCTCTAGAA GAGGCTCTTT GGAGTGGATG ATTACCCTCT
     2881 TCTCTCTTTC TACTCTCCCC AACACATTGG TGATGGGCAT ACCTCTTCTT AGAGCAATGT
     2941 ACGGGGATTT CTCTGGCAAC CTCATGGTCC AGATCGTGGT GCTTCAGTCT ATCATATGGT
     3001 ATACCCTTAT GCTGTTCCTT TTTGAGTTCC GCGGGGCAAA ACTGCTCATA AGTGAACAAT
     3061 TCCCAGAGAC CGCCGGGTCT ATCACCAGCT TCCGGGTGGA CTCTGACGTA ATAAGCCTGA
     3121 ACGGGAGAGA ACCCCTTCAA ACAGACGCTG AAATAGGAGA CGATGGTAAG TTGCATGTCG
     3181 TAGTACGGAG GTCAAGCGCT GCTTCCTCAA TGATATCTAG TTTCAACAAA TCCCATGGAG
     3241 GCGGCCTTAA TTCTAGTATG ATAACACCAA GAGCCAGCAA TCTCACAGGC GTAGAAATTT
     3301 ACTCAGTGCA GTCAAGTAGG GAACCAACCC CACGCGCCTC CTCTTTTAAT CAAACTGACT
     3361 TTTATGCCAT GTTTAACGCA AGTAAGGCCC CTAGCCCTAG ACACGGCTAT ACTAATAGTT
     3421 ACGGGGGCGC TGGGGCTGGC CCAGGAGGTG ATGTCTACAG TTTGCAATCA AGTAAGGGGG
     3481 TAACTCCTCG CACTTCAAAC TTTGACGAGG AAGTAATGAA GACTGCCAAG AAAGCAGGAA
     3541 GGGGCGGACG CAGTATGTCA GGGGAACTGT ACAACAATAA CTCAGTTCCT TCTTATCCAC
     3601 CACCTAACCC AATGTTTACA GGCTCAACCT CTGGTGCATC AGGCGTAAAG AAAAAGGAAA
     3661 GCGGTGGAGG CGGCTCCGGA GGTGGCGTCG GCGTAGGAGG CCAGAACAAA GAGATGAACA
     3721 TGTTTGTTTG GAGTTCCAGT GCTAGCCCAG TCAGTGAGGC TAATGCCAAA AACGCAATGA
     3781 CCCGGGGTTC TAGCACAGAT GTTTCTACCG ACCCAAAGGT GTCTATCCCT CCACATGATA
     3841 ATTTGGCAAC CAAAGCCATG CAAAACCTCA TCGAAAATAT GAGTCCTGGC AGAAAGGGCC
     3901 ATGTGGAGAT GGATCAAGAT GGAAACAATG GTGGTAAAAG CCCTTATATG GGGAAAAAAG
     3961 GCTCAGACGT CGAAGATGGC GGACCTGGAC CTAGAAAACA GCAGATGCCC CCAGCTTCCG
     4021 TAATGACACG ACTCATCCTT ATTATGGTGT GGCGGAAACT CATACGCAAT CCAAACACCT
     4081 ACAGCAGCTT GTTTGGGCTC GCCTGGTCTC TTGTATCATT CAAGTGGAAC ATTAAAATGC
     4141 CAACAATAAT GAGCGGGTCT ATCTCTATTC TTTCAGATGC TGGTTTGGGT ATGGCCATGT
     4201 TTAGTTTGGG CCTGTTCATG GCTCTCCAAC CCAAGATAAT TGCTTGTGGA AAGTCTGTAG
     4261 CCGGCTTTGC TATGGCCGTG CGGTTCCTCA CAGGACCTGC CGTAATAGCA GCTACTTCAA
     4321 TCGCCATAGG TATCAGGGGT GACCTGCTTC ACATTGCTAT TGTTCAAGCC GCCTTGCCTC
     4381 AGGGCATCGT ACCTTTCGTC TTTGCTAAGG AATATAACGT TCATCCTGAC ATACTCTCCA
     4441 CCGCTGTCAT CTTCGGGATG TTGGTTGCAT TGCCAGTAAC AGTTCTGTAC TACGTTCTTC
     4501 TTGGCCTTgg cggaagcggc tatccgtacg acgtaccaga ctacgcaTAA acctcattac
     4561 cctgttatcc ctagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4621 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4681 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4741 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggacg cggccgcaat
     4801 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     4861 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4921 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4981 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5041 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     5101 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5161 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatgaaaa agcctgaact
     5221 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     5281 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     5341 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     5401 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     5461 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     5521 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     5581 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     5641 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     5701 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     5761 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     5821 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     5881 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     5941 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     6001 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     6061 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     6121 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     6181 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagg tcgacaatca
     6241 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6301 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat ccctagaatt caacttgttt
     6361 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6421 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaggcccat
     6481 tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     6541 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     6601 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     6661 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     6721 tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
     6781 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
     6841 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
     6901 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
     6961 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
     7021 ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag
     7081 tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg
     7141 cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga
     7201 gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg
     7261 tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt
     7321 ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg
     7381 ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga
     7441 cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg
     7501 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7561 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7621 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7681 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7741 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8821 cgagatccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
//