LOCUS       Exported               10950 bp ds-DNA     circular SYN 27-AUG-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   John Hawkins
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Aug 31, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     mobile_element  152..317
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      complement(383..437)
                     /label=L3S2P24 terminator
                     /note="L3S2P24 terminator"
     CDS             complement(472..561)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(571..4674)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4675..4694
                     /label=""Max Strength RBS"" - SpeI
                     /note="strongest RBS from: Yang et al. Biotechnol Biofuels 
                     (2019) 12:52 
                     https://doi.org/10.1186/s13068-019-1399-6modified by 
                     replacing the last 6 bases with a SpeI site - unclear if 
                     that will cause issues
                     "
     misc_feature    complement(4695..4751)
                     /label=T7A1_O3O4
     protein_bind    4697..4713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4730..4746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(4779..4827)
                     /label=pdhR-aceEF-lpd terminator
     terminator      complement(4828..4880)
                     /label=hisLGDCBHAFI terminator
     promoter        4947..5024
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5025..6107
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     terminator      6199..6252
                     /label=garPLRK-rnpB terminator
     terminator      6253..6309
                     /label=thrLABC terminator
     promoter        6356..6413
                     /label=version C
     misc_feature    6356..6377
                     /label=consensus UP
                     /note="from Estrem et al. 1998 PNAS; Identification of an 
                     UP element consensus sequence forbacterial promoters"
     protein_bind    6383..6402
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_feature    6410..6456
                     /label=sgRNA variable cloning site
     ncRNA           6453..6545
                     /label=optimized sgRNA
                     /ncRNA_class="other"
     terminator      6594..6644
                     /label=L3S1P13 terminator
     terminator      6733..6822
                     /label=spy terminator
     protein_bind    complement(6823..6870)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        6947..6975
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             7058..7747
                     /codon_start=1
                     /label=cat (CmR)
                     /note="cat (Chloramphenicol resistance determinant)"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQFLCMRPIRKPPL
                     PARWPIH"
     protein_bind    complement(7807..7854)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      7905..7991
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8094..8188
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     mobile_element  8237..8434
                     /mobile_element_type="transposon:Tn7R"
                     /label=Tn7R
     promoter        8912..9016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9017..9877
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10178..10566
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            10835..10944
                     /note="incP origin of transfer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcgg gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg
      121 cccaaacata acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg
      181 gaggggtgga aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa
      241 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
      301 atttcactta tctggttctt gaggcggtta aaagagccgt actcttctcc gatgtcgact
      361 aggccatgat gctcattctg tgggaccaaa acgaaaaaac accctttcgg gtgtcttttc
      421 tggaatttgg taccgaggct gcaagtccca ttaaggaggc gcgccttatt acagatcttc
      481 ctcagagatg agcttctgtt ccagatcttc ttctgaaatc aacttttgtt ccagatcttc
      541 ttcagagatg agtttctgct ccgcggccgc gtcacctcct agctgactca aatcaatgcg
      601 tgtttcataa agaccagtga tggattgatg gataagagtg gcatctaaaa cttcttttgt
      661 agacgtatat cgtttacgat caattgttgt atcaaaatat ttaaaagcag cgggagctcc
      721 aagattcgtc aacgtaaata aatgaataat attttctgct tgttcacgta ttggtttgtc
      781 tctatgtttg ttatatgcac taagaacttt atctaaattg gcatctgcta aaataacacg
      841 cttagaaaat tcactgattt gctcaataat ctcatctaaa taatgcttat gctgctccac
      901 aaacaattgt ttttgttcgt tatcttctgg actacccttc aacttttcat aatgactagc
      961 taaatataaa aaattcacat atttgcttgg cagagccagc tcatttcctt tttgtaattc
     1021 tccggcacta gccagcatcc gtttacgacc gttttctaac tcaaaaagac tatatttagg
     1081 tagtttaatg attaagtctt ttttaacttc cttatatcct ttagcttcta aaaagtcaat
     1141 cggatttttt tcaaaggaac ttctttccat aattgtgatc cctagtaact ctttaacgga
     1201 ttttaacttc ttcgatttcc ctttttccac cttagcaacc actaggactg aataagctac
     1261 cgttggacta tcaaaaccac catatttttt tggatcccag tcttttttac gagcaataag
     1321 cttgtccgaa tttctttttg gtaaaattga ctccttggag aatccgcctg tctgtacttc
     1381 tgttttcttg acaatattga cttggggcat ggacaatact ttgcgcactg tggcaaaatc
     1441 tcgcccttta tcccagacaa tttctccagt ttccccatta gtttcgatta gagggcgttt
     1501 gcgaatctct ccatttgcaa gtgtaatttc tgttttgaag aagttcatga tattagagta
     1561 aaagaaatat tttgcggttg ctttgcctat ttcttgctca gacttagcaa tcattttacg
     1621 aacatcataa actttataat caccatagac aaactccgat tcaagttttg gatatttctt
     1681 aatcaaagca gttccaacga cggcatttag atacgcatca tgggcatgat ggtaattgtt
     1741 aatctcacgt actttataga attggaaatc ttttcggaag tcagaaacta atttagattt
     1801 taaggtaatc actttaacct ctcgaataag tttatcattt tcatcgtatt tagtattcat
     1861 gcgactatcc aaaatttgtg ccacatgctt agtgatttgg cgagtttcaa ccaattggcg
     1921 tttgataaaa ccagctttat caagttcact caaacctcca cgttcagctt tcgttaaatt
     1981 atcaaactta cgttgagtga ttaacttggc gtttagaagt tgtctccaat agtttttcat
     2041 ctttttgact acttcttcac ttggaacgtt atccgattta ccacgatttt tatcagaacg
     2101 cgttaagacc ttattgtcta ttgaatcgtc tttaaggaaa ctttgtggaa caatggcatc
     2161 gacatcataa tcacttaaac gattaatatc taattcttgg tccacataca tgtctcttcc
     2221 attttggaga taatagagat agagcttttc attttgcaat tgagtatttt caacaggatg
     2281 ctctttaaga atctgacttc ctaattcttt gataccttct tcgattcgtt tcatacgctc
     2341 tcgcgaattt ttctggccct tttgagttgt ctgattttca cgtgccattt caataacgat
     2401 attttctggc ttatgccgcc ccattacttt gaccaattca tcaacaactt ttacagtctg
     2461 taaaatacct tttttaatag cagggctacc agctaaattt gcaatatgtt catgtaaact
     2521 atcgccttgt ccagacactt gtgctttttg aatgtcttct ttaaatgtca aactatcatc
     2581 atggatcagc tgcataaaat tgcgattggc aaaaccatct gatttcaaaa aatctaatat
     2641 tgttttgcca gattgcttat ccctaatacc attaatcaat tttcgagaca aacgtcccca
     2701 accagtataa cggcgacgtt taagctgttt catcacctta tcatcaaaga ggtgagcata
     2761 tgttttaagt ctttcctcaa tcatctccct atcttcaaat aaggtcaatg ttaaaacaat
     2821 atcctctaag atatcttcat tttcttcatt atccaaaaaa tctttatctt taataatttt
     2881 tagcaaatca tggtaggtac ctaatgaagc attaaatcta tcttcaactc ctgaaatttc
     2941 aacactatca aaacattcta tttttttgaa ataatcttct tttaattgct taacggttac
     3001 ttttcgattt gttttgaaga gtaaatcaac aatggctttc ttctgttcac ctgaaagaaa
     3061 tgctggtttt cgcattcctt cagtaacata tttgaccttt gtcaattcgt tataaaccgt
     3121 aaaatactca taaagcaaac tatgttttgg tagtactttt tcatttggaa gatttttatc
     3181 aaagtttgtc atgcgttcaa taaatgattg agctgaagca cctttatcga caacttcttc
     3241 aaaattccat ggggtaattg tttcttcaga cttccgagtc atccatgcaa aacgactatt
     3301 gccacgcgcc aatggaccaa cataataagg gattcgaaaa gtcaagattt tttcaatctt
     3361 ctcacgattg tcttttaaaa atggataaaa gtcttcttgt cttctcaaaa tagcatgcag
     3421 ctcacccaag tgaatttgat ggggaataga gccgttgtca aaggtccgtt gcttgcgcag
     3481 caaatcttca cgatttagtt tcaccaataa ttcctcagta ccatccattt tttctaaaat
     3541 tggtttgata aatttataaa attcttcttg gctagctccc ccatcaatat aacctgcata
     3601 tccgtttttt gattgatcaa aaaagatttc tttatacttt tctggaagtt gttgtcgaac
     3661 taaagctttt aaaagagtca agtcttgatg atgttcatcg tagcgtttaa tcattgaagc
     3721 tgatagggga gccttagtta tttcagtatt tactcttagg atatctgaaa gtaaaatagc
     3781 atctgataaa ttcttagctg ccaaaaacaa atcagcatat tgatctccaa tttgcgccaa
     3841 taaattatct aaatcatcat cgtaagtatc ttttgaaagc tgtaatttag catcttctgc
     3901 caaatcaaaa tttgatttaa aattaggggt caaacccaat gacaaagcaa tgagattccc
     3961 aaataagcca tttttcttct caccggggag ctgagcaatg agattttcta atcgtcttga
     4021 tttactcaat cgtgcagaaa gaatcgcttt agcatctact ccacttgcgt taatagggtt
     4081 ttcttcaaat aattgattgt aggtttgtac caactggata aatagtttgt ccacatcact
     4141 attatcagga tttaaatctc cctcaatcaa aaaatgacca cgaaacttaa tcatatgcgc
     4201 taaggccaaa tagattaagc gcaaatccgc tttatcagta gaatctacca atttttttcg
     4261 cagatgatag atagttggat atttctcatg ataagcaact tcatctacta tatttccaaa
     4321 aataggatga cgttcatgct tcttgtcttc ttccaccaaa aaagactctt caagtcgatg
     4381 aaagaaacta tcatctactt tcgccatctc atttgaaaaa atctcctgta gataacaaat
     4441 acgattcttc cgacgtgtat accttctacg agctgtccgt ttgagacgag tcgcttccgc
     4501 tgtctctcca ctgtcaaata aaagagcccc tataagattt tttttgatac tgtggcggtc
     4561 tgtatttccc agaaccttga actttttaga cggaacctta tattcatcag tgatcaccgc
     4621 ccatccgacg ctatttgtgc cgatagctaa gcctattgag tatttcttat ccatactagt
     4681 cctccttctc gctcaattgt tatccgctca caattgaatc taagtatcat tgttatccgc
     4741 tcacaagtca acactctttt tgataaattt tactagtctg cagacgtaaa aaaagcggcg
     4801 tggttagccg cttttttaat tgccggagcg caataaaaaa gcccccggaa ggtgatcttc
     4861 cgggggcttt ctcatgcgtt gcttgtattg aaaatggggg ccatgcctac ctatctgcct
     4921 cccgggaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta
     4981 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
     5041 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     5101 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     5161 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     5221 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     5281 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     5341 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     5401 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     5461 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     5521 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     5581 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     5641 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     5701 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     5761 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     5821 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     5881 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     5941 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     6001 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6061 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6121 aatgtaagtt agctcactca ttaggcaccc cacccgggtc cttgagcccc attatcacct
     6181 ccaaagcctt ctcgtctggt cagtttcacc tgttttacgt aaaaacccgc ttcggcgggt
     6241 ttttactttt ggggaaacac agaaaaaagc ccgcacctga cagtgcgggc tttttttttc
     6301 gaccaaaggt gcgactactc ttgcctacta cctatcgact gagctgaaag aattcggaaa
     6361 attttttttc aaaagtactt gaaattgtga gcgctcacaa ttataattct agtagagacc
     6421 aactttggtc tccaccatag cggtcggtct ctgtttaaga gctatgctgg aaacagcata
     6481 gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     6541 tttttttgaa ttcatgtggc tgaccgttct gttgtctctc gctcttccga gtagacgaac
     6601 aataaggcct ccctaacggg gggccttttt tattgataac aaaagtcagt gcttccgcta
     6661 tttccaaaat accgggctaa tacggtttaa acgacctcct ggatttgctc agacagcctt
     6721 ttcgtcattc gtttcagcca aaaaacttaa gaccgccggt cttgtccact accttgcagt
     6781 aatgcggtgg acaggatcgg cggttttctt ttctcttctc aagaagttcc tatactttct
     6841 agagaatagg aacttcggaa taggaacttc ctcctgaacg gccataagaa cgaaggctgt
     6901 ctgttgaact ctcgagccgt ggaaacggat gaaggcacga acccagttga cataagcctg
     6961 ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca tcactaccgg gcgtattttt
     7021 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     7081 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     7141 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     7201 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     7261 tgctcatccg gagttccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     7321 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     7381 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     7441 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     7501 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     7561 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     7621 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     7681 acaacagttt ttatgcatgc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7741 tcattaatgc agctggcacg acaggtttcc cgactggaac tcgagcatca gcttcaaaag
     7801 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggta cttcaagatc
     7861 cccaattcga gctcatcgtc cgggccgcaa gctcctagcg gcggatttgt cctactcagg
     7921 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     7981 cgttttattt gatgcctcaa gctagagagt cattacccca ggcgtttaag ggcaccaata
     8041 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtctagct tggattctca
     8101 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     8161 gtcattactg gatctatcaa caggagtcca agctcagcta attaagctag cttatcgata
     8221 ccgtcgacct cgaaccccac gcccctcttt aatacgacgg gcaatttgca cttcagaaaa
     8281 tgaagagttt gctttagcca taacaaaagt ccagtatgct ttttcacagc ataactggac
     8341 tgatttcagt ttacaactat tctgtctagt ttaagacttt attgtcatag tttagatcta
     8401 ttttgttcag tttaagactt tattgtccgc ccacacccgc ttacgcaggg catccattta
     8461 ttactcaacc gtaaccgatt ttgccaggtt acgcggctgg tcctctagct ggcgtaatag
     8521 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     8581 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     8641 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     8701 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8761 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     8821 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     8881 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8941 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9001 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9061 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9121 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9181 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9241 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9301 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9361 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9421 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9481 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9541 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9601 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9661 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9721 cggtgagcgt gggtcccgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9781 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9841 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9901 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     9961 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10021 ccccgtagaa aagatcaaag gcagttattg gtgcctcact gattaagcat tggtaactgt
    10081 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttttgc
    10141 ggccgcaaga tccggccacg atgcgtccgg cgtagaggat ctgaagatca gcagttcaac
    10201 ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat gtttaacgta
    10261 ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc tctcatggct
    10321 aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa taaaattaat
    10381 ataaatcagc aacttaaata gcctctaagg ttttaagttt tataagaaaa aaaagaatat
    10441 ataaggcttt taaagctttt aaggtttaac ggttgtggac aacaagccag ggatgtaacg
    10501 cactgagaag cccttagagc ctctcaaagc aattttgagt gacacaggaa cacttaacgg
    10561 ctgacatggg aattagccat ggcatcacag tatcgtgatg acagaggcag ggagtgcggc
    10621 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    10681 aggggattcc ttaaggtata ctttccgctg cataaccctg cttcggggtc attatagcga
    10741 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
    10801 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
    10861 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
    10921 atcctgctct gcgaggctgg ccgataagct
//
