LOCUS       Exported               10615 bp ds-DNA     circular SYN 19-DEC-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 1, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        286..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        2000..2240
                     /label=U6 Promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        4130..4205
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     misc_feature    4261..4378
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4517..5695
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4748..5686
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             5714..6739
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    6755..7343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7226..7237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7415..7648
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7726..7847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7887..8022
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8043..8061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8071..8087)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8229..8684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8710..8814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8815..9675
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9846..10434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1981 aaccccgagg ggacccagag agggcctatt tcccatgatt ccttcatatt tgcatatacg
     2041 atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa agatattagt
     2101 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     2161 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     2221 atatatcttg tggaaaggac gaaacaccgg agacggttgt aaatgagcac acaaaataca
     2281 catgctaaaa tattatattc tatgaccttt ataaaatcaa ccaaaatctt ctttttaata
     2341 actttagtat caataattag aatttttatg ttcctttttg caaactttta ataaaaatga
     2401 gcaaaataaa aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct ttaataatag
     2461 ctactccacc acttgttcct aagcggtcag ctcctgcttc aatcattttt tgagcatctt
     2521 caaatgttct aactccacca gctgctttaa ctaaagcatt gtctttaaca actgacttca
     2581 ttagtttaac atcttcaaat gttgcacctg attttgaaaa tcctgttgat gttttaacaa
     2641 attctaatcc agcttcaaca gctatttcac aagctttcat gatttcttct tttgttaata
     2701 aacaattttc cataatacat ttaacaacat gtgatccagc tgcttttttt acagctttca
     2761 tgtcttctaa aactaattca taatttttgt cttttaatgc accaatattt aataccatat
     2821 caatttctgt tgcaccatct ttaattgctt cagaaacttc gaatgctttt gtagctgttg
     2881 tgcatgcacc tagaggaaaa cctacaacat ttgttattcc tacatttgtg ccttttaata
     2941 attctttaca atagcttgtt caatatgaat taacacaaac tgttgcaaaa tcaaattcaa
     3001 ttgcttcatc acataattgt ttaatttcag ctttcgtagc atcttgtttt aataatgtgt
     3061 gatctatata tttgtttagt ttcatttttt ctcctatata ttcattttta attttaattc
     3121 tttaataatt tcgtctactt taactttagc gttttgaaca gattcaccaa cacctataaa
     3181 ataaattttt agtttaggtt cagttccact tgggcgaaca gcaaatcatg acttatcttc
     3241 taaataaaat tttagtaagt cttgtcctgg catattatac attccatcga tgtagtcttc
     3301 aacattaaca actttaagtc cagcaatttg agttaagggt gttgctctca atgatttcat
     3361 taatggttca atttttaatt tcttttcttc tggtttaaaa ttcaagttta aagtgaaagt
     3421 gtaatatgca cccatttctt taaataaatc ttctaaatag tctactaatg ttttattttg
     3481 ttttttataa aatcaagcag cctctgctat taatatagaa gcttgtattc catctttatc
     3541 tctagctgag tcatcaatta catatccata actttcttca taagcaaaaa caaaatttaa
     3601 tccgttatct tcttctttag caatttctct acccattcat ttaaatccag ttaaagtttt
     3661 tacaatatta actccatatt tttcatgagc gattctatca cccaaatcac ttgttacaaa
     3721 acttgaatat agagccggat tttttggaat gctatttaag cgttttagat ttgataattt
     3781 tcaatcaatt aaaattggtc ctgtttgatt tccatctaat cttacaaaat gaccatcatg
     3841 ttttattgcc attccaaatc tgtcagcatc tgggtcattc ataataataa tatctgcatc
     3901 atgtttaata ccatattcaa gcggtatttt tcatgcagga tcaaattctg gatttggatt
     3961 tacaacattt ttaaatgttt catcttcaaa tgcatgctct tcaacctcaa taacgttata
     4021 tcctgattca cgtaatattt ttggggtaaa tttagttcct gttccattaa ctgcgctaaa
     4081 aataattttt aaatcttttt tagcttcttg ctcttttttg tacgtctctg ttttagagct
     4141 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     4201 ggtgcttttt taagcttggc gtaactagat cttgagacaa atggcagtat tcatccacaa
     4261 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4321 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     4381 ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg cccgggtgca
     4441 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
     4501 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     4561 ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg
     4621 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     4681 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     4741 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     4801 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     4861 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     4921 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     4981 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     5041 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     5101 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     5161 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     5221 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     5281 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     5341 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     5401 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     5461 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     5521 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     5581 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     5641 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgacgtac
     5701 ggccaccttc gaaatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     5761 cgaaaagttc gacagcgtat ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     5821 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     5881 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     5941 agtgcttgac attggggaat ttagcgagag cctgacctat tgcatctccc gccgtgcaca
     6001 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     6061 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     6121 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     6181 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     6241 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     6301 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     6361 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     6421 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     6481 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     6541 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     6601 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     6661 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     6721 tccgagggca aaggaataga cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     6781 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6841 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6901 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6961 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7021 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7081 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7141 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7201 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7261 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7321 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     7381 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7441 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7501 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7561 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7621 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     7681 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7741 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7801 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7861 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7921 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7981 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     8041 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     8101 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     8161 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     8221 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8281 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     8341 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8401 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     8461 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8521 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     8581 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     8641 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     8701 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8761 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8821 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8881 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8941 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9001 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     9061 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9121 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     9181 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     9241 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     9301 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     9361 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     9421 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     9481 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9541 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9601 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9661 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9721 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9781 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9841 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9901 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9961 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10021 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10081 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10141 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10201 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10261 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10321 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10381 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10441 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10501 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    10561 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
