LOCUS       Exported                5930 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..894
                     /label=I1L
                     /note="Left homology arm targeting the LAC4 locus"
     misc_feature    895..920
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             925..1950
                     /codon_start=1
                     /label=GFP drop-out
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1955..2144
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2149..3258
                     /label=ScHIS3
                     /note="label=kanMX - 2141..3531"
     misc_feature    3262..4139
                     /label=I1R
                     /note="Right homology arm targeting the LAC4 locus"
     misc_feature    complement(4140..4147)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4151..5930)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgccgaaa gtgattgaag aaccctcaaa cttgctggat atttccttac
       61 ccttgacctt taggctttca attttaccca acaatttgtc caagacaaat tgcaatccac
      121 tggattcaac tgagacataa cgtttaccgt cgttgatctt ggtaggtttt tctgctgtct
      181 ctgtaacaaa atctggtacc tttaatggaa gttcggcttg gccccaggca atttcatgac
      241 ctgcctttag aacaccagca tcatccttta acacggcaac aacataagtg gtatcagaag
      301 gaatagtaac agattcttct ggctttaaag ctggaacgtc gattgtcttt cccgtgtcct
      361 tgtcgataaa caataagtga tctgtcgtaa tgaagtcgtg cttatttgtg attgttacag
      421 atccgtgcgc aattttaata tgaacgggtt caataacctt cttatactct acaaggcccg
      481 gagtaggatt atgctcactg ttacacaaac catccatgat gaacactccg tcatgaacct
      541 cttccttaaa gtcaccacca taagcataag ctttatgcaa cttaccatct gcagtactaa
      601 catcttcgaa ttcaataccg tgatttgccc attcccagat aaagccacct tggtaaaact
      661 tctccttgta gaacaactct tgatattctt tcaaagaacc cgggccgtta cccattgcat
      721 gaccgtactc acacaagatc aaaggctttt caaacttacc attttcatca gtgtggttct
      781 tcctccacct ttccataatt tcaaatgttg ggtacatgaa actaaagata tctgcactca
      841 aagcgttcaa gtcaccctca taatgcacca gtctggtagg atccaattgc ttaagaattc
      901 gcatctagac tgatgagacg aacgtgagac cgaaagtgaa acgtgatttc atgcgtcatt
      961 ttgaacattt tgtaaatctt atttaataat gtgtgcggca attcacattt aatttatgaa
     1021 tgttttctta acatcgcggc aactcaagaa acggcaggtt cggatcttag ctactagaga
     1081 aagaggagaa atactagatg cgtaaaggcg aagagctgtt cactggtgtc gtccctattc
     1141 tggtggaact ggatggtgat gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag
     1201 gtgacgcaac taatggtaaa ctgacgctga agttcatctg tactactggt aaactgccgg
     1261 ttccttggcc gactctggta acgacgctga cttatggtgt tcagtgcttt gctcgttatc
     1321 cggaccatat gaagcagcat gacttcttca agtccgccat gccggaaggc tatgtgcagg
     1381 aacgcacgat ttcctttaag gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg
     1441 aaggcgatac cctggtaaac cgcattgagc tgaaaggcat tgactttaaa gaggacggca
     1501 atatcctggg ccataagctg gaatacaatt ttaacagcca caatgtttac atcaccgccg
     1561 ataaacaaaa aaatggcatt aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca
     1621 gcgtgcagct ggctgatcac taccagcaaa acactccaat cggtgatggt cctgttctgc
     1681 tgccagacaa tcactatctg agcacgcaaa gcgttctgtc taaagatccg aacgagaaac
     1741 gcgatcatat ggttctgctg gagttcgtaa ccgcagcggg catcacgcat ggtatggatg
     1801 aactgtacaa atgaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1861 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1921 gggtgggcct ttctgcgttt ataggtctca gctggaaatc tgctcgtcag tggtgctcac
     1981 actgacgaat catgtacaga tcataccgat gactgcctgg cgactcacaa ctaagcaaga
     2041 cagccggaac cagcgccggc gaacaccact gcatatatgg catatcacaa cagtccacgt
     2101 ctcaagcagt tacagagatg ttacgaacca ctagtgcact gcagtacaaa cacagtcctt
     2161 tcccgcaatt ttctttttct attactcttg gcctcctcta gtacactcta tattttttta
     2221 tgcctcggta atgattttca tttttttttt tccacctagc ggatgactct ttttttttct
     2281 tagcgattgg cattatcaca taatgaatta tacattatat aaagtaatgt gatttcttcg
     2341 aagaatatac taaaaaatga gcaggcaaga taaacgaagg caaagatgac agagcagaaa
     2401 gccctagtaa agcgtattac aaatgaaacc aagattcaga ttgcgatctc tttaaagggt
     2461 ggtcccctag cgatagagca ctcgatcttc ccagaaaaag aggcagaagc agtagcagaa
     2521 caggccacac aatcgcaagt gattaacgtc cacacaggta tagggtttct ggaccatatg
     2581 atacatgctc tggccaagca ttccggctgg tcgctaatcg ttgagtgcat tggtgactta
     2641 cacatagacg accatcacac cactgaggac tgcgggattg ctctcggtca agcttttaaa
     2701 gaggccctag gggccgtgcg tggagtaaaa aggtttggat caggatttgc gcctttggat
     2761 gaggcacttt ccagagcggt ggttgatctt tcgaacaggc cgtacgcagt tgtcgaactt
     2821 ggtttgcaaa gggagaaagt aggtgatctc tcttgcgaga tgatcccgca ttttcttgaa
     2881 agctttgcag aggctagcag aattaccctc cacgttgatt gtctgcgagg caagaatgat
     2941 catcaccgta gtgagagtgc gttcaaggct cttgcggttg ccataagaga agccacctcg
     3001 cccaatggta ccaacgatgt tccctccacc aaaggtgttc ttatgtagtg acaccgatta
     3061 tttaaagctg ctgcatacga tatatataca tgtgtatata tgtataccta tgaatgtcag
     3121 taagtatgta tacgaacagt atgatactga agatgacaag gtaatgcatc attctatacg
     3181 tgtcattctg aacgaggcgc gctttccttt tttctttttg ctttttcttt ttttttctct
     3241 tgaactcgac ggatcataga gtgcttaatt agcttgtaca tggctttgtg gtttctgcca
     3301 taacaagctt cgttacccaa ggaccagata ataatcgaag gatgattgac atctcttagg
     3361 acaagttggg aagctctgtc taagtacgcg acctcgtact ctggattatc tgataagtaa
     3421 tgggcattaa catcgtagag tttatttttg gtatctggat attcagcctc caagttcgta
     3481 tgacgattaa atggctcttg aacaccgtga gtttcaagat ctgcctcatc aatgacccag
     3541 aagcctagtt tatcgaagag gtcatacacc ttaggatggt ttggataatg cgagttacga
     3601 acagcattga tgttaaactt cttcattaga atcaagtctc taacaacaaa atctaatggc
     3661 acagctctac cgaaccttgg atggtgatca tgcctgttga cacctctaaa gagaatgtct
     3721 ttgccattaa cagtaatgtt accgtccttc aactccactt gtctgaaacc aacatggtgc
     3781 ttaatagatt gaatcacact gccatcagat ccaattaaat ccaactggta cttgtacaaa
     3841 gtaggatttt ctgcggtcca atgttctggg gccttgacgt tgatcttgaa agctgtttct
     3901 tcgtcctttt tggtcgaaaa ggaaataaat tctttagttg aaaacgtcgt gttcccattc
     3961 tcctcgttca acaaagagct tgcatcgtaa actttagatc catcttcagg ttcgtaaagt
     4021 gtgaaattga tgtgatcata agaagaaccc tggacatcaa ctttcacaga aagctctgca
     4081 tcctgatact gagagtccac aaaagttgta gtgaccctaa cgtcttcaat atgggccttg
     4141 gcgcgcctca gccgaccgag cggccgcgtg ttacaaccaa ttaaccaatt ctgattagaa
     4201 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     4261 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     4321 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     4381 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     4441 cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt
     4501 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     4561 agcgaggcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     4621 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     4681 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     4741 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     4801 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     4861 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     4921 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctgga
     4981 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     5041 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     5101 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagtc
     5161 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5221 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5281 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5341 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5401 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5461 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5521 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5581 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5641 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5701 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5761 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5821 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5881 atgttctttc ctgcgttatc ccctgattct gtggataacc gtgcggccgc
//
