LOCUS       Exported                2939 bp ds-DNA     circular SYN 08-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    2 pCOLI_A_His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     40..70
                     /label=oWL746
                     /note="Description Gene Use Legacy Name"
     primer_bind     40..59
                     /label=oWL228
                     /note="Description:T7_f Gene:T7 promotor Use:Sequencing 
                     Legacy Name:T7_f"
     promoter        40..58
                     /label=T7 promoter
     primer_bind     complement(88..128)
                     /label=oWL721
                     /note="Description Gene Use Legacy Name"
     RBS             96..118
                     /label=RBS
     primer_bind     complement(135..155)
                     /label=oWL738
                     /note="Description Gene Use Legacy Name"
     CDS             138..155
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     138..155
                     /label=oWL826
                     /note="Description:His Tag forward Gene Use:Sequencing 
                     Legacy Name"
     primer_bind     156..185
                     /label=oWL736
                     /note="Description Gene Use Legacy Name"
     primer_bind     156..179
                     /label=oWL729
                     /note="Description Gene Use Legacy Name"
     primer_bind     167..190
                     /label=oWL740
                     /note="Description Gene Use Legacy Name"
     primer_bind     168..192
                     /label=oWL623
                     /note="Description:SNAP_no_Bam_f Gene Use Legacy Name"
     CDS             168..191
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     168..190
                     /label=oWL744
                     /note="Description Gene Use Legacy Name"
     primer_bind     complement(170..191)
                     /label=oWL678
                     /note="Description:Rv_C-MBP Gene:MBP Use:Cloning Legacy 
                     Name"
     primer_bind     complement(170..191)
                     /label=oWL013
                     /note="Spo11_GST_rev"
     primer_bind     complement(170..191)
                     /label=oWL016
                     /note="Spo11_38_GST_rev"
     primer_bind     175..215
                     /label=oWL804
                     /note="Description:Correct_3C_fwd Gene Use Legacy Name"
     primer_bind     complement(214..238)
                     /label=oWL622
                     /note="Description:pLIB_Strep_mutate_rev Gene Use Legacy 
                     Name"
     primer_bind     complement(266..290)
                     /label=oWL640
                     /note="AB5"
     primer_bind     297..326
                     /label=oWL734
                     /note="Description Gene Use Legacy Name"
     primer_bind     297..316
                     /label=oWL772
                     /note="Description:CTag_backbone_fwd Gene Use:Cloning 
                     Legacy Name"
     primer_bind     complement(384..402)
                     /label=oWL227
                     /note="Description:STO720 (T7r) Gene:T7 terminator (pST44) 
                     Use:Sequencing Legacy Name:STO720"
     terminator      398..445
                     /label=T7 terminator
     primer_bind     complement(409..451)
                     /label=oWL745
                     /note="Description Gene Use Legacy Name"
     promoter        510..601
                     /label=AmpR promoter
     primer_bind     1100..1128
                     /label=oWL481
                     /note="Description:Amp_fwd Gene Use:Mutagenesis Legacy 
                     Name"
     primer_bind     complement(1100..1128)
                     /label=oWL480
                     /note="Description:Amp_rev Gene Use:Mutagenesis Legacy 
                     Name"
     terminator      1466..1505
                     /label=fd terminator
     rep_origin      1536..2124
                     /label=ori
     misc_feature    2310..2449
                     /label=bom
     CDS             complement(2551..2742)
                     /label=rop
ORIGIN
        1 agatgtaggt gttggcacca tgcatcgatc ccgcgaaatt aatacgactc actataggga
       61 gaccacaacg gtttccctcc ggctccggaa ataattttgt ttaactttaa gaaggagata
      121 tacatatggg cagcagccat catcatcatc atcacagcag cggatctctg gaagttctgt
      181 tccaggggcc cggatcccgg tccgacatat gagcgcgcgg aattcaaagg cctacgtcga
      241 cgagctcact agtcgcggcc gctttcgaat ctagagcctg cagtctcgac aagcttgtcg
      301 agaagtacta gaggatgtac aacgcgtgcg cgctgatccg gctgctaaca aagcccgaaa
      361 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      421 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatcgggat tagcccgcct
      481 aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac attcaaatat
      541 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      601 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      661 tgtttttgct cacccagaaa cgctcgtgaa agtaaaagac gcagaggacc aattgggggc
      721 acgagtggga tacatagaac tggacttgaa tagcggtaaa atccttgaga gttttcgccc
      781 tgaagagcgt tttccaatga tgagcacttt caaagttctg ctatgtggag cagtattatc
      841 ccgtgtagat gcggggcaag agcaactcgg acgacgaata cactattcgc agaatgactt
      901 ggttgaatac tccccagtga cagaaaagca ccttacggac ggaatgacgg taagagaatt
      961 atgtagtgcc gccataacga tgagtgataa cactgcggcg aacttacttc tgacaaccat
     1021 cggtggaccg aaggaattaa ccgctttttt gcacaatatg ggagaccatg taactcgcct
     1081 tgaccgttgg gaaccagaac tgaatgaagc cataccaaac gacgagcgag acaccacaat
     1141 gcctgcggca atggcaacaa cattacgcaa actattaact ggcgaactac ttactctggc
     1201 ttcacggcaa caattaatag actggcttga agcggataaa gttgcaggac cactactgcg
     1261 ttcggcactt cctgctggct ggtttattgc tgataaatct ggggcaggag agcgtggttc
     1321 acggggtatc attgccgcac ttggaccaga tggtaagcct tcccgtatcg tagttatcta
     1381 cacgacgggt agtcaggcaa ctatggacga acgaaataga cagattgctg aaataggggc
     1441 ttcactgatt aagcattggt aaaccgatac aattaaaggc tccttttgga gccttttttt
     1501 ttggacggac cggtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1681 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2281 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     2341 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     2401 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     2461 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2521 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2581 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     2641 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     2701 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     2761 gggatttctg ttcatggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     2821 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     2881 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaatac
//
