LOCUS       Exported                3557 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     134..164
                     /label=oWL746
                     /note="Description Gene Use Legacy Name"
     primer_bind     134..153
                     /label=oWL228
                     /note="Description:T7_f Gene:T7 promotor Use:Sequencing 
                     Legacy Name:T7_f"
     promoter        134..152
                     /label=T7 promoter
     primer_bind     complement(182..222)
                     /label=oWL721
                     /note="Description Gene Use Legacy Name"
     RBS             190..212
                     /label=RBS
     primer_bind     219..267
                     /label=oWL482
                     /note="Description:GST_fwdf Gene:GST Use:Mutagenesis Legacy
                     Name"
     CDS             220..873
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(220..256)
                     /label=oWL668
                     /note="Description:GST_D7Y_rev Gene:GST Use:Mutagenesis 
                     Legacy Name"
     primer_bind     complement(220..243)
                     /label=oWL220
                     /note="Description:GST - bp 1-24 - reverse Gene 
                     Use:Sequencing Legacy Name"
     primer_bind     223..252
                     /label=oWL769
                     /note="Description:3C_C-GST_fwd Gene:GST Use:Cloning Legacy
                     Name"
     primer_bind     223..252
                     /label=oWL770
                     /note="Description:C-GST_fwd Gene:GST Use:Cloning Legacy 
                     Name"
     primer_bind     229..265
                     /label=oWL669
                     /note="Description:GST_D7Y_fwd Gene:GST Use:Mutagenesis 
                     Legacy Name"
     primer_bind     complement(255..277)
                     /label=oWL534
                     /note="Description:GST_rev Gene:GST Use:Sequencing Legacy 
                     Name"
     misc_feature    304..306
                     /label=D29E point mutation (gaa)
     primer_bind     831..853
                     /label=oWL132
                     /note="GST_F"
     primer_bind     complement(856..874)
                     /label=oWL771
                     /note="Description:C-GST_rev Gene:GST Use:Cloning Legacy 
                     Name"
     primer_bind     879..902
                     /label=oWL740
                     /note="Description Gene Use Legacy Name"
     primer_bind     879..897
                     /label=oWL736
                     /note="Description Gene Use Legacy Name"
     primer_bind     880..904
                     /label=oWL623
                     /note="Description:SNAP_no_Bam_f Gene Use Legacy Name"
     CDS             880..903
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     880..902
                     /label=oWL744
                     /note="Description Gene Use Legacy Name"
     primer_bind     complement(882..903)
                     /label=oWL678
                     /note="Description:Rv_C-MBP Gene:MBP Use:Cloning Legacy 
                     Name"
     primer_bind     complement(882..903)
                     /label=oWL013
                     /note="Spo11_GST_rev"
     primer_bind     complement(882..903)
                     /label=oWL016
                     /note="Spo11_38_GST_rev"
     misc_feature    889..909
                     /label=fwd primer overhang
     primer_bind     complement(926..950)
                     /label=oWL622
                     /note="Description:pLIB_Strep_mutate_rev Gene Use Legacy 
                     Name"
     primer_bind     complement(978..1002)
                     /label=oWL640
                     /note="AB5"
     primer_bind     1009..1038
                     /label=oWL734
                     /note="Description Gene Use Legacy Name"
     primer_bind     1009..1028
                     /label=oWL772
                     /note="Description:CTag_backbone_fwd Gene Use:Cloning 
                     Legacy Name"
     primer_bind     complement(1096..1114)
                     /label=oWL227
                     /note="Description:STO720 (T7r) Gene:T7 terminator (pST44) 
                     Use:Sequencing Legacy Name:STO720"
     terminator      1110..1157
                     /label=T7 terminator
     primer_bind     complement(1121..1163)
                     /label=oWL745
                     /note="Description Gene Use Legacy Name"
     promoter        1222..1313
                     /label=AmpR promoter
     primer_bind     1812..1840
                     /label=oWL481
                     /note="Description:Amp_fwd Gene Use:Mutagenesis Legacy 
                     Name"
     primer_bind     complement(1812..1840)
                     /label=oWL480
                     /note="Description:Amp_rev Gene Use:Mutagenesis Legacy 
                     Name"
     terminator      2178..2217
                     /label=fd terminator
     rep_origin      2248..2836
                     /label=ori
     misc_feature    3022..3161
                     /label=bom
     CDS             complement(3263..3454)
                     /label=rop
ORIGIN
        1 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
       61 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttggc accatgcatc
      121 gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctccggctcc
      181 ggaaataatt ttgtttaact ttaagaagga gatatacata tgtcccctat actaggttat
      241 tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct tgaagaaaaa
      301 tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa
      361 ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa attaacacag
      421 tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa
      481 gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata cggtgtttcg
      541 agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag caagctacct
      601 gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa tggtgatcat
      661 gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata catggaccca
      721 atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga agctatccca
      781 caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca gggctggcaa
      841 gccacgtttg gtggtggcga ccatcctcca aaatcggatc tggaagttct gttccagggg
      901 cccggatccc ggtccgacat atgagcgcgc ggaattcaaa ggcctacgtc gacgagctca
      961 ctagtcgcgg ccgctttcga atctagagcc tgcagtctcg acaagcttgt cgagaagtac
     1021 tagaggatgt acaacgcgtg cgcgctgatc cggctgctaa caaagcccga aaggaagctg
     1081 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     1141 tcttgagggg ttttttgctg aaaggaggaa ctatatcggg attagcccgc ctaatgagcg
     1201 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1261 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1321 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1381 ctcacccaga aacgctcgtg aaagtaaaag acgcagagga ccaattgggg gcacgagtgg
     1441 gatacataga actggacttg aatagcggta aaatccttga gagttttcgc cctgaagagc
     1501 gttttccaat gatgagcact ttcaaagttc tgctatgtgg agcagtatta tcccgtgtag
     1561 atgcggggca agagcaactc ggacgacgaa tacactattc gcagaatgac ttggttgaat
     1621 actccccagt gacagaaaag caccttacgg acggaatgac ggtaagagaa ttatgtagtg
     1681 ccgccataac gatgagtgat aacactgcgg cgaacttact tctgacaacc atcggtggac
     1741 cgaaggaatt aaccgctttt ttgcacaata tgggagacca tgtaactcgc cttgaccgtt
     1801 gggaaccaga actgaatgaa gccataccaa acgacgagcg agacaccaca atgcctgcgg
     1861 caatggcaac aacattacgc aaactattaa ctggcgaact acttactctg gcttcacggc
     1921 aacaattaat agactggctt gaagcggata aagttgcagg accactactg cgttcggcac
     1981 ttcctgctgg ctggtttatt gctgataaat ctggggcagg agagcgtggt tcacggggta
     2041 tcattgccgc acttggacca gatggtaagc cttcccgtat cgtagttatc tacacgacgg
     2101 gtagtcaggc aactatggac gaacgaaata gacagattgc tgaaataggg gcttcactga
     2161 ttaagcattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacgg
     2221 accggtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2281 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2341 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2401 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2461 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2521 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2581 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2641 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2701 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2761 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2821 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2881 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2941 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3001 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3061 ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3121 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     3181 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     3241 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     3301 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     3361 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     3421 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     3481 tgttcatggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     3541 tgatgaacat gcccggt
//
