LOCUS       Exported                2990 bp ds-DNA     circular SYN 11-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    456..595
                     /label=bom
     CDS             complement(697..888)
                     /label=rop
     promoter        1125..1143
                     /label=T7 promoter
     RBS             1181..1203
                     /label=RBS
     CDS             1304..1327
                     /label=HRV 3C site
     terminator      1534..1581
                     /label=T7 terminator
     promoter        1646..1737
                     /label=AmpR promoter
     terminator      2602..2641
                     /label=fd terminator
     rep_origin      join(2672..2990,1..270)
                     /label=ori
ORIGIN
        1 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
       61 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      121 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      181 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      241 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      301 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      361 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
      421 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
      481 catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg
      541 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
      601 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      661 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      721 gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat
      781 gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct
      841 tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt
      901 gtaaggggga tttctgttca tggggtaatg ataccgatga aacgagagag gatgctcacg
      961 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     1021 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     1081 aatacagatg taggtgttgg caccatgcat cgatcccgcg aaattaatac gactcactat
     1141 agggagacca caacggtttc cctccggctc cggaaataat tttgtttaac tttaagaagg
     1201 agatatacat atgaagcgac gatggaaaaa gaatttcata gccgtctcag cagccaaccg
     1261 ctttaagaaa atctcatcct ccggggcact tagcagcgga tctctggaag ttctgttcca
     1321 ggggcccgga tcccggtccg acatatgagc gcgcggaatt caaaggccta cgtcgacgag
     1381 ctcactagtc gcggccgctt tcgaatctag agcctgcagt ctcgacaagc ttgtcgagaa
     1441 gtactagagg atgtacaacg cgtgcgcgct gatccggctg ctaacaaagc ccgaaaggaa
     1501 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     1561 cgggtcttga ggggtttttt gctgaaagga ggaactatat cgggattagc ccgcctaatg
     1621 agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc aaatatgtat
     1681 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1741 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1801 tttgctcacc cagaaacgct cgtgaaagta aaagacgcag aggaccaatt gggggcacga
     1861 gtgggataca tagaactgga cttgaatagc ggtaaaatcc ttgagagttt tcgccctgaa
     1921 gagcgttttc caatgatgag cactttcaaa gttctgctat gtggagcagt attatcccgt
     1981 gtagatgcgg ggcaagagca actcggacga cgaatacact attcgcagaa tgacttggtt
     2041 gaatactccc cagtgacaga aaagcacctt acggacggaa tgacggtaag agaattatgt
     2101 agtgccgcca taacgatgag tgataacact gcggcgaact tacttctgac aaccatcggt
     2161 ggaccgaagg aattaaccgc ttttttgcac aatatgggag accatgtaac tcgccttgac
     2221 cgttgggaac cagaactgaa tgaagccata ccaaacgacg agcgagacac cacaatgcct
     2281 gcggcaatgg caacaacatt acgcaaacta ttaactggcg aactacttac tctggcttca
     2341 cggcaacaat taatagactg gcttgaagcg gataaagttg caggaccact actgcgttcg
     2401 gcacttcctg ctggctggtt tattgctgat aaatctgggg caggagagcg tggttcacgg
     2461 ggtatcattg ccgcacttgg accagatggt aagccttccc gtatcgtagt tatctacacg
     2521 acgggtagtc aggcaactat ggacgaacga aatagacaga ttgctgaaat aggggcttca
     2581 ctgattaagc attggtaaac cgatacaatt aaaggctcct tttggagcct ttttttttgg
     2641 acggaccggt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2701 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2761 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2821 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2881 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2941 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
//
