LOCUS       Exported                3167 bp ds-DNA     circular SYN 08-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      343..382
                     /label=fd terminator
     rep_origin      413..1001
                     /label=ori
     misc_feature    1187..1326
                     /label=bom
     CDS             complement(1428..1619)
                     /label=rop
     promoter        1856..1874
                     /label=T7 promoter
     RBS             1912..1934
                     /label=RBS
     CDS             1954..1971
                     /label=6xHis
     CDS             1984..2013
                     /label=Myc
     CDS             2020..2049
                     /label=Myc
     CDS             2056..2085
                     /label=Myc
     CDS             2092..2121
                     /label=Myc
     CDS             2128..2157
                     /label=Myc
     CDS             2164..2193
                     /label=Myc
     CDS             2212..2235
                     /label=HRV 3C site
     terminator      2442..2489
                     /label=T7 terminator
     promoter        2554..2645
                     /label=AmpR promoter
ORIGIN
        1 gagcgagaca ccacaatgcc tgcggcaatg gcaacaacat tacgcaaact attaactggc
       61 gaactactta ctctggcttc acggcaacaa ttaatagact ggcttgaagc ggataaagtt
      121 gcaggaccac tactgcgttc ggcacttcct gctggctggt ttattgctga taaatctggg
      181 gcaggagagc gtggttcacg gggtatcatt gccgcacttg gaccagatgg taagccttcc
      241 cgtatcgtag ttatctacac gacgggtagt caggcaacta tggacgaacg aaatagacag
      301 attgctgaaa taggggcttc actgattaag cattggtaaa ccgatacaat taaaggctcc
      361 ttttggagcc tttttttttg gacggaccgg tagaaaagat caaaggatct tcttgagatc
      421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      541 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      781 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1081 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1141 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     1201 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg
     1261 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     1321 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     1381 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     1441 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
     1501 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
     1561 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     1621 gatgcctccg tgtaaggggg atttctgttc atggggtaat gataccgatg aaacgagaga
     1681 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg
     1741 gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc
     1801 agcgcttcgt taatacagat gtaggtgttg gcaccatgca tcgatcccgc gaaattaata
     1861 cgactcacta tagggagacc acaacggttt ccctccggct ccggaaataa ttttgtttaa
     1921 ctttaagaag gagatataca tatgggaagt tctcatcacc accatcatca tagttcgggc
     1981 tccgagcaga aacttatctc tgaggaagac ttaaatgggg agcagaagtt gatctcggag
     2041 gaagatctga atggtgagca aaagttaatc tccgaagaag acttgaatgg cgaacaaaaa
     2101 ctgatctcag aggaagattt gaacggcgaa cagaagttaa tctctgagga ggatctgaac
     2161 ggagaacaga aacttatttc ggaagaagat ttgaacgggt cgagtggtag tctggaagtt
     2221 ctgttccagg ggcccggatc ccggtccgac atatgagcgc gcggaattca aaggcctacg
     2281 tcgacgagct cactagtcgc ggccgctttc gaatctagag cctgcagtct cgacaagctt
     2341 gtcgagaagt actagaggat gtacaacgcg tgcgcgctga tccggctgct aacaaagccc
     2401 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     2461 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcg ggattagccc
     2521 gcctaatgag cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa
     2581 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2641 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2701 ttcctgtttt tgctcaccca gaaacgctcg tgaaagtaaa agacgcagag gaccaattgg
     2761 gggcacgagt gggatacata gaactggact tgaatagcgg taaaatcctt gagagttttc
     2821 gccctgaaga gcgttttcca atgatgagca ctttcaaagt tctgctatgt ggagcagtat
     2881 tatcccgtgt agatgcgggg caagagcaac tcggacgacg aatacactat tcgcagaatg
     2941 acttggttga atactcccca gtgacagaaa agcaccttac ggacggaatg acggtaagag
     3001 aattatgtag tgccgccata acgatgagtg ataacactgc ggcgaactta cttctgacaa
     3061 ccatcggtgg accgaaggaa ttaaccgctt ttttgcacaa tatgggagac catgtaactc
     3121 gccttgaccg ttgggaacca gaactgaatg aagccatacc aaacgac
//
