LOCUS       Exported                2455 bp ds-DNA     circular SYN 08-MAY-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     24..84
                     /label=MCS_insert_fwd
     primer_bind     complement(24..84)
                     /label=Backbone_rev
     primer_bind     54..73
                     /label=oWL228
                     /note="Description:T7_f Gene:T7 promotor Use:Sequencing 
                     Legacy Name:T7_f"
     promoter        54..72
                     /label=T7 promoter
     CDS             complement(54)
                     /label=lacI
     primer_bind     complement(89..132)
                     /label=Rev_NoTag
     RBS             110..132
                     /label=RBS
     CDS             140..223
                     /label=6xHis 3C
     CDS             152..169
                     /label=6xHis
     primer_bind     182..206
                     /label=oWL623
                     /note="Description:SNAP_no_Bam_f Gene Use Legacy Name"
     CDS             182..205
                     /label=HRV 3C site
     primer_bind     complement(184..205)
                     /label=oWL013
                     /note="Spo11_GST_rev"
     primer_bind     complement(184..205)
                     /label=oWL016
                     /note="Spo11_38_GST_rev"
     primer_bind     complement(228..252)
                     /label=oWL622
                     /note="Description:pLIB_Strep_mutate_rev Gene Use Legacy 
                     Name"
     primer_bind     complement(280..304)
                     /label=oWL640
                     /note="AB5"
     primer_bind     311..348
                     /label=pCOLI_CHis_f
     primer_bind     complement(398..416)
                     /label=oWL227
                     /note="Description:STO720 (T7r) Gene:T7 terminator (pST44) 
                     Use:Sequencing Legacy Name:STO720"
     terminator      412..459
                     /label=T7 terminator
     primer_bind     complement(423..499)
                     /label=MCS_insert_rev
     primer_bind     423..499
                     /label=Backbone_fwd
     CDS             complement(630..1421)
                     /label=SmR
     promoter        complement(1422..1513)
                     /label=AmpR promoter
     rep_origin      complement(1561..2299)
                     /direction=LEFT
                     /label=CloDF13 ori
ORIGIN
        1 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctaatacg
       61 actcactata gggagaccac aacggtttcc ctccggctcc ggaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgggcagcag ccatcatcat catcatcaca gcagcggatc
      181 tctggaagtt ctgttccagg ggcccggatc ccggtccgac atatgagcgc gcggaattca
      241 aaggcctacg tcgacgagct cactagtcgc ggccgctttc gaatctagag cctgcagtct
      301 cgacaagctt gtcgagaagt actagaggat gtacaacgcg tgcgcgctga tccggctgct
      361 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      421 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag
      481 aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa
      541 gcggctattt aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg
      601 gcccctcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct
      661 ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct
      721 tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc
      781 tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac
      841 caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag
      901 ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca
      961 aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc
     1021 aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg
     1081 cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg
     1141 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc
     1201 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
     1261 accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg
     1321 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
     1381 gatagttgag tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat
     1441 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1501 aaaaataaac aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg
     1561 gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg
     1621 tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca
     1681 gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg
     1741 aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt
     1801 cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt
     1861 tctcccttac gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt
     1921 gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg
     1981 cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact
     2041 ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg
     2101 ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc
     2161 tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc
     2221 acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc
     2281 tcaagaagat cctttgatct tttctactga accgctctag atttcagtgc aatttatctc
     2341 ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc
     2401 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcg
//
