LOCUS       Exported                6661 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..864
                     /label=I4L
     misc_feature    865..890
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             895..1920
                     /codon_start=1
                     /label=GFP dropout
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1925..2114
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2119..4035
                     /label=AgLEU2
                     /note="label=kanMX - 2141..3531"
     misc_feature    4040..4874
                     /label=I4R
     misc_feature    complement(4875..4882)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4891..6661)
                     /label=KanR/ColE1
ORIGIN
        1 ccctttcagg cgcgcctcat accgtttatg attgcattta cgttactgac cggagttttg
       61 tccgtatttg tgaagccgtt ggagataatt gaggcaccaa ggttcgaacc agactatgct
      121 atattctacc caggaattaa ttgccagtca tgccatgctg ttaaagtacc ccgttcgaaa
      181 cactgttcac tgtgtcggcg atgcattcca ttgcaagatc atcactgcat ttggatcaac
      241 aattgtgttg gctacggtaa ctatgaatac ttctatccct tcctactagc gaactgtacc
      301 attttatcgt atgcatccat cagactattt tcaatgttga gagttctgcg agttgtgagc
      361 aaacatttcc tgtctctctt tttgttaaca acagcgtttg cattacttgc cataatattt
      421 acatactacc agttaaagct cgttcaagaa ggaatgacca acaacgagca agataaatgg
      481 tacctcgtac atgaacatat gagggatgga aaccttttca aggataacaa tggctcactg
      541 tactacagaa ccagttctgg tgtggaagga attgatgaca cattttactc taccaacttg
      601 tacgatcaca gaaagtacaa actcaacaat ccttcgagaa tacacagcgc ggaagaactc
      661 acaaatattt atgatagagg agacttctgg aagaaccttg tagagaggat aagtgtcctt
      721 tgaaaatctg actttaagaa ttctcgtagc tccatgtaca taataaatat agactaataa
      781 gattcgcata acaaactggc ttcaaatcat aatggcaaag aatcacaaaa ttcgagattt
      841 caatctagct aatattccta accggaattc gcatctagac tgatgagacg aacgtgagac
      901 cgaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat
      961 gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa
     1021 acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg cgtaaaggcg
     1081 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
     1141 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
     1201 agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga
     1261 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
     1321 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
     1381 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
     1441 tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg gaatacaatt
     1501 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
     1561 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
     1621 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
     1681 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
     1741 ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc atcaaataaa
     1801 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1861 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggtctca
     1921 gctggaaatc tgctcgtcag tggtgctcac actgacgaat catgtacaga tcataccgat
     1981 gactgcctgg cgactcacaa ctaagcaaga cagccggaac cagcgccggc gaacaccact
     2041 gcatatatgg catatcacaa cagtccacgt ctcaagcagt tacagagatg ttacgaacca
     2101 ctagtgcact gcagtacatc gaggagaact tctagtatat ctacatacct aatattattg
     2161 ccttattaaa aatggaatcc caacaattac atcaaaatcc acattctctt caaaatcaat
     2221 tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta tatttatagg ataattatac
     2281 tctatttctc aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga
     2341 aatatcttga ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga
     2401 atctcttagc aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg
     2461 cacagaatca aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat
     2521 ataccttttt caactgaaaa attgggagaa aaaggaaagg tgagagcgcc ggaaccggct
     2581 tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg
     2641 acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt
     2701 ctaacttttc ttacctttta catttcagca atatatatat atatatttca aggatatacc
     2761 attctaatgt ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa
     2821 atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag
     2881 ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg tgttccactt
     2941 ccagatgagg cgctggaagc ctccaagaag gctgatgccg ttttgttagg tgctgtgggt
     3001 ggtcctaaat ggggaaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa
     3061 gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac
     3121 ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta
     3181 gtgggaggta tttactttgg taagagaaag gaggacgatg gtgatggtgt cgcttgggat
     3241 agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc
     3301 ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct
     3361 tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaatttcc tacattgaag
     3421 gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta
     3481 aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt
     3541 atcccaggtt ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac
     3601 accgcatttg gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag
     3661 gtcaacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg
     3721 cctgaagaag gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga
     3781 actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa
     3841 gttaagaaaa tccttgctta acctggagga cccttctctt tagactattc tactcttatg
     3901 cacgtaaaaa attctaggaa atatgtatta actaggagta aaataaccgg ctagtggcat
     3961 tcatatagcc gtctgtttac atctacatca cacatttcga gtgtatatct cgcaacgttg
     4021 gcgttaaata ggcaggagta attcaaggtt gttcaatatt ccgattgtta gtagattcag
     4081 ttctaccttc gctcagtttt cttaatgact caaaggatat tagaattgaa aaatttcaaa
     4141 aaagctttaa gcatagaacc acgcacttat taaatacaat ggagaaatga tttggtgggc
     4201 aattaccggt ttcaattgac tccgaacagg ttcaaataca ccacatttaa cagactacta
     4261 agaaagagtc aaaaagattt ggctgaacat taagggaggc taggtttttt agtgactttt
     4321 tgcgagagaa acgctttttt agcagattaa gcaataagtt taaacatgtc cgttgaattg
     4381 tccaaagtat ccatcttggg tcaggacacc attcatgtcg gataccagat tcaggaacac
     4441 attgtgaaga ctactctgga acaatgcaaa tcgtcgacat atgttatcat caacgatact
     4501 aatgtctacc aggtgcctta ctaccatgac ttggttgagc tatttgagaa gtctctaccc
     4561 gagacttccc gtttgctcac atatgttgtg aagccaggcg aggctaacaa gacccgtgaa
     4621 actaaggcac agattgaaga ctttatgttg aatgaagggt gcactcgtga taccgtcgtt
     4681 attgcaattg gtggtggtgt gatcggtgat atgattgggt ttgttgctgc gacattcatg
     4741 agaggtgttc gtttcattca aattcctaca tcgctattgg caatggttga ctcgtctatt
     4801 ggtggtaaga ctgccgttga caccccaatc ggtaaaaact tcattggtgc cttctggcaa
     4861 ccacaatttg tgttggcgcg cctcagccga gcggccgcgt gttacaacca attaaccaat
     4921 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     4981 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     5041 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     5101 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     5161 acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca
     5221 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     5281 gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     5341 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     5401 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
     5461 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     5521 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     5581 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     5641 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     5701 cgcggcctgg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
     5761 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
     5821 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
     5881 aaggatcagt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5941 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6001 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6061 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6121 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6181 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6241 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6301 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6361 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6421 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6481 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6541 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6601 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtgcggccg
     6661 c
//
