LOCUS       Exported                2608 bp ds-DNA     circular SYN 08-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(410..501)
                     /label=AmpR promoter
     rep_origin      complement(517..1266)
                     /direction=LEFT
                     /label=RSF ori
     promoter        1462..1480
                     /label=T7 promoter
     RBS             1518..1540
                     /label=RBS
     CDS             1560..1577
                     /label=6xHis
     CDS             1590..1616
                     /label=HA
     CDS             1623..1649
                     /label=HA
     CDS             1656..1682
                     /label=HA
     CDS             1695..1718
                     /label=HRV 3C site
     terminator      1925..1972
                     /label=T7 terminator
     CDS             complement(join(2202..2608,1..409))
                     /label=KanR
ORIGIN
        1 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
       61 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
      121 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
      181 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
      241 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
      301 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
      361 aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata ctcttccttt
      421 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
      481 gtatttagaa aaataaacaa ataggcatgc agcgctcttc cgcttcctcg ctcactgact
      541 cgctacgctc ggtcgttcga ctgcggcgag cggtgtcagc tcactcaaaa gcggtaatac
      601 ggttatccac agaatcaggg gataaagccg gaaagaacat gtgagcaaaa agcaaagcac
      661 cggaagaagc caacgccgca ggcgtttttc cataggctcc gcccccctga cgagcatcac
      721 aaaaatcgac gctcaagcca gaggtggcga aacccgacag gactataaag ataccaggcg
      781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat
      901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1021 ttatcgccac tggcagcagc cattggtaac tgatttagag gactttgtct tgaagttatg
     1081 cacctgttaa ggctaaactg aaagaacaga ttttggtgag tgcggtcctc caacccactt
     1141 accttggttc aaagagttgg tagctcagcg aaccttgaga aaaccaccgt tggtagcggt
     1201 ggtttttctt tatttatgag atgatgaatc aatcggtcta tcaagtcaac gaacagctat
     1261 tccgttactc tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc
     1321 catacgatat aagttgtaat tctcatgtta gtcatgcccc gcgcccaccg gaaggagctg
     1381 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
     1441 cttacattaa ttgcgttgcg ctaatacgac tcactatagg gagaccacaa cggtttccct
     1501 ccggctccgg aaataatttt gtttaacttt aagaaggaga tatacatatg gggagttcgc
     1561 accatcatca ccaccatagc tctggctcct atccgtatga cgtgccagac tatgctggta
     1621 gctatcctta cgatgtcccc gattatgcgg gatcttatcc atacgatgtg ccagactatg
     1681 caagttcagg ttccctggaa gttctgttcc aggggcccgg atcccggtcc gacatatgag
     1741 cgcgcggaat tcaaaggcct acgtcgacga gctcactagt cgcggccgct ttcgaatcta
     1801 gagcctgcag tctcgacaag cttgtcgaga agtactagag gatgtacaac gcgtgcgcgc
     1861 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     1921 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc
     1981 tcaggcattt gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca
     2041 gcaatagaca taagcggcta tttaacgacc ctgccctgaa ccgacgacaa gctgacgacc
     2101 gggtctccgc aagtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2161 tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc
     2221 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2281 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2341 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     2401 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2461 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2521 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     2581 acgcggtcgc tgttaaaagg acaattac
//
