LOCUS       Exported                6358 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..864
                     /label=I4L
     misc_feature    865..890
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             895..1920
                     /codon_start=1
                     /label=GFP dropout
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1925..2114
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2119..3732
                     /label=hphMX
                     /note="hphMX"
     primer_bind     3683..3704
                     /label=K11F
     misc_feature    3737..4571
                     /label=I4R
     misc_feature    complement(4572..4579)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4588..6358)
                     /label=KanR/ColE1
     primer_bind     6289..6306
                     /label=K1F
ORIGIN
        1 ccctttcagg cgcgcctcat accgtttatg attgcattta cgttactgac cggagttttg
       61 tccgtatttg tgaagccgtt ggagataatt gaggcaccaa ggttcgaacc agactatgct
      121 atattctacc caggaattaa ttgccagtca tgccatgctg ttaaagtacc ccgttcgaaa
      181 cactgttcac tgtgtcggcg atgcattcca ttgcaagatc atcactgcat ttggatcaac
      241 aattgtgttg gctacggtaa ctatgaatac ttctatccct tcctactagc gaactgtacc
      301 attttatcgt atgcatccat cagactattt tcaatgttga gagttctgcg agttgtgagc
      361 aaacatttcc tgtctctctt tttgttaaca acagcgtttg cattacttgc cataatattt
      421 acatactacc agttaaagct cgttcaagaa ggaatgacca acaacgagca agataaatgg
      481 tacctcgtac atgaacatat gagggatgga aaccttttca aggataacaa tggctcactg
      541 tactacagaa ccagttctgg tgtggaagga attgatgaca cattttactc taccaacttg
      601 tacgatcaca gaaagtacaa actcaacaat ccttcgagaa tacacagcgc ggaagaactc
      661 acaaatattt atgatagagg agacttctgg aagaaccttg tagagaggat aagtgtcctt
      721 tgaaaatctg actttaagaa ttctcgtagc tccatgtaca taataaatat agactaataa
      781 gattcgcata acaaactggc ttcaaatcat aatggcaaag aatcacaaaa ttcgagattt
      841 caatctagct aatattccta accggaattc gcatctagac tgatgagacg aacgtgagac
      901 cgaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat
      961 gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa
     1021 acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg cgtaaaggcg
     1081 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
     1141 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
     1201 agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga
     1261 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
     1321 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
     1381 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
     1441 tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg gaatacaatt
     1501 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
     1561 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
     1621 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
     1681 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
     1741 ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc atcaaataaa
     1801 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1861 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggtctca
     1921 gctggaaatc tgctcgtcag tggtgctcac actgacgaat catgtacaga tcataccgat
     1981 gactgcctgg cgactcacaa ctaagcaaga cagccggaac cagcgccggc gaacaccact
     2041 gcatatatgg catatcacaa cagtccacgt ctcaagcagt tacagagatg ttacgaacca
     2101 ctagtgcact gcagtacagt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
     2161 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
     2221 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
     2281 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctccagac ctgcgagcag
     2341 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
     2401 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg
     2461 ctaggataca gttctcacat cacatccgaa cataaacaaa aatgggtaaa aagcctgaac
     2521 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtg tccgacctga
     2581 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     2641 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     2701 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa tttagcgaga
     2761 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     2821 ccgaactgcc cgctgttctg caaccggtcg cggaggccat ggatgcgatc gctgcggccg
     2881 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     2941 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     3001 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     3061 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     3121 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     3181 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     3241 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     3301 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     3361 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     3421 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     3481 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaataa agtaactgac
     3541 aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt
     3601 gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc
     3661 ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg
     3721 gtcgctatac tggagtaatt caaggttgtt caatattccg attgttagta gattcagttc
     3781 taccttcgct cagttttctt aatgactcaa aggatattag aattgaaaaa tttcaaaaaa
     3841 gctttaagca tagaaccacg cacttattaa atacaatgga gaaatgattt ggtgggcaat
     3901 taccggtttc aattgactcc gaacaggttc aaatacacca catttaacag actactaaga
     3961 aagagtcaaa aagatttggc tgaacattaa gggaggctag gttttttagt gactttttgc
     4021 gagagaaacg cttttttagc agattaagca ataagtttaa acatgtccgt tgaattgtcc
     4081 aaagtatcca tcttgggtca ggacaccatt catgtcggat accagattca ggaacacatt
     4141 gtgaagacta ctctggaaca atgcaaatcg tcgacatatg ttatcatcaa cgatactaat
     4201 gtctaccagg tgccttacta ccatgacttg gttgagctat ttgagaagtc tctacccgag
     4261 acttcccgtt tgctcacata tgttgtgaag ccaggcgagg ctaacaagac ccgtgaaact
     4321 aaggcacaga ttgaagactt tatgttgaat gaagggtgca ctcgtgatac cgtcgttatt
     4381 gcaattggtg gtggtgtgat cggtgatatg attgggtttg ttgctgcgac attcatgaga
     4441 ggtgttcgtt tcattcaaat tcctacatcg ctattggcaa tggttgactc gtctattggt
     4501 ggtaagactg ccgttgacac cccaatcggt aaaaacttca ttggtgcctt ctggcaacca
     4561 caatttgtgt tggcgcgcct cagccgagcg gccgcgtgtt acaaccaatt aaccaattct
     4621 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     4681 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     4741 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     4801 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     4861 actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc
     4921 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     4981 tgcgcctgag cgaggcgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     5041 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     5101 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
     5161 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     5221 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     5281 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     5341 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     5401 ggcctggagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     5461 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     5521 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
     5581 gatcagtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggccgc
//
