LOCUS       Exported                6156 bp ds-DNA     circular SYN 03-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA.
[LOCUS]: Exported
[ACCESSION]:
            .
[SOURCE]: 1..6176
[CDS]: 2141..3531
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    9..16
                     /label=US AscI site
                     /note="label=US AscI site - 29..36"
     misc_feature    17..866
                     /label=I5L
                     /note="label=I5L - 37..886"
     misc_feature    867..892
                     /label=ConLS1' overhang
                     /note="label=ConLS1' overhang - 887..912"
     CDS             897..1922
                     /codon_start=1
                     /label=GFP dropout
                     /note="label=GFP dropout - 917..1942"
                     /translation="*DRK*NVISCVILNIL*ILFNNVCGNSHLIYECFLNIAATQETAG
                     SDLSY*RKRRNTRCVKAKSCSLVSSLFWWNWMVMSTVISFPCVARVKVTQLMVN*R*SS
                     SVLLVNCRFLGRLW*RR*LMVFSALLVIRTI*SSMTSSSPPCRKAMCRNARFPLRMTAR
                     TKRVRK*NLKAIPW*TALS*KALTLKRTAISWAISWNTILTATMFTSPPINKKMALKRI
                     LKFATTWRMAACSWLITTSKTLQSVMVLFCCQTITI*ARKAFCLKIRTRNAIIWFCWSS
                     *PQRASRMVWMNCTNDQASNKTKGSVERLGLSFYLLFVGERSLLESHWLTFGWAFLRL*
                     VS"
     misc_feature    1927..2116
                     /label=ConRE'
                     /note="label=ConRE' - 1947..2136"
     CDS             2121..3511
                     /label=kanMX
                     /note="label=kanMX - 2141..3531"
     misc_feature    3516..4365
                     /label=I5R
                     /note="label=I5R - 3536..4385"
     misc_feature    complement(4366..4373)
                     /label=DS AscI site
                     /note="label=DS AscI site - complement(4386..4393)"
     CDS             complement(4386..6156)
                     /label=KanR/ColE1
                     /note="label=KanR/ColE1 - complement(4406..6176)"
ORIGIN
        1 ccctttcagg cgcgccggtg ttgcattagt atcgccgtgg atgaagatga aggtggaata
       61 gacgttaccc atagataccc gaatgagtat ctgattgagg atttttgact tacttacttc
      121 ttcgagatac actactctgc cgaaatttcg gactggaatg cgtcaaagtg aatccacttt
      181 gcaggaaaac gcaggaaaaa cggattcgaa aacgcaacaa aaaaaaaaaa attatacata
      241 ataaaatttg aatcaaagca acaagttcac gaatccatta aagcagtcag aatccaggaa
      301 aaaaaagaaa cagagccaga aaaaaggcag caacgaatca caaccacagc gtattgcgtc
      361 agtggaaatt aattaccttg gtatttccaa tgctgtgtac aaccaaaatt tccacatagt
      421 tagttgattc gcatttccct ggtaaccgcc ctcggcaacc tccctcggaa acttccctcc
      481 agagtaactt cccccagagt tatagagata tagagttata gtagagatat agagctatag
      541 atagattgat tcttgttgta gcatttattc cgttaaactt ctgcggctaa ttttgcctag
      601 cttggccttt tttttttgtt tttcgagctt tcaaagacaa atggacattc ttacataata
      661 tattatcata tacaccgaga tcggtgggtc agagcctgta gggatggaca ttttccttct
      721 agactcgcct tccagacctt tttttggttt gtttctctct ccgtggaact cgctttcttt
      781 ttcgccggtg atctcgaaac gtaatcgata tgtattccat tccattcgct attttttttg
      841 ttccataaac catgttctta tgaacagaat tcgcatctag actgatgaga cgaacgtgag
      901 accgaaagtg aaacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
      961 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     1021 aaacggcagg ttcggatctt agctactaga gaaagaggag aaatactaga tgcgtaaagg
     1081 cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg
     1141 tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct
     1201 gaagttcatc tgtactactg gtaaactgcc ggttccttgg ccgactctgg taacgacgct
     1261 gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt
     1321 caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg
     1381 cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga
     1441 gctgaaaggc attgacttta aagaggacgg caatatcctg ggccataagc tggaatacaa
     1501 ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa
     1561 ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca
     1621 aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca
     1681 aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt
     1741 aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgaccag gcatcaaata
     1801 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1861 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttataggtct
     1921 cagctggaaa tctgctcgtc agtggtgctc acactgacga atcatgtaca gatcataccg
     1981 atgactgcct ggcgactcac aactaagcaa gacagccgga accagcgccg gcgaacacca
     2041 ctgcatatat ggcatatcac aacagtccac gtctcaagca gttacagaga tgttacgaac
     2101 cactagtgca ctgcagtaca agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     2161 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     2221 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     2281 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctccagacct gcgagcaggg
     2341 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     2401 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     2461 aggatacagt tctcacatca catccgaaca taaacaaaaa tgggtaagga aaagactcac
     2521 gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     2581 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     2641 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2701 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2761 actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc attccaggta
     2821 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2881 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctg
     2941 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     3001 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
     3061 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     3121 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3181 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3241 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3301 tttttctaaa gtaactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata
     3361 gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt
     3421 tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt
     3481 caatcgtatg tgaatgctgg tcgctatact ggagtttcac aatggaggga gtgaaatcta
     3541 aaagtggaaa aaaaaagggg aggcgaaaaa caaaaaaaaa aaaaagaacg aaccgaacta
     3601 tgtccacagg agcagaagca gaagctgcgg cagcagcttc tgcggctttg aaagaggaaa
     3661 cgcgtagatg aaagaatgat ccaagcccgg ctttccctct gtgcgcggat tgttattttt
     3721 ttagacttat ttctatgttt gttttggacc taatataact tagtcatgaa tcctggttga
     3781 ctcgcgcgca tctttttcca ctctgcgatg attcacagtg cagcgtgcag ctcagctcca
     3841 gcagcagtac cagcggcagc tccaacagtg gagccttcct ttcacgaacg gaatagaaac
     3901 aggaaggaaa aaaatagacc aacaagagga cccatccgcg acttctaatt tttggcgcat
     3961 gaaccgcgct gttctacccg cccgtccgtc ctcatccctc ccccccagga aaacaggaaa
     4021 ttgggaaacc taggtgcgtg cattttcgtt ttgggcgatg attgacaatg agacttagtc
     4081 cgcttggttg ggtttcgttt caaaaacaag gtttggaaga agatcttggg agagaaagac
     4141 cgagagatct tgaccagggc gaagacgacc agtagctttt ggtatagccg ggataagctt
     4201 caggcaccct agggttgtat aagagttgta tgcaaggatg tgcgcaaggg tttctgaagc
     4261 aggatggaaa tgtggtgtgt ttttttgtag agcaattgcc ttaaagtcgg ggattacgcc
     4321 gagacagcag cttgacggtt ccgtggtaat atgtgtccgg caacaggcgc gcctcagccg
     4381 accgagcggc cgcgtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca
     4441 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     4501 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     4561 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     4621 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     4681 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     4741 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg aggcgaaata
     4801 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     4861 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     4921 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     4981 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     5041 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     5101 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     5161 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctggagcaa gacgtttccc
     5221 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     5281 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
     5341 ggctttgttg aataaatcga acttttgctg agttgaagga tcagtcatga ccaaaatccc
     5401 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5461 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5521 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5581 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5641 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5701 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5761 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5821 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5881 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5941 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6001 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6061 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6121 gttatcccct gattctgtgg ataaccgtgc ggccgc
//
