LOCUS       Exported                3263 bp ds-DNA     circular SYN 14-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    626..765
                     /label=bom
     CDS             complement(867..1058)
                     /label=rop
     promoter        1295..1313
                     /label=T7 promoter
     RBS             1351..1373
                     /label=RBS
     CDS             1381..1413
                     /label=6xHis 3C
     CDS             1393..1410
                     /label=6xHis
     CDS             1411..1734
                     /label=TrxA
     CDS             1747..1770
                     /label=HRV 3C site
     terminator      1977..2024
                     /label=T7 terminator
     promoter        2089..2180
                     /label=AmpR promoter
     terminator      3045..3084
                     /label=fd terminator
     rep_origin      join(3115..3263,1..440)
                     /label=ori
ORIGIN
        1 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
       61 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      241 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      601 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
      661 gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
      721 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
      781 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      841 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
      901 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
      961 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     1021 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     1081 tttctgttca tggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
     1141 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
     1201 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
     1261 taggtgttgg caccatgcat cgatcccgcg aaattaatac gactcactat agggagacca
     1321 caacggtttc cctccggctc cggaaataat tttgtttaac tttaagaagg agatatacat
     1381 atgggcagca gccatcatca tcatcatcac agcgataaaa ttattcacct gactgacgac
     1441 agttttgaca cggatgtact caaagcggac ggggcgatcc tcgtcgattt ctgggcagag
     1501 tggtgcggtc cgtgcaaaat gatcgccccg attctggatg aaatcgctga cgaatatcag
     1561 ggcaaactga ccgttgcaaa actgaacatc gatcaaaacc ctggcactgc gccgaaatat
     1621 ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa
     1681 gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg acgctaacct ggccagcagc
     1741 ggatctctgg aagttctgtt ccaggggccc ggatcccggt ccgacatatg agcgcgcgga
     1801 attcaaaggc ctacgtcgac gagctcacta gtcgcggccg ctttcgaatc tagagcctgc
     1861 agtctcgaca agcttgtcga gaagtactag aggatgtaca acgcgtgcgc gctgatccgg
     1921 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
     1981 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
     2041 tatcgggatt agcccgccta atgagcgggc ttttttttaa ttcccctatt tgtttatttt
     2101 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2161 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2221 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg
     2281 cagaggacca attgggggca cgagtgggat acatagaact ggacttgaat agcggtaaaa
     2341 tccttgagag ttttcgccct gaagagcgtt ttccaatgat gagcactttc aaagttctgc
     2401 tatgtggagc agtattatcc cgtgtagatg cggggcaaga gcaactcgga cgacgaatac
     2461 actattcgca gaatgacttg gttgaatact ccccagtgac agaaaagcac cttacggacg
     2521 gaatgacggt aagagaatta tgtagtgccg ccataacgat gagtgataac actgcggcga
     2581 acttacttct gacaaccatc ggtggaccga aggaattaac cgcttttttg cacaatatgg
     2641 gagaccatgt aactcgcctt gaccgttggg aaccagaact gaatgaagcc ataccaaacg
     2701 acgagcgaga caccacaatg cctgcggcaa tggcaacaac attacgcaaa ctattaactg
     2761 gcgaactact tactctggct tcacggcaac aattaataga ctggcttgaa gcggataaag
     2821 ttgcaggacc actactgcgt tcggcacttc ctgctggctg gtttattgct gataaatctg
     2881 gggcaggaga gcgtggttca cggggtatca ttgccgcact tggaccagat ggtaagcctt
     2941 cccgtatcgt agttatctac acgacgggta gtcaggcaac tatggacgaa cgaaatagac
     3001 agattgctga aataggggct tcactgatta agcattggta aaccgataca attaaaggct
     3061 ccttttggag cctttttttt tggacggacc ggtagaaaag atcaaaggat cttcttgaga
     3121 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3181 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3241 agcgcagata ccaaatactg tcc
//
