LOCUS       Exported                3227 bp ds-DNA     circular SYN 14-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      14..602
                     /label=ori
     misc_feature    788..927
                     /label=bom
     CDS             complement(1029..1220)
                     /label=rop
     promoter        1457..1475
                     /label=T7 promoter
     RBS             1513..1535
                     /label=RBS
     CDS             1543..1572
                     /label=6xHis 3C
     CDS             1555..1572
                     /label=6xHis
     CDS             1573..1860
                     /label=SUMO
     CDS             1861..1914
                     /label=6xHis 3C
     CDS             1873..1896
                     /label=HRV 3C site
     terminator      2103..2150
                     /label=T7 terminator
     promoter        2215..2306
                     /label=AmpR promoter
     terminator      3171..3210
                     /label=fd terminator
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      781 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg
      841 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
      901 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
      961 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     1021 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc
     1081 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg
     1141 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
     1201 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catggggtaa
     1261 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     1321 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     1381 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ggcaccatgc
     1441 atcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctccggc
     1501 tccggaaata attttgttta actttaagaa ggagatatac atatgggcag cagccatcat
     1561 catcatcatc actcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
     1621 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
     1681 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt
     1741 aaggaaatgg actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc
     1801 cctgaagatt tggacatgga ggataacgat attattgagg ctcacagaga acagattggt
     1861 agcagcggat ctctggaagt tctgttccag gggcccggat cccggtccga catatgagcg
     1921 cgcggaattc aaaggcctac gtcgacgagc tcactagtcg cggccgcttt cgaatctaga
     1981 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tgtacaacgc gtgcgcgctg
     2041 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     2101 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     2161 gaactatatc gggattagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
     2221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctc gtgaaagtaa
     2401 aagacgcaga ggaccaattg ggggcacgag tgggatacat agaactggac ttgaatagcg
     2461 gtaaaatcct tgagagtttt cgccctgaag agcgttttcc aatgatgagc actttcaaag
     2521 ttctgctatg tggagcagta ttatcccgtg tagatgcggg gcaagagcaa ctcggacgac
     2581 gaatacacta ttcgcagaat gacttggttg aatactcccc agtgacagaa aagcacctta
     2641 cggacggaat gacggtaaga gaattatgta gtgccgccat aacgatgagt gataacactg
     2701 cggcgaactt acttctgaca accatcggtg gaccgaagga attaaccgct tttttgcaca
     2761 atatgggaga ccatgtaact cgccttgacc gttgggaacc agaactgaat gaagccatac
     2821 caaacgacga gcgagacacc acaatgcctg cggcaatggc aacaacatta cgcaaactat
     2881 taactggcga actacttact ctggcttcac ggcaacaatt aatagactgg cttgaagcgg
     2941 ataaagttgc aggaccacta ctgcgttcgg cacttcctgc tggctggttt attgctgata
     3001 aatctggggc aggagagcgt ggttcacggg gtatcattgc cgcacttgga ccagatggta
     3061 agccttcccg tatcgtagtt atctacacga cgggtagtca ggcaactatg gacgaacgaa
     3121 atagacagat tgctgaaata ggggcttcac tgattaagca ttggtaaacc gatacaatta
     3181 aaggctcctt ttggagcctt tttttttgga cggaccggta gaaaaga
//
