LOCUS       Exported                2578 bp ds-DNA     circular SYN 11-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(29..778)
                     /direction=LEFT
                     /label=RSF ori
     promoter        974..992
                     /label=T7 promoter
     CDS             complement(974)
                     /label=lacI
     RBS             1030..1052
                     /label=RBS
     CDS             1060..1104
                     /label=Strep-Tag II
     CDS             1141..1164
                     /label=Strep-Tag II
     CDS             1177..1200
                     /label=HRV 3C site
     terminator      1407..1454
                     /label=T7 terminator
     CDS             complement(1684..2499)
                     /label=KanR
     promoter        complement(join(2500..2578,1..13))
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggcat gcagcgctct tccgcttcct cgctcactga ctcgctacgc
       61 tcggtcgttc gactgcggcg agcggtgtca gctcactcaa aagcggtaat acggttatcc
      121 acagaatcag gggataaagc cggaaagaac atgtgagcaa aaagcaaagc accggaagaa
      181 gccaacgccg caggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      241 acgctcaagc cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcagttc
      421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      541 actggcagca gccattggta actgatttag aggactttgt cttgaagtta tgcacctgtt
      601 aaggctaaac tgaaagaaca gattttggtg agtgcggtcc tccaacccac ttaccttggt
      661 tcaaagagtt ggtagctcag cgaaccttga gaaaaccacc gttggtagcg gtggtttttc
      721 tttatttatg agatgatgaa tcaatcggtc tatcaagtca acgaacagct attccgttac
      781 tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat
      841 ataagttgta attctcatgt tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
      901 gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt
      961 aattgcgttg cgctaatacg actcactata gggagaccac aacggtttcc ctccggctcc
     1021 ggaaataatt ttgtttaact ttaagaagga gatatacata tgggcagcag cgccagcgct
     1081 tggagccacc cgcagttcga gaaaggtgga ggttccggag gtggatcggg aggttcggcg
     1141 tggagccacc cgcagttcga aaaaagcagc ggatctctgg aagttctgtt ccaggggccc
     1201 ggatcccggt ccgacatatg agcgcgcgga attcaaaggc ctacgtcgac gagctcacta
     1261 gtcgcggccg ctttcgaatc tagagcctgc agtctcgaca agcttgtcga gaagtactag
     1321 aggatgtaca acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt
     1381 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     1441 tgaggggttt tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg
     1501 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg
     1561 aaccgacgac aagctgacga ccgggtctcc gcaagtggca cttttcgggg aaatgtgcgc
     1621 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa
     1681 ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     1741 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     1801 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     1861 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     1921 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     1981 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     2041 ttgcgcctga gcgagacgaa atacgcggtc gctgttaaaa ggacaattac aaacaggaat
     2101 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     2161 atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc
     2221 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     2281 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     2341 aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc
     2401 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     2461 cggcctagag caagacgttt cccgttgaat atggctcata ctcttccttt ttcaatatta
     2521 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttag
//
