LOCUS       Exported                5666 bp ds-DNA     circular SYN 19-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 3, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    71..104
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     117..138
                     /label=oWL130
                     /note="pLIB F"
     promoter        152..243
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_signal     194..196
                     /label=TSS
     misc_signal     244..246
                     /label=Polh-mut-ATG
     CDS             247..279
                     /label=Polh-leader
     primer_bind     complement(269..288)
                     /label=oWL728
                     /note="Description Gene Use Legacy Name"
     primer_bind     complement(274..333)
                     /label=oWL483
                     /note="Description:GST_rev Gene:GST Use:Mutagenesis Legacy 
                     Name"
     primer_bind     274..333
                     /label=oWL482
                     /note="Description:GST_fwdf Gene:GST Use:Mutagenesis Legacy
                     Name"
     CDS             286..939
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(286..322)
                     /label=oWL668
                     /note="Description:GST_D7Y_rev Gene:GST Use:Mutagenesis 
                     Legacy Name"
     primer_bind     complement(286..309)
                     /label=oWL220
                     /note="Description:GST - bp 1-24 - reverse Gene 
                     Use:Sequencing Legacy Name"
     primer_bind     289..318
                     /label=oWL770
                     /note="Description:C-GST_fwd Gene:GST Use:Cloning Legacy 
                     Name"
     primer_bind     289..318
                     /label=oWL769
                     /note="Description:3C_C-GST_fwd Gene:GST Use:Cloning Legacy
                     Name"
     primer_bind     295..331
                     /label=oWL669
                     /note="Description:GST_D7Y_fwd Gene:GST Use:Mutagenesis 
                     Legacy Name"
     primer_bind     complement(321..343)
                     /label=oWL534
                     /note="Description:GST_rev Gene:GST Use:Sequencing Legacy 
                     Name"
     misc_feature    370..372
                     /label=D29E point mutation (gaa)
     primer_bind     897..919
                     /label=oWL132
                     /note="GST_F"
     primer_bind     complement(922..940)
                     /label=oWL771
                     /note="Description:C-GST_rev Gene:GST Use:Cloning Legacy 
                     Name"
     primer_bind     complement(923..943)
                     /label=oWL855
                     /note="Description Gene Use:Sequencing Legacy Name"
     primer_bind     945..968
                     /label=oWL740
                     /note="Description Gene Use Legacy Name"
     primer_bind     945..963
                     /label=oWL736
                     /note="Description Gene Use Legacy Name"
     primer_bind     946..970
                     /label=oWL623
                     /note="Description:SNAP_no_Bam_f Gene Use Legacy Name"
     CDS             946..969
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     946..968
                     /label=oWL744
                     /note="Description Gene Use Legacy Name"
     primer_bind     complement(948..969)
                     /label=oWL678
                     /note="Description:Rv_C-MBP Gene:MBP Use:Cloning Legacy 
                     Name"
     primer_bind     complement(948..969)
                     /label=oWL013
                     /note="Spo11_GST_rev"
     primer_bind     complement(948..969)
                     /label=oWL016
                     /note="Spo11_38_GST_rev"
     primer_bind     953..993
                     /label=oWL804
                     /note="Description:Correct_3C_fwd Gene Use Legacy Name"
     misc_feature    955..975
                     /label=fwd primer overhang
     primer_bind     complement(992..1016)
                     /label=oWL622
                     /note="Description:pLIB_Strep_mutate_rev Gene Use Legacy 
                     Name"
     primer_bind     complement(1044..1068)
                     /label=oWL640
                     /note="AB5"
     primer_bind     1065..1110
                     /label=oWL621
                     /note="Description:pLIB_Strep_mutate_fwd Gene Use Legacy 
                     Name"
     primer_bind     1075..1105
                     /label=oWL726
                     /note="Description Gene Use Legacy Name"
     primer_bind     1075..1094
                     /label=oWL772
                     /note="Description:CTag_backbone_fwd Gene Use:Cloning 
                     Legacy Name"
     primer_bind     complement(1152..1171)
                     /label=oWL131
                     /note="pLIB R"
     polyA_signal    1198..1332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1404..1420)
                     /label=oWL227
                     /note="Description:STO720 (T7r) Gene:T7 terminator (pST44) 
                     Use:Sequencing Legacy Name:STO720"
     mobile_element  1442..1607
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1791..2245
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2271..2375
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2376..3236
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2874..2902
                     /label=oWL481
                     /note="Description:Amp_fwd Gene Use:Mutagenesis Legacy 
                     Name"
     primer_bind     complement(2874..2902)
                     /label=oWL480
                     /note="Description:Amp_rev Gene Use:Mutagenesis Legacy 
                     Name"
     rep_origin      3407..3995
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  4300..4524
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4591..5124)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     4820..4839
                     /label=oWL633
                     /note="Description:Gent.forw. Gene:Gentamycin resistance 
                     Use:Sequencing Legacy Name"
     primer_bind     complement(4980..5000)
                     /label=oWL634
                     /note="Description:Genta rev. Gene:gentamycine resistance 
                     Use:Sequencing Legacy Name"
     promoter        complement(5313..5341)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ctacagcttt gtttgtacta tcaacaggtt gaactgctga tcaacagatc ctctacgcgg
       61 ccgcggtacc ataacttcgt atagcataca ttatacgaag ttatctggtt ttactagtat
      121 cgattcgcga cctactccgg aatattaata gatcatggag ataattaaaa tgataaccat
      181 ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa aaaacctata
      241 aatattccgg attattcata ccgtcccacc atcgggcgcg gatctatgtc ccctatacta
      301 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
      361 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
      421 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
      481 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
      541 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
      601 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
      661 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
      721 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
      781 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
      841 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
      901 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatctgga agttctgttc
      961 caggggcccg gatcccggtc cgacatatga gcgcgcggaa ttcaaaggcc tacgtcgacg
     1021 agctcactag tcgcggccgc tttcgaatct agagcctgca gtctcgacaa gcttgtcgag
     1081 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     1141 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     1201 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1261 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1321 catgtctgga tctgatcact gcttgagcct agaagatccg gctgctaaca aagcccgaaa
     1381 ggaagctgag ttggctgctg ccaccgctga gcaataacta tcataacccc taggagatcc
     1441 gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc
     1501 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa
     1561 actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt
     1621 cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc
     1681 ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt tattaatgtt
     1741 tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg acgcgccctg
     1801 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1861 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1921 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1981 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     2041 atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg actcttgttc
     2101 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2161 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     2221 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2281 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2341 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2401 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2461 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2521 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2581 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2641 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2701 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2761 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2821 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2881 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2941 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3001 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3061 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3121 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3181 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3241 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3301 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3361 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3421 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3481 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3541 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3601 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3661 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3721 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3781 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3841 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3901 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3961 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4021 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4081 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4141 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     4201 ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg gcaaaatcgg
     4261 ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct
     4321 taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt
     4381 gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta
     4441 aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc
     4501 aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc
     4561 cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa
     4621 gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc
     4681 gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag
     4741 attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata
     4801 gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc
     4861 aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt
     4921 cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg
     4981 accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt
     5041 gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt
     5101 aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     5161 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac
     5221 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     5281 agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca
     5341 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
     5401 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
     5461 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc
     5521 ccttgcttca ggagatcggt agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc
     5581 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagg
     5641 actctagcta tagttctagt ggttgg
//
