LOCUS       Exported                5684 bp ds-DNA     circular SYN 04-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   John Weir
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 4, 2020 from SnapGene 4.3.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      72..660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  965..1189
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1256..1789)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(1978..2006)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    2402..2435
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        2483..2574
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2737..2760
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2767..3420
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKF
                     ELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYG
                     VSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLY
                     MDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     polyA_signal    3547..3681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  3791..3956
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4140..4594
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4620..4724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4725..5585
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
       61 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      181 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      721 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      781 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      841 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac
      901 cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag
      961 cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata
     1021 aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa
     1081 gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc
     1141 cgtcgtatta aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg
     1201 acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg
     1261 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
     1321 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
     1381 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
     1441 gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg
     1501 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
     1561 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
     1621 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
     1681 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
     1741 ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc
     1801 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     1861 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     1921 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     1981 ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg
     2041 cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac
     2101 gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac
     2161 ggcaaggtgc tgtgcacgga tctgcccttg cttcaggaga tcggtagacc tcggccgtcg
     2221 cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa
     2281 ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg gctacagctt
     2341 tgtttgtact atcaacaggt tgaactgctg atcaacagat cctctacgcg gccgcggtac
     2401 cataacttcg tatagcatac attatacgaa gttatctggt tttactagta tcgattcgcg
     2461 acctactccg gaatattaat agatcatgga gataattaaa atgataacca tctcgcaaat
     2521 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg
     2581 gattattcat accgtcccac catcgggcgc ggatcccggt ccgacatatg agcgcgcgga
     2641 attcaaaggc ctacgtcgac gagctcacta gtcgcggccg ctttcgaatc tagagcctgc
     2701 agtctcgaca agcttggaag cggatctgga tctggactgg aagttctgtt ccaggggccc
     2761 ggtagctccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt
     2821 cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat
     2881 aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt
     2941 gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac
     3001 aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt
     3061 ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa
     3121 gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat
     3181 aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt
     3241 gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt
     3301 aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata
     3361 gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaataa
     3421 taagtcgaga agtactagag gatcataatc agccatacca catttgtaga ggttttactt
     3481 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     3541 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3601 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3661 gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg ctgctaacaa
     3721 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactat cataacccct
     3781 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
     3841 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
     3901 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
     3961 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
     4021 gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt
     4081 attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatggga
     4141 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4201 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4261 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4321 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4381 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct taatagtgga
     4441 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4501 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4561 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4681 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     4741 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4801 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4861 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4921 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4981 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5041 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5101 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5161 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5221 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5281 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5341 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5401 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5461 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5521 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5581 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     5641 aatttaaaag gatctaggtg aagatccttt ttgataatct catg
//
