LOCUS       Exported                4522 bp ds-DNA     circular SYN 08-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 8, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..173
                     /label=ConLS'
     CDS             178..1203
                     /label=GFP drop-out
     misc_feature    1208..1397
                     /label=ConRE'
     misc_feature    1402..2511
                     /label=ScHIS3 cassette
     rep_origin      2516..2747
                     /label=ARS2
                     /note="ARS isolated from chromosome II"
     misc_feature    complement(2752..4522)
                     /label=kanR-ColE1
ORIGIN
        1 ccctgaattc gcatctagac tgatgagacg tggtagagcc acaaacagcc ggtacaagca
       61 acgatctcca ggaccatctg aatcatgcgc ggatgacacg aactcacgac ggcgatcaca
      121 gacattaacc cacagtacag acactgcgac aacgtggcaa ttcgtcgcaa tacaacgtga
      181 gaccgaaagt gaaacgtgat ttcatgcgtc attttgaaca ttttgtaaat cttatttaat
      241 aatgtgtgcg gcaattcaca tttaatttat gaatgttttc ttaacatcgc ggcaactcaa
      301 gaaacggcag gttcggatct tagctactag agaaagagga gaaatactag atgcgtaaag
      361 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg
      421 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc
      481 tgaagttcat ctgtactact ggtaaactgc cggttccttg gccgactctg gtaacgacgc
      541 tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct
      601 tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg
      661 gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg
      721 agctgaaagg cattgacttt aaagaggacg gcaatatcct gggccataag ctggaataca
      781 attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga
      841 attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc
      901 aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc
      961 aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg
     1021 taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgacca ggcatcaaat
     1081 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1141 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggtc
     1201 tcagctggaa atctgctcgt cagtggtgct cacactgacg aatcatgtac agatcatacc
     1261 gatgactgcc tggcgactca caactaagca agacagccgg aaccagcgcc ggcgaacacc
     1321 actgcatata tggcatatca caacagtcca cgtctcaagc agttacagag atgttacgaa
     1381 ccactagtgc actgcagtac aaacacagtc ctttcccgca attttctttt tctattactc
     1441 ttggcctcct ctagtacact ctatattttt ttatgcctcg gtaatgattt tcattttttt
     1501 ttttccacct agcggatgac tctttttttt tcttagcgat tggcattatc acataatgaa
     1561 ttatacatta tataaagtaa tgtgatttct tcgaagaata tactaaaaaa tgagcaggca
     1621 agataaacga aggcaaagat gacagagcag aaagccctag taaagcgtat tacaaatgaa
     1681 accaagattc agattgcgat ctctttaaag ggtggtcccc tagcgataga gcactcgatc
     1741 ttcccagaaa aagaggcaga agcagtagca gaacaggcca cacaatcgca agtgattaac
     1801 gtccacacag gtatagggtt tctggaccat atgatacatg ctctggccaa gcattccggc
     1861 tggtcgctaa tcgttgagtg cattggtgac ttacacatag acgaccatca caccactgag
     1921 gactgcggga ttgctctcgg tcaagctttt aaagaggccc taggggccgt gcgtggagta
     1981 aaaaggtttg gatcaggatt tgcgcctttg gatgaggcac tttccagagc ggtggttgat
     2041 ctttcgaaca ggccgtacgc agttgtcgaa cttggtttgc aaagggagaa agtaggtgat
     2101 ctctcttgcg agatgatccc gcattttctt gaaagctttg cagaggctag cagaattacc
     2161 ctccacgttg attgtctgcg aggcaagaat gatcatcacc gtagtgagag tgcgttcaag
     2221 gctcttgcgg ttgccataag agaagccacc tcgcccaatg gtaccaacga tgttccctcc
     2281 accaaaggtg ttcttatgta gtgacaccga ttatttaaag ctgctgcata cgatatatat
     2341 acatgtgtat atatgtatac ctatgaatgt cagtaagtat gtatacgaac agtatgatac
     2401 tgaagatgac aaggtaatgc atcattctat acgtgtcatt ctgaacgagg cgcgctttcc
     2461 ttttttcttt ttgctttttc tttttttttc tcttgaactc gacggatcat agagtcaata
     2521 ctttctaaca ttgaccattt tgcatcattt tgttacggaa aagtgctttc caatcatata
     2581 taattaaaac tagcggatca cattaaaaag ttcatccaaa gtgaaaaaac attctaaatt
     2641 tagtagacag caattgtttt aaaagggtgt tcctattttc atataacgga gagagttaag
     2701 cttcctgtat acaattagca aatattttca ctatagagac ctgggacccg agcggccgcg
     2761 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
     2821 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     2881 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     2941 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     3001 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
     3061 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     3121 accaaaccgt tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta
     3181 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     3241 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     3301 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     3361 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     3421 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     3481 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     3541 gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc
     3601 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
     3661 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
     3721 aatcgaactt ttgctgagtt gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt
     3781 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3841 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3901 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3961 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4021 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4081 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4141 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4201 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4261 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4321 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4381 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4441 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4501 ctgtggataa ccgtgcggcc gc
//
