LOCUS       Exported                4803 bp ds-DNA     circular SYN 08-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 8, 2020 from SnapGene Viewer 5.1.5
            https://www.snapgene.com
COMMENT     [DEFINITION]: synthetic circular DNA
[LOCUS]: Exported
[ACCESSION]: 
            .
[SOURCE]: 1..5185
[CDS]: 178..1203
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..173
                     /label=ConLS'
     CDS             178..1203
                     /label=GFP drop-out
     misc_feature    1208..1397
                     /label=ConRE'
     misc_feature    1402..2792
                     /label=KanMX cassette
                     /note="label=hphMX cassette - 1402..3015"
     rep_origin      2797..3028
                     /label=ARS2
                     /note="Minimal ARS from chromosome II"
     misc_feature    complement(3033..4803)
                     /label=kanR-ColE1
ORIGIN
        1 ccctgaattc gcatctagac tgatgagacg tggtagagcc acaaacagcc ggtacaagca
       61 acgatctcca ggaccatctg aatcatgcgc ggatgacacg aactcacgac ggcgatcaca
      121 gacattaacc cacagtacag acactgcgac aacgtggcaa ttcgtcgcaa tacaacgtga
      181 gaccgaaagt gaaacgtgat ttcatgcgtc attttgaaca ttttgtaaat cttatttaat
      241 aatgtgtgcg gcaattcaca tttaatttat gaatgttttc ttaacatcgc ggcaactcaa
      301 gaaacggcag gttcggatct tagctactag agaaagagga gaaatactag atgcgtaaag
      361 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg
      421 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc
      481 tgaagttcat ctgtactact ggtaaactgc cggttccttg gccgactctg gtaacgacgc
      541 tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct
      601 tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg
      661 gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg
      721 agctgaaagg cattgacttt aaagaggacg gcaatatcct gggccataag ctggaataca
      781 attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga
      841 attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc
      901 aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc
      961 aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg
     1021 taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgacca ggcatcaaat
     1081 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1141 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggtc
     1201 tcagctggaa atctgctcgt cagtggtgct cacactgacg aatcatgtac agatcatacc
     1261 gatgactgcc tggcgactca caactaagca agacagccgg aaccagcgcc ggcgaacacc
     1321 actgcatata tggcatatca caacagtcca cgtctcaagc agttacagag atgttacgaa
     1381 ccactagtgc actgcagtac aagcttgcct cgtccccgcc gggtcacccg gccagcgaca
     1441 tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg
     1501 actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt
     1561 ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctccagacc tgcgagcagg
     1621 gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata
     1681 taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc
     1741 taggatacag ttctcacatc acatccgaac ataaacaaaa atgggtaagg aaaagactca
     1801 cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1861 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
     1921 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1981 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2041 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag cattccaggt
     2101 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2161 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2221 ggctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2281 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc
     2341 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2401 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2461 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2521 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2581 gtttttctaa agtaactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     2641 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     2701 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
     2761 tcaatcgtat gtgaatgctg gtcgctatac tggagtcaat actttctaac attgaccatt
     2821 ttgcatcatt ttgttacgga aaagtgcttt ccaatcatat ataattaaaa ctagcggatc
     2881 acattaaaaa gttcatccaa agtgaaaaaa cattctaaat ttagtagaca gcaattgttt
     2941 taaaagggtg ttcctatttt catataacgg agagagttaa gcttcctgta tacaattagc
     3001 aaatattttc actatagaga cctgggaccc gagcggccgc gtgttacaac caattaacca
     3061 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     3121 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     3181 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     3241 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     3301 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
     3361 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     3421 gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     3481 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     3541 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     3601 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     3661 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     3721 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     3781 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     3841 atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     3901 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     3961 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     4021 tgaaggatca gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4081 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4141 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4201 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4261 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4321 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4381 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4441 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4501 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4561 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4621 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4681 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4741 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtgcggc
     4801 cgc
//
